Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors
Serotonin receptors are targets of drug therapies for a variety of neuropsychiatric and neurodegenerative disorders. Cocaine inhibits the re-uptake of serotonin (5-HT), dopamine, and noradrenaline, whereas caffeine blocks adenosine receptors and opens ryanodine receptors in the endoplasmic reticulum...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223042_v136_n3_p526_Goitia http://hdl.handle.net/20.500.12110/paper_00223042_v136_n3_p526_Goitia |
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paper:paper_00223042_v136_n3_p526_Goitia |
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dspace |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
caffeine cocaine GABA serotonin thalamic reticular nucleus 1 (2 methoxyphenyl) 4 (4 phthalimidobutyl)piperazine adenosine caffeine cocaine serotonin serotonin 1A receptor serotonin 2A receptor serotonin receptor 4 aminobutyric acid cadmium chloride caffeine cocaine dopamine uptake inhibitor inositol 1,4,5 trisphosphate phosphodiesterase inhibitor phospholipase C serotonin serotonin 2A receptor serotonin receptor affecting agent 4 aminobutyric acid release animal experiment animal tissue Article concentration (parameters) controlled study excitatory postsynaptic potential GABAergic transmission inhibition kinetics male mouse neuromodulation nonhuman pharmacological blocking priority journal serotoninergic system thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp action potential animal dose response drug effects genetics in vitro study knockout mouse metabolism patch clamp technique thalamus nucleus Action Potentials Animals Cadmium Chloride Caffeine Cocaine Dopamine Uptake Inhibitors Dose-Response Relationship, Drug gamma-Aminobutyric Acid In Vitro Techniques Inositol 1,4,5-Trisphosphate Male Mice Mice, Knockout Patch-Clamp Techniques Phosphodiesterase Inhibitors Receptor, Serotonin, 5-HT2A Serotonin Serotonin Agents Thalamic Nuclei Type C Phospholipases |
| spellingShingle |
caffeine cocaine GABA serotonin thalamic reticular nucleus 1 (2 methoxyphenyl) 4 (4 phthalimidobutyl)piperazine adenosine caffeine cocaine serotonin serotonin 1A receptor serotonin 2A receptor serotonin receptor 4 aminobutyric acid cadmium chloride caffeine cocaine dopamine uptake inhibitor inositol 1,4,5 trisphosphate phosphodiesterase inhibitor phospholipase C serotonin serotonin 2A receptor serotonin receptor affecting agent 4 aminobutyric acid release animal experiment animal tissue Article concentration (parameters) controlled study excitatory postsynaptic potential GABAergic transmission inhibition kinetics male mouse neuromodulation nonhuman pharmacological blocking priority journal serotoninergic system thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp action potential animal dose response drug effects genetics in vitro study knockout mouse metabolism patch clamp technique thalamus nucleus Action Potentials Animals Cadmium Chloride Caffeine Cocaine Dopamine Uptake Inhibitors Dose-Response Relationship, Drug gamma-Aminobutyric Acid In Vitro Techniques Inositol 1,4,5-Trisphosphate Male Mice Mice, Knockout Patch-Clamp Techniques Phosphodiesterase Inhibitors Receptor, Serotonin, 5-HT2A Serotonin Serotonin Agents Thalamic Nuclei Type C Phospholipases Goitia, Belen Weisstaub, Noelia V. Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| topic_facet |
caffeine cocaine GABA serotonin thalamic reticular nucleus 1 (2 methoxyphenyl) 4 (4 phthalimidobutyl)piperazine adenosine caffeine cocaine serotonin serotonin 1A receptor serotonin 2A receptor serotonin receptor 4 aminobutyric acid cadmium chloride caffeine cocaine dopamine uptake inhibitor inositol 1,4,5 trisphosphate phosphodiesterase inhibitor phospholipase C serotonin serotonin 2A receptor serotonin receptor affecting agent 4 aminobutyric acid release animal experiment animal tissue Article concentration (parameters) controlled study excitatory postsynaptic potential GABAergic transmission inhibition kinetics male mouse neuromodulation nonhuman pharmacological blocking priority journal serotoninergic system thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp action potential animal dose response drug effects genetics in vitro study knockout mouse metabolism patch clamp technique thalamus nucleus Action Potentials Animals Cadmium Chloride Caffeine Cocaine Dopamine Uptake Inhibitors Dose-Response Relationship, Drug gamma-Aminobutyric Acid In Vitro Techniques Inositol 1,4,5-Trisphosphate Male Mice Mice, Knockout Patch-Clamp Techniques Phosphodiesterase Inhibitors Receptor, Serotonin, 5-HT2A Serotonin Serotonin Agents Thalamic Nuclei Type C Phospholipases |
