Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina
Species of Ribeiroia use planorbid snails as intermediate host. Since there is little information about these digenean parasites in South America, we aimed to assess whether Ribeiroia cercariae from 3 north Argentina locations belonged to the same species and differed from Ribeiroia cercariae descri...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223395_v101_n5_p549_Davies http://hdl.handle.net/20.500.12110/paper_00223395_v101_n5_p549_Davies |
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paper:paper_00223395_v101_n5_p549_Davies2023-06-08T14:49:52Z Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina internal transcribed spacer 2 helminth DNA ribosomal spacer DNA ribosome RNA common ancestry database divergence flatworm genetic analysis genetic marker host range molecular ecology morphology snail Argentina Article Biomphalaria Biomphalaria occidentalis Biomphalaria orbignyi Biomphalaria tenagophila body height cercaria chemical structure controlled study intermediate host larval stage morphological trait nonhuman Ribeiroia cercariae Ribeiroia marini Ribeiroia ondatrae analysis of variance anatomy and histology animal Biomphalaria cercaria chemistry classification discriminant analysis disease carrier Echinostomatidae genetics isolation and purification nucleotide sequence parasitology phylogeny principal component analysis Argentina Biomphalaria Biomphalaria occidentalis Biomphalaria tenagophila Cercaria lileta Gastropoda Planorbidae Ribeiroia Ribeiroia marini Ribeiroia ondatrae Analysis of Variance Animals Argentina Base Sequence Biomphalaria Cercaria Discriminant Analysis Disease Vectors DNA, Helminth DNA, Ribosomal Spacer Echinostomatidae Phylogeny Principal Component Analysis RNA, Ribosomal Species of Ribeiroia use planorbid snails as intermediate host. Since there is little information about these digenean parasites in South America, we aimed to assess whether Ribeiroia cercariae from 3 north Argentina locations belonged to the same species and differed from Ribeiroia cercariae described elsewhere. Specimens were obtained from Biomphalaria tenagophila and Biomphalaria orbignyi (Salta Province), and Biomphalaria occidentalis (Corrientes Province). Morphological traits of cercariae were analyzed, as well as their sequence of the ribosomal internal transcribed spacer 2 (ITS2). The ITS2 region consisted of 426 nucleotides identical in all samples, suggesting that all specimens belong to the same species in spite of their morphological differences and first intermediate host species. Comparison of the ITS2 region with GenBank database records showed that specimens from Argentina were different from Ribeiroia ondatrae (0.9% divergence), Ribeiroia marini (0.7% divergence), and Cercaria lileta (0.2% divergence). In summary, morphological, ecological, and ITS2 molecular data suggest that specimens from Argentina belong to a different species. © 2015 American Society of Parasitologists. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223395_v101_n5_p549_Davies http://hdl.handle.net/20.500.12110/paper_00223395_v101_n5_p549_Davies |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
internal transcribed spacer 2 helminth DNA ribosomal spacer DNA ribosome RNA common ancestry database divergence flatworm genetic analysis genetic marker host range molecular ecology morphology snail Argentina Article Biomphalaria Biomphalaria occidentalis Biomphalaria orbignyi Biomphalaria tenagophila body height cercaria chemical structure controlled study intermediate host larval stage morphological trait nonhuman Ribeiroia cercariae Ribeiroia marini Ribeiroia ondatrae analysis of variance anatomy and histology animal Biomphalaria cercaria chemistry classification discriminant analysis disease carrier Echinostomatidae genetics isolation and purification nucleotide sequence parasitology phylogeny principal component analysis Argentina Biomphalaria Biomphalaria occidentalis Biomphalaria tenagophila Cercaria lileta Gastropoda Planorbidae Ribeiroia Ribeiroia marini Ribeiroia ondatrae Analysis of Variance Animals Argentina Base Sequence Biomphalaria Cercaria Discriminant Analysis Disease Vectors DNA, Helminth DNA, Ribosomal Spacer Echinostomatidae Phylogeny Principal Component Analysis RNA, Ribosomal |
| spellingShingle |
