Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when...
Guardado en:
Autores principales: | , , |
---|---|
Publicado: |
2010
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v46_n3_p507_Martone http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone |
Aporte de: |
id |
paper:paper_00223646_v46_n3_p507_Martone |
---|---|
record_format |
dspace |
spelling |
paper:paper_00223646_v46_n3_p507_Martone2023-06-08T14:50:07Z Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) Navarro, Diego Alberto Stortz, Carlos Arturo Estevez, Jose Manuel Agarans Calliarthron cheilosporioides Cell wall Coralline algae Galactans Genicula Intergenicula Red seaweed algae Calliarthron cheilosporioides Corallinales Genicula Rhodophyta The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when calcified cells decalcify and restructure to create flexible tissue. The present study has identified important differences in the main agaran disaccharidic repeating units [→3)-β- d-Gal. p (1→ 4)-α- l-Gal. p(1→] synthesized by genicular and intergenicular segments. Based on chemical and spectroscopical analyses, we report that genicular cells from C. cheilosporioides biosynthesize a highly methoxylated galactan at C-6 position with low levels of branching with xylose side stubs on C-6 of the [→3)-β- d-Gal. p (1→] units, whereas intergenicular segments produce xylogalactans with high levels of xylose and low levels of 6- O-methyl β- d-Gal units. These data suggest that, during genicular development, xylosyl branched, 3-linked β- d-Gal. p units present in the xylogalactan backbones from intergenicular walls are mostly replaced by 6- O-methyl. -d-galactose units. We speculate that this structural shift is a consequence of a putative and specific methoxyl transferase that blocks the xylosylation on C-6 of the 3-linked β- d-Gal. p units. Changes in galactan substitutions may contribute to the distinct mechanical properties of genicula and may lend insight into the calcification process in coralline algae. © 2010 Phycological Society of America. Fil:Navarro, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v46_n3_p507_Martone http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Agarans Calliarthron cheilosporioides Cell wall Coralline algae Galactans Genicula Intergenicula Red seaweed algae Calliarthron cheilosporioides Corallinales Genicula Rhodophyta |
spellingShingle |
Agarans Calliarthron cheilosporioides Cell wall Coralline algae Galactans Genicula Intergenicula Red seaweed algae Calliarthron cheilosporioides Corallinales Genicula Rhodophyta Navarro, Diego Alberto Stortz, Carlos Arturo Estevez, Jose Manuel Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
topic_facet |
Agarans Calliarthron cheilosporioides Cell wall Coralline algae Galactans Genicula Intergenicula Red seaweed algae Calliarthron cheilosporioides Corallinales Genicula Rhodophyta |
description |
The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when calcified cells decalcify and restructure to create flexible tissue. The present study has identified important differences in the main agaran disaccharidic repeating units [→3)-β- d-Gal. p (1→ 4)-α- l-Gal. p(1→] synthesized by genicular and intergenicular segments. Based on chemical and spectroscopical analyses, we report that genicular cells from C. cheilosporioides biosynthesize a highly methoxylated galactan at C-6 position with low levels of branching with xylose side stubs on C-6 of the [→3)-β- d-Gal. p (1→] units, whereas intergenicular segments produce xylogalactans with high levels of xylose and low levels of 6- O-methyl β- d-Gal units. These data suggest that, during genicular development, xylosyl branched, 3-linked β- d-Gal. p units present in the xylogalactan backbones from intergenicular walls are mostly replaced by 6- O-methyl. -d-galactose units. We speculate that this structural shift is a consequence of a putative and specific methoxyl transferase that blocks the xylosylation on C-6 of the 3-linked β- d-Gal. p units. Changes in galactan substitutions may contribute to the distinct mechanical properties of genicula and may lend insight into the calcification process in coralline algae. © 2010 Phycological Society of America. |
author |
Navarro, Diego Alberto Stortz, Carlos Arturo Estevez, Jose Manuel |
author_facet |
Navarro, Diego Alberto Stortz, Carlos Arturo Estevez, Jose Manuel |
author_sort |
Navarro, Diego Alberto |
title |
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
title_short |
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
title_full |
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
title_fullStr |
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
title_full_unstemmed |
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
title_sort |
differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) |
publishDate |
2010 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v46_n3_p507_Martone http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone |
work_keys_str_mv |
AT navarrodiegoalberto differencesinpolysaccharidestructurebetweencalcifiedanduncalcifiedsegmentsinthecorallinecalliarthroncheilosporioidescorallinalesrhodophyta AT stortzcarlosarturo differencesinpolysaccharidestructurebetweencalcifiedanduncalcifiedsegmentsinthecorallinecalliarthroncheilosporioidescorallinalesrhodophyta AT estevezjosemanuel differencesinpolysaccharidestructurebetweencalcifiedanduncalcifiedsegmentsinthecorallinecalliarthroncheilosporioidescorallinalesrhodophyta |
_version_ |
1768544442796474368 |