Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice
The role of P/Q- and T-type calcium channels in the rhythmic oscillatory behaviour of inferior olive (IO) neurons was investigated in mutant mice. Mice lacking either the CaV2.1 gene of the pore-forming α1A subunit for P/Q-type calcium channel, or the CaV3.1 gene of the pore-forming α1G subunit for...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v588_n16_p3031_Choi http://hdl.handle.net/20.500.12110/paper_00223751_v588_n16_p3031_Choi |
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paper:paper_00223751_v588_n16_p3031_Choi2023-06-08T14:50:27Z Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice calcium channel calcium channel Cav2.1 calcium channel Cav3.1 calcium channel P type calcium channel Q type calcium channel T type unclassified drug calcium channel P type calcium channel Q type calcium channel T type animal cell animal experiment animal tissue article controlled study electric potential inferior olive mathematical model membrane potential mouse nerve cell nonhuman oscillation priority journal action potential amplitude Article cell function cellular parameters female inferior olive intracellular recording male membrane steady potential molecular imaging nerve cell membrane potential protein function protein transport signal transduction sinusoidal subthreshold membrane potential oscillation spike wave Animals Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, T-Type Calcium Signaling Computer Simulation Kinetics Membrane Potentials Mice Mice, Knockout Models, Neurological Neurons Olivary Nucleus The role of P/Q- and T-type calcium channels in the rhythmic oscillatory behaviour of inferior olive (IO) neurons was investigated in mutant mice. Mice lacking either the CaV2.1 gene of the pore-forming α1A subunit for P/Q-type calcium channel, or the CaV3.1 gene of the pore-forming α1G subunit for T-type calcium channel were used. In vitro intracellular recording from IO neurons reveals that the amplitude and frequency of sinusoidal subthreshold oscillations (SSTOs) were reduced in the CaV2.1-/- mice. In the CaV3.1-/- mice, IO neurons also showed altered patterns of SSTOs and the probability of SSTO generation was significantly lower (15%, 5 of 34 neurons) than that of wild-type (78%, 31 of 40 neurons) or CaV2.1-/- mice (73%, 22 of 30 neurons). In addition, the low-threshold calcium spike and the sustained endogenous oscillation following rebound potentials were absent in IO neurons from CaV3.1-/- mice. Moreover, the phase-reset dynamics of oscillatory properties of single neurons and neuronal clusters in IO were remarkably altered in both CaV2.1-/- and CaV3.1-/- mice. These results suggest that both α1A P/Q- and α1G T-type calcium channels are required for the dynamic control of neuronal oscillations in the IO. These findings were supported by results from a mathematical IO neuronal model that incorporated T and P/Q channel kinetics. © 2010 The Authors. Journal compilation © 2010 The Physiological Society. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v588_n16_p3031_Choi http://hdl.handle.net/20.500.12110/paper_00223751_v588_n16_p3031_Choi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
calcium channel calcium channel Cav2.1 calcium channel Cav3.1 calcium channel P type calcium channel Q type calcium channel T type unclassified drug calcium channel P type calcium channel Q type calcium channel T type animal cell animal experiment animal tissue article controlled study electric potential inferior olive mathematical model membrane potential mouse nerve cell nonhuman oscillation priority journal action potential amplitude Article cell function cellular parameters female inferior olive intracellular recording male membrane steady potential molecular imaging nerve cell membrane potential protein function protein transport signal transduction sinusoidal subthreshold membrane potential oscillation spike wave Animals Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, T-Type Calcium Signaling Computer Simulation Kinetics Membrane Potentials Mice Mice, Knockout Models, Neurological Neurons Olivary Nucleus |
| spellingShingle |
calcium channel calcium channel Cav2.1 calcium channel Cav3.1 calcium channel P type calcium channel Q type calcium channel T type unclassified drug calcium channel P type calcium channel Q type calcium channel T type animal cell animal experiment animal tissue article controlled study electric potential inferior olive mathematical model membrane potential mouse nerve cell nonhuman oscillation priority journal action potential amplitude Article cell function cellular parameters female inferior olive intracellular recording male membrane steady potential molecular imaging nerve cell membrane potential protein function protein transport signal transduction sinusoidal subthreshold membrane potential oscillation spike wave Animals Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, T-Type Calcium Signaling Computer Simulation Kinetics Membrane Potentials Mice Mice, Knockout Models, Neurological Neurons Olivary Nucleus Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| topic_facet |
calcium channel calcium channel Cav2.1 calcium channel Cav3.1 calcium channel P type calcium channel Q type calcium channel T type unclassified drug calcium channel P type calcium channel Q type calcium channel T type animal cell animal experiment animal tissue article controlled study electric potential inferior olive mathematical model membrane potential mouse nerve cell nonhuman oscillation priority journal action potential amplitude Article cell function cellular parameters female inferior olive intracellular recording male membrane steady potential molecular imaging nerve cell membrane potential protein function protein transport signal transduction sinusoidal subthreshold membrane potential oscillation spike wave Animals Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, T-Type Calcium Signaling Computer Simulation Kinetics Membrane Potentials Mice Mice, Knockout Models, Neurological Neurons Olivary Nucleus |
| description |
The role of P/Q- and T-type calcium channels in the rhythmic oscillatory behaviour of inferior olive (IO) neurons was investigated in mutant mice. Mice lacking either the CaV2.1 gene of the pore-forming α1A subunit for P/Q-type calcium channel, or the CaV3.1 gene of the pore-forming α1G subunit for T-type calcium channel were used. In vitro intracellular recording from IO neurons reveals that the amplitude and frequency of sinusoidal subthreshold oscillations (SSTOs) were reduced in the CaV2.1-/- mice. In the CaV3.1-/- mice, IO neurons also showed altered patterns of SSTOs and the probability of SSTO generation was significantly lower (15%, 5 of 34 neurons) than that of wild-type (78%, 31 of 40 neurons) or CaV2.1-/- mice (73%, 22 of 30 neurons). In addition, the low-threshold calcium spike and the sustained endogenous oscillation following rebound potentials were absent in IO neurons from CaV3.1-/- mice. Moreover, the phase-reset dynamics of oscillatory properties of single neurons and neuronal clusters in IO were remarkably altered in both CaV2.1-/- and CaV3.1-/- mice. These results suggest that both α1A P/Q- and α1G T-type calcium channels are required for the dynamic control of neuronal oscillations in the IO. These findings were supported by results from a mathematical IO neuronal model that incorporated T and P/Q channel kinetics. © 2010 The Authors. Journal compilation © 2010 The Physiological Society. |
| title |
Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| title_short |
Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| title_full |
Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| title_fullStr |
Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| title_full_unstemmed |
Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: A study in mutant mice |
| title_sort |
subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by p/q- and t-type calcium channels: a study in mutant mice |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v588_n16_p3031_Choi http://hdl.handle.net/20.500.12110/paper_00223751_v588_n16_p3031_Choi |
| _version_ |
1768542973683826688 |