Estrogen receptor in rat pancreatic islets

The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytos...

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Autores principales:	Tesone, Marta, Chazenbalk, Gregorio Daniel, Charreau, Eduardo Hernán
Publicado:	1979
Materias:	estradiol estrogen receptor animal article binding competition castration cytosol experimental diabetes mellitus female isolation and purification kinetics metabolism pancreas islet pregnancy rat Animal Binding, Competitive Castration Cytosol Diabetes Mellitus, Experimental Estradiol Female Islets of Langerhans Kinetics Pregnancy Rats Receptors, Estrogen
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n3_p1309_Tesone http://hdl.handle.net/20.500.12110/paper_00224731_v11_n3_p1309_Tesone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00224731_v11_n3_p1309_Tesone
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spelling	paper:paper_00224731_v11_n3_p1309_Tesone2023-06-08T14:50:58Z Estrogen receptor in rat pancreatic islets Tesone, Marta Chazenbalk, Gregorio Daniel Charreau, Eduardo Hernán estradiol estrogen receptor animal article binding competition castration cytosol experimental diabetes mellitus female isolation and purification kinetics metabolism pancreas islet pregnancy rat Animal Binding, Competitive Castration Cytosol Diabetes Mellitus, Experimental Estradiol Female Islets of Langerhans Kinetics Pregnancy Rats Receptors, Estrogen The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytosol binding reaction, measured by charcoal-dextran assay, indicated a single class of estradiol-binding sites having high affinity (Kd = 2.9 × 10-8 M at 0°C). The number of binding sites was calculated to be 29.6 fmol/mg cytosol protein in whole pancreas and 170 fmol/mg cytosol protein in isolated pancreatic islets after collagenase treatment. Competition studies indicated high specificity for the binding reaction, since excess (100-fold) unlabelled estrogens, diethylstilbestrol and the antiestrogen nafoxidine, all significantly reduced the binding of [3H]-estradiol. On the other hand, the nonestrogenic steroids dihydrotestosterone, corticosterone and progesterone had no significant effects on [3H]-estradiol binding. The complex had a sedimentation coefficient of 4-5 S in sucrose density gradient centrifugation in low salt. In streptozotocin-diabetic and in 3-wk pregnant rats a significant decrease in the binding of estradiol to pancreas islet cytosol was found. © 1979. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chazenbalk, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1979 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n3_p1309_Tesone http://hdl.handle.net/20.500.12110/paper_00224731_v11_n3_p1309_Tesone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	estradiol estrogen receptor animal article binding competition castration cytosol experimental diabetes mellitus female isolation and purification kinetics metabolism pancreas islet pregnancy rat Animal Binding, Competitive Castration Cytosol Diabetes Mellitus, Experimental Estradiol Female Islets of Langerhans Kinetics Pregnancy Rats Receptors, Estrogen
spellingShingle	estradiol estrogen receptor animal article binding competition castration cytosol experimental diabetes mellitus female isolation and purification kinetics metabolism pancreas islet pregnancy rat Animal Binding, Competitive Castration Cytosol Diabetes Mellitus, Experimental Estradiol Female Islets of Langerhans Kinetics Pregnancy Rats Receptors, Estrogen Tesone, Marta Chazenbalk, Gregorio Daniel Charreau, Eduardo Hernán Estrogen receptor in rat pancreatic islets
topic_facet	estradiol estrogen receptor animal article binding competition castration cytosol experimental diabetes mellitus female isolation and purification kinetics metabolism pancreas islet pregnancy rat Animal Binding, Competitive Castration Cytosol Diabetes Mellitus, Experimental Estradiol Female Islets of Langerhans Kinetics Pregnancy Rats Receptors, Estrogen
description	The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytosol binding reaction, measured by charcoal-dextran assay, indicated a single class of estradiol-binding sites having high affinity (Kd = 2.9 × 10-8 M at 0°C). The number of binding sites was calculated to be 29.6 fmol/mg cytosol protein in whole pancreas and 170 fmol/mg cytosol protein in isolated pancreatic islets after collagenase treatment. Competition studies indicated high specificity for the binding reaction, since excess (100-fold) unlabelled estrogens, diethylstilbestrol and the antiestrogen nafoxidine, all significantly reduced the binding of [3H]-estradiol. On the other hand, the nonestrogenic steroids dihydrotestosterone, corticosterone and progesterone had no significant effects on [3H]-estradiol binding. The complex had a sedimentation coefficient of 4-5 S in sucrose density gradient centrifugation in low salt. In streptozotocin-diabetic and in 3-wk pregnant rats a significant decrease in the binding of estradiol to pancreas islet cytosol was found. © 1979.
author	Tesone, Marta Chazenbalk, Gregorio Daniel Charreau, Eduardo Hernán
author_facet	Tesone, Marta Chazenbalk, Gregorio Daniel Charreau, Eduardo Hernán
author_sort	Tesone, Marta
title	Estrogen receptor in rat pancreatic islets
title_short	Estrogen receptor in rat pancreatic islets
title_full	Estrogen receptor in rat pancreatic islets
title_fullStr	Estrogen receptor in rat pancreatic islets
title_full_unstemmed	Estrogen receptor in rat pancreatic islets
title_sort	estrogen receptor in rat pancreatic islets
publishDate	1979
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n3_p1309_Tesone http://hdl.handle.net/20.500.12110/paper_00224731_v11_n3_p1309_Tesone
work_keys_str_mv	AT tesonemarta estrogenreceptorinratpancreaticislets AT chazenbalkgregoriodaniel estrogenreceptorinratpancreaticislets AT charreaueduardohernan estrogenreceptorinratpancreaticislets
_version_	1768545360428400640

Estrogen receptor in rat pancreatic islets

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