| description |
Serotonin receptors are targets of drug therapies for a variety of neuropsychiatric and neurodegenerative disorders. Cocaine inhibits the re-uptake of serotonin (5-HT), dopamine, and noradrenaline, whereas caffeine blocks adenosine receptors and opens ryanodine receptors in the endoplasmic reticulum. We studied how 5-HT and adenosine affected spontaneous GABAergic transmission from thalamic reticular nucleus. We combined whole-cell patch clamp recordings of miniature inhibitory post-synaptic currents (mIPSCs) in ventrobasal thalamic neurons during local (puff) application of 5-HT in wild type (WT) or knockout mice lacking 5-HT 2A receptors (5-HT 2A -/-). Inhibition of mIPSCs frequency by low (10 μM) and high (100 μM) 5-HT concentrations was observed in ventrobasal neurons from 5-HT 2A -/- mice. In WT mice, only 100 μM 5-HT significantly reduced mIPSCs frequency. In 5-HT 2A -/- mice, NAN-190, a specific 5-HT 1A antagonist, prevented the 100 μM 5-HT inhibition while blocking H-currents that prolonged inhibition during post-puff periods. The inhibitory effects of 100 μM 5-HT were enhanced in cocaine binge-treated 5-HT 2A -/- mice. Caffeine binge treatment did not affect 5-HT-mediated inhibition. Our findings suggest that both 5-HT 1A and 5-HT 2A receptors are present in pre-synaptic thalamic reticular nucleus terminals. Serotonergic-mediated inhibition of GABA release could underlie aberrant thalamocortical physiology described after repetitive consumption of cocaine. Our findings suggest that both 5-HT 1A , 5-HT 2A and A1 receptors are present in pre-synaptic TRN terminals. 5-HT 1A and A1 receptors would down-regulate adenylate cyclase, whereas 5-HT 1A would also increase the probability of the opening of G-protein-activated inwardly rectifying K + channels (GIRK). Sustained opening of GIRK channels would hyperpolarize pre-synaptic terminals activating H-currents, resulting in less GABA release. 5-HT 2A -would activate PLC and IP 3 , increasing intracellular [Ca 2+ ] and thus facilitating GABA release. Our findings suggest that both 5-HT 1A , 5-HT 2A and A1 receptors are present in pre-synaptic TRN terminals. 5-HT 1A and A1 receptors would down-regulate adenylate cyclase, whereas 5-HT 1A would also increase the probability of the opening of G-protein-activated inwardly rectifying K + channels (GIRK). Sustained opening of GIRK channels would hyperpolarize pre-synaptic terminals activating H-currents, resulting in less GABA release. 5-HT 2A -would activate PLC and IP 3 , increasing intracellular [Ca 2+ ] and thus facilitating GABA release. © 2015 International Society for Neurochemistry. |
| author |
Goitia, Belen Weisstaub, Noelia V. |
| author_facet |
Goitia, Belen Weisstaub, Noelia V. |
| author_sort |
Goitia, Belen |
| title |
Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| title_short |
Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| title_full |
Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| title_fullStr |
Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| title_full_unstemmed |
Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors |
| title_sort |
modulation of gaba release from the thalamic reticular nucleus by cocaine and caffeine: role of serotonin receptors |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223042_v136_n3_p526_Goitia http://hdl.handle.net/20.500.12110/paper_00223042_v136_n3_p526_Goitia |
| work_keys_str_mv |
AT goitiabelen modulationofgabareleasefromthethalamicreticularnucleusbycocaineandcaffeineroleofserotoninreceptors AT weisstaubnoeliav modulationofgabareleasefromthethalamicreticularnucleusbycocaineandcaffeineroleofserotoninreceptors |
| _version_ |
1768546383107719168 |
| spelling |
