Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure

Textural, histological and biochemical characteristics of cucumber (Cucumis sativus L) under different tissue turgor pressures were studied after equilibration of mesocarp tissue cylinders in various polyethylene glycol 400 (PEG) solutions. Tissue swelling was observed for all hypotonic solutions. C...

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Autores principales:	Gamba, Gisela, Gerschenson, Lía Noemí, Rojas, Ana María Luisa
Publicado:	2003
Materias:	Cucumber Enzymes Texture Turgor pressure fruit Cucumis Cucumis sativus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v83_n7_p731_Sajnin http://hdl.handle.net/20.500.12110/paper_00225142_v83_n7_p731_Sajnin
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00225142_v83_n7_p731_Sajnin
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spelling	paper:paper_00225142_v83_n7_p731_Sajnin2023-06-08T14:51:18Z Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure Gamba, Gisela Gerschenson, Lía Noemí Rojas, Ana María Luisa Cucumber Enzymes Texture Turgor pressure fruit Cucumis Cucumis sativus Textural, histological and biochemical characteristics of cucumber (Cucumis sativus L) under different tissue turgor pressures were studied after equilibration of mesocarp tissue cylinders in various polyethylene glycol 400 (PEG) solutions. Tissue swelling was observed for all hypotonic solutions. Cell membrane vitality was detected in all cucumber tissues where significant values of initial firmness (fi) and residual relaxation force (FR) were found. Good turgor status was also associated with higher values of these parameters, observing higher values of the textural parameters for higher turgor. Cell wall integrity was observed through Calcofluor fluorescence assay in tissue equilibrated in hypotonic, isotonic and hypertonic solutions, although a lower fluorescence intensity was observed in tissue equilibrated with 0molm-3 PEG solution, probably owing to some cell bursting. Calcium bridges between pectic polymers were proven to be responsible for cell-cell adhesion and tissue integrity of cucumber mesocarp tissue. Compression curves obtained for raw tissue showed a stage of fracture, and its loss after immersion might be attributed to cell-cell adhesion weakness due to immersion in calcium-free PEG solutions. Peroxidase and polygalacturonase increased after cucumber equilibration in the solutions, probably expressing tissue injury. Cucumber textural behaviour was very much affected by immersion in calcium-free solutions, determining the loss of fracture stage and a great influence of turgor pressure on the textural response of soaked tissue. © 2003 Society of Chemical Industry. Fil:Gamba, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerschenson, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v83_n7_p731_Sajnin http://hdl.handle.net/20.500.12110/paper_00225142_v83_n7_p731_Sajnin
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cucumber Enzymes Texture Turgor pressure fruit Cucumis Cucumis sativus
spellingShingle	Cucumber Enzymes Texture Turgor pressure fruit Cucumis Cucumis sativus Gamba, Gisela Gerschenson, Lía Noemí Rojas, Ana María Luisa Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
topic_facet	Cucumber Enzymes Texture Turgor pressure fruit Cucumis Cucumis sativus
description	Textural, histological and biochemical characteristics of cucumber (Cucumis sativus L) under different tissue turgor pressures were studied after equilibration of mesocarp tissue cylinders in various polyethylene glycol 400 (PEG) solutions. Tissue swelling was observed for all hypotonic solutions. Cell membrane vitality was detected in all cucumber tissues where significant values of initial firmness (fi) and residual relaxation force (FR) were found. Good turgor status was also associated with higher values of these parameters, observing higher values of the textural parameters for higher turgor. Cell wall integrity was observed through Calcofluor fluorescence assay in tissue equilibrated in hypotonic, isotonic and hypertonic solutions, although a lower fluorescence intensity was observed in tissue equilibrated with 0molm-3 PEG solution, probably owing to some cell bursting. Calcium bridges between pectic polymers were proven to be responsible for cell-cell adhesion and tissue integrity of cucumber mesocarp tissue. Compression curves obtained for raw tissue showed a stage of fracture, and its loss after immersion might be attributed to cell-cell adhesion weakness due to immersion in calcium-free PEG solutions. Peroxidase and polygalacturonase increased after cucumber equilibration in the solutions, probably expressing tissue injury. Cucumber textural behaviour was very much affected by immersion in calcium-free solutions, determining the loss of fracture stage and a great influence of turgor pressure on the textural response of soaked tissue. © 2003 Society of Chemical Industry.
author	Gamba, Gisela Gerschenson, Lía Noemí Rojas, Ana María Luisa
author_facet	Gamba, Gisela Gerschenson, Lía Noemí Rojas, Ana María Luisa
author_sort	Gamba, Gisela
title	Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
title_short	Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
title_full	Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
title_fullStr	Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
title_full_unstemmed	Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure
title_sort	textural, histological and biochemical changes in cucumber (cucumis sativus l) due to immersion and variations in turgor pressure
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v83_n7_p731_Sajnin http://hdl.handle.net/20.500.12110/paper_00225142_v83_n7_p731_Sajnin
work_keys_str_mv	AT gambagisela texturalhistologicalandbiochemicalchangesincucumbercucumissativuslduetoimmersionandvariationsinturgorpressure AT gerschensonlianoemi texturalhistologicalandbiochemicalchangesincucumbercucumissativuslduetoimmersionandvariationsinturgorpressure AT rojasanamarialuisa texturalhistologicalandbiochemicalchangesincucumbercucumissativuslduetoimmersionandvariationsinturgorpressure
_version_	1768542023002882048

Textural, histological and biochemical changes in cucumber (Cucumis sativus L) due to immersion and variations in turgor pressure

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