Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals

Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h...

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Detalles Bibliográficos
Autores principales:	Cozza, Eduardo Néstor, Burton, Gerardo, Ceballos, Nora Raquel
Publicado:	1985
Materias:	18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza
Aporte de:	Aportado por : Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires .

id	paper:paper_00224731_v22_n5_p665_Cozza
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia
spellingShingle	18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
topic_facet	18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia
description	Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985.
author	Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel
author_facet	Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel
author_sort	Cozza, Eduardo Néstor
title	Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_short	Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_full	Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_fullStr	Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_full_unstemmed	Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_sort	eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza
work_keys_str_mv	AT cozzaeduardonestor eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals AT burtongerardo eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals AT ceballosnoraraquel eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
bdutipo_str	Repositorios
_version_	1764820558450524161

Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals

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