Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone

Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4...

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Autores principales: Cozza, Eduardo Néstor, Ceballos, Nora Raquel, Vila, María del Carmen
Publicado: 1987
Materias:
18 hydroxycorticosterone
corticosterone
drug derivative
adrenal gland
animal
article
cell fractionation
cell nucleus
cytosol
kinetics
metabolism
microsome
mitochondrion
rat
rat strain
solubility
18-Hydroxycorticosterone
Adrenal Glands
Animal
Cell Nucleus
Corticosterone
Cytosol
Kinetics
Microsomes
Mitochondria
Rats
Rats, Inbred Strains
Solubility
Subcellular Fractions
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v28_n5_p543_Cozza
http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00224731_v28_n5_p543_Cozza
record_format dspace
spelling paper:paper_00224731_v28_n5_p543_Cozza2023-06-08T14:51:03Z Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone Cozza, Eduardo Néstor Ceballos, Nora Raquel Vila, María del Carmen 18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4 than at pH 4.8. Not only 18-OH-B but also its less polar anhydride 18-DAL (18-Deoxyaldosterone) were good precursors. SM was reverted to these precursors both enzymatically and spontaneously, 4.8 being a more suitable pH for this reversion than 7.4. Trapping experiments demonstrated a sequence comprising, in this order, the following echelons: SM, 18-OH-B, 18-DAL, Aldosterone. The first two steps are reversible and the last two ones depend on proton concentrations. It is postulated that SM could be on a dead-end to which 18-OH-B could be deviated if Aldosterone biosynthesis became temporarily unnecessary. Also, that 18-OH-B may convert to either 18-DAL or SM for selective membrane transports, according to homeostatic requirements. © 1987. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Carmen Vila, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v28_n5_p543_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 18 hydroxycorticosterone
corticosterone
drug derivative
adrenal gland
animal
article
cell fractionation
cell nucleus
cytosol
kinetics
metabolism
microsome
mitochondrion
rat
rat strain
solubility
18-Hydroxycorticosterone
Adrenal Glands
Animal
Cell Nucleus
Corticosterone
Cytosol
Kinetics
Microsomes
Mitochondria
Rats
Rats, Inbred Strains
Solubility
Subcellular Fractions
Support, Non-U.S. Gov't
spellingShingle 18 hydroxycorticosterone
corticosterone
drug derivative
adrenal gland
animal
article
cell fractionation
cell nucleus
cytosol
kinetics
metabolism
microsome
mitochondrion
rat
rat strain
solubility
18-Hydroxycorticosterone
Adrenal Glands
Animal
Cell Nucleus
Corticosterone
Cytosol
Kinetics
Microsomes
Mitochondria
Rats
Rats, Inbred Strains
Solubility
Subcellular Fractions
Support, Non-U.S. Gov't
Cozza, Eduardo Néstor
Ceballos, Nora Raquel
Vila, María del Carmen
Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
topic_facet 18 hydroxycorticosterone
corticosterone
drug derivative
adrenal gland
animal
article
cell fractionation
cell nucleus
cytosol
kinetics
metabolism
microsome
mitochondrion
rat
rat strain
solubility
18-Hydroxycorticosterone
Adrenal Glands
Animal
Cell Nucleus
Corticosterone
Cytosol
Kinetics
Microsomes
Mitochondria
Rats
Rats, Inbred Strains
Solubility
Subcellular Fractions
Support, Non-U.S. Gov't
description Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4 than at pH 4.8. Not only 18-OH-B but also its less polar anhydride 18-DAL (18-Deoxyaldosterone) were good precursors. SM was reverted to these precursors both enzymatically and spontaneously, 4.8 being a more suitable pH for this reversion than 7.4. Trapping experiments demonstrated a sequence comprising, in this order, the following echelons: SM, 18-OH-B, 18-DAL, Aldosterone. The first two steps are reversible and the last two ones depend on proton concentrations. It is postulated that SM could be on a dead-end to which 18-OH-B could be deviated if Aldosterone biosynthesis became temporarily unnecessary. Also, that 18-OH-B may convert to either 18-DAL or SM for selective membrane transports, according to homeostatic requirements. © 1987.
author Cozza, Eduardo Néstor
Ceballos, Nora Raquel
Vila, María del Carmen
author_facet Cozza, Eduardo Néstor
Ceballos, Nora Raquel
Vila, María del Carmen
author_sort Cozza, Eduardo Néstor
title Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_short Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_full Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_fullStr Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_full_unstemmed Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_sort convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v28_n5_p543_Cozza
http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
work_keys_str_mv AT cozzaeduardonestor convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone
AT ceballosnoraraquel convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone
AT vilamariadelcarmen convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone
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