internal transcribed spacer 2 helminth DNA ribosomal spacer DNA ribosome RNA common ancestry database divergence flatworm genetic analysis genetic marker host range molecular ecology morphology snail Argentina Article Biomphalaria Biomphalaria occidentalis Biomphalaria orbignyi Biomphalaria tenagophila body height cercaria chemical structure controlled study intermediate host larval stage morphological trait nonhuman Ribeiroia cercariae Ribeiroia marini Ribeiroia ondatrae analysis of variance anatomy and histology animal Biomphalaria cercaria chemistry classification discriminant analysis disease carrier Echinostomatidae genetics isolation and purification nucleotide sequence parasitology phylogeny principal component analysis Argentina Biomphalaria Biomphalaria occidentalis Biomphalaria tenagophila Cercaria lileta Gastropoda Planorbidae Ribeiroia Ribeiroia marini Ribeiroia ondatrae Analysis of Variance Animals Argentina Base Sequence Biomphalaria Cercaria Discriminant Analysis Disease Vectors DNA, Helminth DNA, Ribosomal Spacer Echinostomatidae Phylogeny Principal Component Analysis RNA, Ribosomal Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| topic_facet |
internal transcribed spacer 2 helminth DNA ribosomal spacer DNA ribosome RNA common ancestry database divergence flatworm genetic analysis genetic marker host range molecular ecology morphology snail Argentina Article Biomphalaria Biomphalaria occidentalis Biomphalaria orbignyi Biomphalaria tenagophila body height cercaria chemical structure controlled study intermediate host larval stage morphological trait nonhuman Ribeiroia cercariae Ribeiroia marini Ribeiroia ondatrae analysis of variance anatomy and histology animal Biomphalaria cercaria chemistry classification discriminant analysis disease carrier Echinostomatidae genetics isolation and purification nucleotide sequence parasitology phylogeny principal component analysis Argentina Biomphalaria Biomphalaria occidentalis Biomphalaria tenagophila Cercaria lileta Gastropoda Planorbidae Ribeiroia Ribeiroia marini Ribeiroia ondatrae Analysis of Variance Animals Argentina Base Sequence Biomphalaria Cercaria Discriminant Analysis Disease Vectors DNA, Helminth DNA, Ribosomal Spacer Echinostomatidae Phylogeny Principal Component Analysis RNA, Ribosomal |
| description |
Species of Ribeiroia use planorbid snails as intermediate host. Since there is little information about these digenean parasites in South America, we aimed to assess whether Ribeiroia cercariae from 3 north Argentina locations belonged to the same species and differed from Ribeiroia cercariae described elsewhere. Specimens were obtained from Biomphalaria tenagophila and Biomphalaria orbignyi (Salta Province), and Biomphalaria occidentalis (Corrientes Province). Morphological traits of cercariae were analyzed, as well as their sequence of the ribosomal internal transcribed spacer 2 (ITS2). The ITS2 region consisted of 426 nucleotides identical in all samples, suggesting that all specimens belong to the same species in spite of their morphological differences and first intermediate host species. Comparison of the ITS2 region with GenBank database records showed that specimens from Argentina were different from Ribeiroia ondatrae (0.9% divergence), Ribeiroia marini (0.7% divergence), and Cercaria lileta (0.2% divergence). In summary, morphological, ecological, and ITS2 molecular data suggest that specimens from Argentina belong to a different species. © 2015 American Society of Parasitologists. |
| title |
Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| title_short |
Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| title_full |
Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| title_fullStr |
Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| title_full_unstemmed |
Morphological and ITS2 Molecular Characterization of Ribeiroia Cercariae (Digenea: Psilostomidae) from Biomphalaria spp. (Gastropoda: Planorbidae) in Northern Argentina |
| title_sort |
morphological and its2 molecular characterization of ribeiroia cercariae (digenea: psilostomidae) from biomphalaria spp. (gastropoda: planorbidae) in northern argentina |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223395_v101_n5_p549_Davies http://hdl.handle.net/20.500.12110/paper_00223395_v101_n5_p549_Davies |
| _version_ |
1768542208702545920 |