paper:paper_00223042_v136_n3_p526_Goitia2023-06-08T14:49:06Z Modulation of GABA release from the thalamic reticular nucleus by cocaine and caffeine: Role of serotonin receptors Goitia, Belen Weisstaub, Noelia V. caffeine cocaine GABA serotonin thalamic reticular nucleus 1 (2 methoxyphenyl) 4 (4 phthalimidobutyl)piperazine adenosine caffeine cocaine serotonin serotonin 1A receptor serotonin 2A receptor serotonin receptor 4 aminobutyric acid cadmium chloride caffeine cocaine dopamine uptake inhibitor inositol 1,4,5 trisphosphate phosphodiesterase inhibitor phospholipase C serotonin serotonin 2A receptor serotonin receptor affecting agent 4 aminobutyric acid release animal experiment animal tissue Article concentration (parameters) controlled study excitatory postsynaptic potential GABAergic transmission inhibition kinetics male mouse neuromodulation nonhuman pharmacological blocking priority journal serotoninergic system thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp action potential animal dose response drug effects genetics in vitro study knockout mouse metabolism patch clamp technique thalamus nucleus Action Potentials Animals Cadmium Chloride Caffeine Cocaine Dopamine Uptake Inhibitors Dose-Response Relationship, Drug gamma-Aminobutyric Acid In Vitro Techniques Inositol 1,4,5-Trisphosphate Male Mice Mice, Knockout Patch-Clamp Techniques Phosphodiesterase Inhibitors Receptor, Serotonin, 5-HT2A Serotonin Serotonin Agents Thalamic Nuclei Type C Phospholipases Serotonin receptors are targets of drug therapies for a variety of neuropsychiatric and neurodegenerative disorders. Cocaine inhibits the re-uptake of serotonin (5-HT), dopamine, and noradrenaline, whereas caffeine blocks adenosine receptors and opens ryanodine receptors in the endoplasmic reticulum. We studied how 5-HT and adenosine affected spontaneous GABAergic transmission from thalamic reticular nucleus. We combined whole-cell patch clamp recordings of miniature inhibitory post-synaptic currents (mIPSCs) in ventrobasal thalamic neurons during local (puff) application of 5-HT in wild type (WT) or knockout mice lacking 5-HT 2A receptors (5-HT 2A -/-). Inhibition of mIPSCs frequency by low (10 μM) and high (100 μM) 5-HT concentrations was observed in ventrobasal neurons from 5-HT 2A -/- mice. In WT mice, only 100 μM 5-HT significantly reduced mIPSCs frequency. In 5-HT 2A -/- mice, NAN-190, a specific 5-HT 1A antagonist, prevented the 100 μM 5-HT inhibition while blocking H-currents that prolonged inhibition during post-puff periods. The inhibitory effects of 100 μM 5-HT were enhanced in cocaine binge-treated 5-HT 2A -/- mice. Caffeine binge treatment did not affect 5-HT-mediated inhibition. Our findings suggest that both 5-HT 1A and 5-HT 2A receptors are present in pre-synaptic thalamic reticular nucleus terminals. Serotonergic-mediated inhibition of GABA release could underlie aberrant thalamocortical physiology described after repetitive consumption of cocaine. Our findings suggest that both 5-HT 1A , 5-HT 2A and A1 receptors are present in pre-synaptic TRN terminals. 5-HT 1A and A1 receptors would down-regulate adenylate cyclase, whereas 5-HT 1A would also increase the probability of the opening of G-protein-activated inwardly rectifying K + channels (GIRK). Sustained opening of GIRK channels would hyperpolarize pre-synaptic terminals activating H-currents, resulting in less GABA release. 5-HT 2A -would activate PLC and IP 3 , increasing intracellular [Ca 2+ ] and thus facilitating GABA release. Our findings suggest that both 5-HT 1A , 5-HT 2A and A1 receptors are present in pre-synaptic TRN terminals. 5-HT 1A and A1 receptors would down-regulate adenylate cyclase, whereas 5-HT 1A would also increase the probability of the opening of G-protein-activated inwardly rectifying K + channels (GIRK). Sustained opening of GIRK channels would hyperpolarize pre-synaptic terminals activating H-currents, resulting in less GABA release. 5-HT 2A -would activate PLC and IP 3 , increasing intracellular [Ca 2+ ] and thus facilitating GABA release. © 2015 International Society for Neurochemistry. Fil:Goitia, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Weisstaub, N.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223042_v136_n3_p526_Goitia http://hdl.handle.net/20.500.12110/paper_00223042_v136_n3_p526_Goitia |