Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry

Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cer...

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Autores principales:	Schenk, Marcela Liliana, Raffellini, Silvia Mónica, Guerrero, Sandra, Alzamora, Stella Maris
Publicado:	2011
Materias:	E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light S.cerevisiae Diseases Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Listeria innocua Saccharomyces cerevisiae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v44_n1_p191_Schenk http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00236438_v44_n1_p191_Schenk
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spelling	paper:paper_00236438_v44_n1_p191_Schenk2023-06-08T14:51:45Z Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry Schenk, Marcela Liliana Raffellini, Silvia Mónica Guerrero, Sandra Alzamora, Stella Maris E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light E. coli Inactivation L. innocua S.cerevisiae UV-C light Diseases Flow cytometry Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Escherichia coli Listeria innocua Saccharomyces cerevisiae Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cerevisiae KE162 cells by applying flow cytometry technique. The UV-C doses, obtained by altering the exposure time and measured by the iodide-iodate chemical actinometer, ranged between 0 and 5 kJ/m2. E. coli; L. innocua and S. cerevisiae populations were quantified by plate count technique. For flow cytometry studies, cells were labeled with fluorescein diacetate (FDA) for detecting membrane integrity and esterase activity, and with propidium iodide (PI) for monitoring membrane integrity. The results showed that mechanisms of cellular damage differed according to time of exposure to ultraviolet radiation and the organism tested. E. coli and S. cerevisiae sub-populations with PI increased within the first minutes of UV-C treatment, without much change afterwards. On the contrary, FCM was used to detect the inactivation of those L. innocua sub-populations of viable microorganisms (maintaining metabolic activity) which were non-culturable due to membrane rupture and thus not detectable by viable plate count technique. © 2010 Elsevier Ltd. Fil:Schenk, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Raffellini, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerrero, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alzamora, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v44_n1_p191_Schenk http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light E. coli Inactivation L. innocua S.cerevisiae UV-C light Diseases Flow cytometry Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Escherichia coli Listeria innocua Saccharomyces cerevisiae
spellingShingle	E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light E. coli Inactivation L. innocua S.cerevisiae UV-C light Diseases Flow cytometry Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Escherichia coli Listeria innocua Saccharomyces cerevisiae Schenk, Marcela Liliana Raffellini, Silvia Mónica Guerrero, Sandra Alzamora, Stella Maris Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
topic_facet	E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light E. coli Inactivation L. innocua S.cerevisiae UV-C light Diseases Flow cytometry Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Escherichia coli Listeria innocua Saccharomyces cerevisiae
description	Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cerevisiae KE162 cells by applying flow cytometry technique. The UV-C doses, obtained by altering the exposure time and measured by the iodide-iodate chemical actinometer, ranged between 0 and 5 kJ/m2. E. coli; L. innocua and S. cerevisiae populations were quantified by plate count technique. For flow cytometry studies, cells were labeled with fluorescein diacetate (FDA) for detecting membrane integrity and esterase activity, and with propidium iodide (PI) for monitoring membrane integrity. The results showed that mechanisms of cellular damage differed according to time of exposure to ultraviolet radiation and the organism tested. E. coli and S. cerevisiae sub-populations with PI increased within the first minutes of UV-C treatment, without much change afterwards. On the contrary, FCM was used to detect the inactivation of those L. innocua sub-populations of viable microorganisms (maintaining metabolic activity) which were non-culturable due to membrane rupture and thus not detectable by viable plate count technique. © 2010 Elsevier Ltd.
author	Schenk, Marcela Liliana Raffellini, Silvia Mónica Guerrero, Sandra Alzamora, Stella Maris
author_facet	Schenk, Marcela Liliana Raffellini, Silvia Mónica Guerrero, Sandra Alzamora, Stella Maris
author_sort	Schenk, Marcela Liliana
title	Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_short	Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_full	Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_fullStr	Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_full_unstemmed	Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_sort	inactivation of escherichia coli, listeria innocua and saccharomyces cerevisiae by uv-c light: study of cell injury by flow cytometry
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v44_n1_p191_Schenk http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
work_keys_str_mv	AT schenkmarcelaliliana inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry AT raffellinisilviamonica inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry AT guerrerosandra inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry AT alzamorastellamaris inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry
_version_	1768546573045727232

Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry

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