Characterization of bacterial DNA binding to human neutrophil surface

Bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Neutrophil activation does not require DNA internalization, suggesting that it results from the interaction of bacterial DNA with a neutrophil surface receptor. The aim of this study was to characterize the interactio...

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Autores principales:	Fuxman Bass, Juan Ignacio, Gabelloni, María Laura, Vermeulen, Mónica, Russo, Daniela Marta, Zorreguieta, Angeles, Trevani, Analía S.
Publicado:	2008
Materias:	Bacterial DNA binding Biofilm CpG Inflammation Neutrophils TLR9 bacterial DNA chemoattractant deoxyribonuclease article biotinylation cell culture cell isolation cell migration cell surface controlled study DNA binding human human cell neutrophil priority journal Base Sequence Biofilms Cells, Cultured DNA Primers DNA, Bacterial Escherichia coli Humans
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236837_v88_n9_p926_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00236837_v88_n9_p926_FuxmanBass
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00236837_v88_n9_p926_FuxmanBass
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spelling	paper:paper_00236837_v88_n9_p926_FuxmanBass2023-06-08T14:51:53Z Characterization of bacterial DNA binding to human neutrophil surface Fuxman Bass, Juan Ignacio Gabelloni, María Laura Vermeulen, Mónica Russo, Daniela Marta Zorreguieta, Angeles Trevani, Analía S. Bacterial DNA binding Biofilm CpG Inflammation Neutrophils TLR9 bacterial DNA chemoattractant deoxyribonuclease article biotinylation cell culture cell isolation cell migration cell surface controlled study DNA binding human human cell neutrophil priority journal Base Sequence Biofilms Cells, Cultured DNA Primers DNA, Bacterial Escherichia coli Humans Neutrophils Bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Neutrophil activation does not require DNA internalization, suggesting that it results from the interaction of bacterial DNA with a neutrophil surface receptor. The aim of this study was to characterize the interaction of bacterial DNA with the neutrophil surface. Bacterial DNA binding showed saturation and was inhibited by unlabeled DNA but not by other polyanions like yeast tRNA and poly-A. Resembling the conditions under which bacterial DNA triggers neutrophil activation, binding was not modified in the presence or absence of calcium, magnesium or serum. Treatment of neutrophils with proteases not only dramatically reduced bacterial DNA binding but also inhibited neutrophil activation induced by bacterial DNA. Experiments performed with DNA samples of different lengths obtained after digestion of bacterial DNA with DNase showed that only DNA fragments greater than ≈170-180 nucleotides competed bacterial DNA binding and retained the ability to trigger cell activation. Treatment of neutrophils with chemoattractants or conventional agonists significantly increased bacterial DNA binding. Moreover, neutrophils that underwent transmigration through human endothelial cell monolayers even in the absence of chemoattractants, exhibited higher binding levels of bacterial DNA. Together, our findings provide evidence that binding of bacterial DNA to neutrophils is a receptor-mediated process that conditions the ability of DNA to trigger cell activation. We speculate that neutrophil recognition of bacterial DNA might be modulated by the balance of agonists present at inflammatory foci. This effect might be relevant in bacterial infections with a biofilm etiology, in which extracellular DNA could function as a potent neutrophil agonist. © 2008 USCAP, Inc All rights reserved. Fil:Fuxman Bass, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabelloni, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vermeulen, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Russo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, Á. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Trevani, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236837_v88_n9_p926_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00236837_v88_n9_p926_FuxmanBass
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacterial DNA binding Biofilm CpG Inflammation Neutrophils TLR9 bacterial DNA chemoattractant deoxyribonuclease article biotinylation cell culture cell isolation cell migration cell surface controlled study DNA binding human human cell neutrophil priority journal Base Sequence Biofilms Cells, Cultured DNA Primers DNA, Bacterial Escherichia coli Humans Neutrophils
