Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast

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Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of th...

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Autores principales:	Ciancia, Marina, Alberghina, Josefina Silvia, Estevez, Jose Manuel
Publicado:	2012
Materias:	Bryopsis plumose Cell wall Green seaweed Pyruvylated galactan sulfate Sulfated polysaccharides Bryopsidaceae Bryopsis Bryopsis plumosa Caulerpales Chlorophyta Codium
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v48_n2_p326_Ciancia http://hdl.handle.net/20.500.12110/paper_00223646_v48_n2_p326_Ciancia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00223646_v48_n2_p326_Ciancia2023-06-08T14:50:08Z Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast Ciancia, Marina Alberghina, Josefina Silvia Estevez, Jose Manuel Bryopsis plumose Cell wall Green seaweed Pyruvylated galactan sulfate Sulfated polysaccharides Bryopsidaceae Bryopsis Bryopsis plumosa Caulerpales Chlorophyta Codium Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement. © 2012 Phycological Society of America. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alberghina, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v48_n2_p326_Ciancia http://hdl.handle.net/20.500.12110/paper_00223646_v48_n2_p326_Ciancia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bryopsis plumose Cell wall Green seaweed Pyruvylated galactan sulfate Sulfated polysaccharides Bryopsidaceae Bryopsis Bryopsis plumosa Caulerpales Chlorophyta Codium
spellingShingle	Bryopsis plumose Cell wall Green seaweed Pyruvylated galactan sulfate Sulfated polysaccharides Bryopsidaceae Bryopsis Bryopsis plumosa Caulerpales Chlorophyta Codium Ciancia, Marina Alberghina, Josefina Silvia Estevez, Jose Manuel Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
topic_facet	Bryopsis plumose Cell wall Green seaweed Pyruvylated galactan sulfate Sulfated polysaccharides Bryopsidaceae Bryopsis Bryopsis plumosa Caulerpales Chlorophyta Codium
description	Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement. © 2012 Phycological Society of America.
author	Ciancia, Marina Alberghina, Josefina Silvia Estevez, Jose Manuel
author_facet	Ciancia, Marina Alberghina, Josefina Silvia Estevez, Jose Manuel
author_sort	Ciancia, Marina
title	Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
title_short	Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
title_full	Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
title_fullStr	Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
title_full_unstemmed	Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
title_sort	characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v48_n2_p326_Ciancia http://hdl.handle.net/20.500.12110/paper_00223646_v48_n2_p326_Ciancia
work_keys_str_mv	AT cianciamarina characterizationofcellwallpolysaccharidesofthecoencocyticgreenseaweedbryopsisplumosabryopsidaceaechlorophytafromtheargentinecoast AT alberghinajosefinasilvia characterizationofcellwallpolysaccharidesofthecoencocyticgreenseaweedbryopsisplumosabryopsidaceaechlorophytafromtheargentinecoast AT estevezjosemanuel characterizationofcellwallpolysaccharidesofthecoencocyticgreenseaweedbryopsisplumosabryopsidaceaechlorophytafromtheargentinecoast
_version_	1768545271083433984

Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast

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