Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v58_n5_p557_Clemente http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente |
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paper:paper_00222844_v58_n5_p557_Clemente2023-06-08T14:48:47Z Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence Clemente, Marina Lia, Verónica Viviana Matrajt, Mariana Laura Angel, Sergio Oscar EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1 and B2. A search for internal repetitions within this family permitted the identification of conserved regions and the design of PCR primers that amplify almost all these repetitive elements. These primers amplified the expected 350-bp repeats and a novel 680-bp repetitive element (Sat680) related to this family. Two additional tandemly repeated high-order structures corresponding to this satellite DNA family were found by searching the Toxoplasma genome database with these sequences. These studies were confirmed by sequence analysis and identified: (1) an arrangement of AB1CB2 350-bp repeats and (2) an arrangement of two 350-bp-like repeats, resulting in a 680-bp monomer. Sequence comparison and phylogenetic analysis indicated that both high-order structures may have originated from the same ancestral 350-bp repeat. PCR amplification, sequence analysis and Southern blot showed that similar high-order structures were also found in the Toxoplasma-sister taxon Neospora caninum. The Toxoplasma genome database (http://ToxoDB.org) permitted the assembly of a contig harboring Sat350 elements at one end and a long nonrepetitive DNA sequence flanking this satellite DNA. The region bordering the Sat350 repeats contained two differentially expressed sequence-related regions and interstitial telomeric sequences. Fil:Clemente, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lia, V.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matrajt, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Angel, S.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v58_n5_p557_Clemente http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii |
| spellingShingle |
EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii Clemente, Marina Lia, Verónica Viviana Matrajt, Mariana Laura Angel, Sergio Oscar Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| topic_facet |
EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii |
| description |
A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1 and B2. A search for internal repetitions within this family permitted the identification of conserved regions and the design of PCR primers that amplify almost all these repetitive elements. These primers amplified the expected 350-bp repeats and a novel 680-bp repetitive element (Sat680) related to this family. Two additional tandemly repeated high-order structures corresponding to this satellite DNA family were found by searching the Toxoplasma genome database with these sequences. These studies were confirmed by sequence analysis and identified: (1) an arrangement of AB1CB2 350-bp repeats and (2) an arrangement of two 350-bp-like repeats, resulting in a 680-bp monomer. Sequence comparison and phylogenetic analysis indicated that both high-order structures may have originated from the same ancestral 350-bp repeat. PCR amplification, sequence analysis and Southern blot showed that similar high-order structures were also found in the Toxoplasma-sister taxon Neospora caninum. The Toxoplasma genome database (http://ToxoDB.org) permitted the assembly of a contig harboring Sat350 elements at one end and a long nonrepetitive DNA sequence flanking this satellite DNA. The region bordering the Sat350 repeats contained two differentially expressed sequence-related regions and interstitial telomeric sequences. |
| author |
Clemente, Marina Lia, Verónica Viviana Matrajt, Mariana Laura Angel, Sergio Oscar |
| author_facet |
Clemente, Marina Lia, Verónica Viviana Matrajt, Mariana Laura Angel, Sergio Oscar |
| author_sort |
Clemente, Marina |
| title |
Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| title_short |
Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| title_full |
Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| title_fullStr |
Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| title_full_unstemmed |
Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence |
| title_sort |
structure analysis of two toxoplasma gondii and neospora caninum satellite dna families and evolution of their common monomeric sequence |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v58_n5_p557_Clemente http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente |
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AT clementemarina structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT liaveronicaviviana structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT matrajtmarianalaura structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT angelsergiooscar structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence |
| _version_ |
1768544166074122240 |