spellingShingle	Bacterial DNA binding Biofilm CpG Inflammation Neutrophils TLR9 bacterial DNA chemoattractant deoxyribonuclease article biotinylation cell culture cell isolation cell migration cell surface controlled study DNA binding human human cell neutrophil priority journal Base Sequence Biofilms Cells, Cultured DNA Primers DNA, Bacterial Escherichia coli Humans Neutrophils Fuxman Bass, Juan Ignacio Gabelloni, María Laura Vermeulen, Mónica Russo, Daniela Marta Zorreguieta, Angeles Trevani, Analía S. Characterization of bacterial DNA binding to human neutrophil surface
topic_facet	Bacterial DNA binding Biofilm CpG Inflammation Neutrophils TLR9 bacterial DNA chemoattractant deoxyribonuclease article biotinylation cell culture cell isolation cell migration cell surface controlled study DNA binding human human cell neutrophil priority journal Base Sequence Biofilms Cells, Cultured DNA Primers DNA, Bacterial Escherichia coli Humans Neutrophils
description	Bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Neutrophil activation does not require DNA internalization, suggesting that it results from the interaction of bacterial DNA with a neutrophil surface receptor. The aim of this study was to characterize the interaction of bacterial DNA with the neutrophil surface. Bacterial DNA binding showed saturation and was inhibited by unlabeled DNA but not by other polyanions like yeast tRNA and poly-A. Resembling the conditions under which bacterial DNA triggers neutrophil activation, binding was not modified in the presence or absence of calcium, magnesium or serum. Treatment of neutrophils with proteases not only dramatically reduced bacterial DNA binding but also inhibited neutrophil activation induced by bacterial DNA. Experiments performed with DNA samples of different lengths obtained after digestion of bacterial DNA with DNase showed that only DNA fragments greater than ≈170-180 nucleotides competed bacterial DNA binding and retained the ability to trigger cell activation. Treatment of neutrophils with chemoattractants or conventional agonists significantly increased bacterial DNA binding. Moreover, neutrophils that underwent transmigration through human endothelial cell monolayers even in the absence of chemoattractants, exhibited higher binding levels of bacterial DNA. Together, our findings provide evidence that binding of bacterial DNA to neutrophils is a receptor-mediated process that conditions the ability of DNA to trigger cell activation. We speculate that neutrophil recognition of bacterial DNA might be modulated by the balance of agonists present at inflammatory foci. This effect might be relevant in bacterial infections with a biofilm etiology, in which extracellular DNA could function as a potent neutrophil agonist. © 2008 USCAP, Inc All rights reserved.
author	Fuxman Bass, Juan Ignacio Gabelloni, María Laura Vermeulen, Mónica Russo, Daniela Marta Zorreguieta, Angeles Trevani, Analía S.
author_facet	Fuxman Bass, Juan Ignacio Gabelloni, María Laura Vermeulen, Mónica Russo, Daniela Marta Zorreguieta, Angeles Trevani, Analía S.
author_sort	Fuxman Bass, Juan Ignacio
title	Characterization of bacterial DNA binding to human neutrophil surface
title_short	Characterization of bacterial DNA binding to human neutrophil surface
title_full	Characterization of bacterial DNA binding to human neutrophil surface
title_fullStr	Characterization of bacterial DNA binding to human neutrophil surface
title_full_unstemmed	Characterization of bacterial DNA binding to human neutrophil surface
title_sort	characterization of bacterial dna binding to human neutrophil surface
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236837_v88_n9_p926_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00236837_v88_n9_p926_FuxmanBass
work_keys_str_mv	AT fuxmanbassjuanignacio characterizationofbacterialdnabindingtohumanneutrophilsurface AT gabellonimarialaura characterizationofbacterialdnabindingtohumanneutrophilsurface AT vermeulenmonica characterizationofbacterialdnabindingtohumanneutrophilsurface AT russodanielamarta characterizationofbacterialdnabindingtohumanneutrophilsurface AT zorreguietaangeles characterizationofbacterialdnabindingtohumanneutrophilsurface AT trevanianalias characterizationofbacterialdnabindingtohumanneutrophilsurface
_version_	1768546709114191872

Characterization of bacterial DNA binding to human neutrophil surface

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