Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age
The medfly Ceratitis capitata is an agricultural pest distributed worldwide thanks, in part, to its phenotypic plasticity of thermal tolerance. Cold exposure has been shown to reduce C. capitata survival, which may affect its distribution in areas with subfreezing temperatures. When insects are incr...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2014
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v71_n_p156_PujolLereis http://hdl.handle.net/20.500.12110/paper_00221910_v71_n_p156_PujolLereis |
| Aporte de: |
| id |
paper:paper_00221910_v71_n_p156_PujolLereis |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00221910_v71_n_p156_PujolLereis2023-06-08T14:47:17Z Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age Pujol Lereis, Luciana Mercedes Rabossi, Alejandro Quesada Allué, Luis Alberto Ceratitis capitata Chill-coma recovery Chilling-injury Population heterogeneity Superoxide dismutase Survival adaptation age antioxidant bioassay enzyme activity fly gene expression heterogeneity mortality pest species phenotypic plasticity physiology population structure survival temperature tolerance Ceratitis capitata heat shock protein heat shock protein 70 insect protein acclimatization age animal cold shock response female gene expression regulation genetics growth, development and aging longevity male Mediterranean fruit fly metabolism physiology sex difference Acclimatization Age Factors Animals Ceratitis capitata Cold-Shock Response Female Gene Expression Regulation Heat-Shock Proteins HSP70 Heat-Shock Proteins Insect Proteins Longevity Male Sex Factors The medfly Ceratitis capitata is an agricultural pest distributed worldwide thanks, in part, to its phenotypic plasticity of thermal tolerance. Cold exposure has been shown to reduce C. capitata survival, which may affect its distribution in areas with subfreezing temperatures. When insects are increasingly cooled, they attain a critical thermal threshold and enter a chill-coma state characterized by cessation of movement. It is not clear how a rapid cold exposure affects the physiological state of medflies, and how this is influenced by age and population heterogeneity. In order to approach these questions, C. capitata single-sex laboratory populations of 15 and 30. days old were subjected to a chill-coma recovery assay, and separated according to their recovery time in three subgroups: Fast-Subgroups, Intermediate-Subgroups, and Slow-Subgroups. Thereafter, we analyzed their survival, behavioral, and gene expression outputs. In female and old male populations, we found that flies with the slowest recovery time had a reduced life expectancy, a higher initial mortality rate, and a worse climbing performance compared with flies that recovered faster. Therefore, we were able to separate subgroups that developed chilling-injury from subgroups that had a reversible full recovery after cold exposure. The gene expression analysis of the heat shock protein genes hsp70 and hsp83 showed no clear association with the parameters studied. Interestingly, thorax expression levels of the Cu/Zn superoxide dismutase gene were elevated during the recovery phase in the Fast-Subgroups, but remained constant in the Slow-Subgroups that developed chilling-injury. On the other hand, none of the young male subgroups seemed to have suffered irreversible damage. Thus, we concluded that depending on age and population heterogeneity, chill-coma recovery time points out significant differences on individual cold tolerance. Moreover, the inability to properly induce the antioxidant defense system to counteract the oxidative damage caused by cold seems to contribute to the development of chilling-injury. © 2014 Elsevier Ltd. Fil:Pujol-Lereis, L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rabossi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v71_n_p156_PujolLereis http://hdl.handle.net/20.500.12110/paper_00221910_v71_n_p156_PujolLereis |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ceratitis capitata Chill-coma recovery Chilling-injury Population heterogeneity Superoxide dismutase Survival adaptation age antioxidant bioassay enzyme activity fly gene expression heterogeneity mortality pest species phenotypic plasticity physiology population structure survival temperature tolerance Ceratitis capitata heat shock protein heat shock protein 70 insect protein acclimatization age animal cold shock response female gene expression regulation genetics growth, development and aging longevity male Mediterranean fruit fly metabolism physiology sex difference Acclimatization Age Factors Animals Ceratitis capitata Cold-Shock Response Female Gene Expression Regulation Heat-Shock Proteins HSP70 Heat-Shock Proteins Insect Proteins Longevity Male Sex Factors |
| spellingShingle |
Ceratitis capitata Chill-coma recovery Chilling-injury Population heterogeneity Superoxide dismutase Survival adaptation age antioxidant bioassay enzyme activity fly gene expression heterogeneity mortality pest species phenotypic plasticity physiology population structure survival temperature tolerance Ceratitis capitata heat shock protein heat shock protein 70 insect protein acclimatization age animal cold shock response female gene expression regulation genetics growth, development and aging longevity male Mediterranean fruit fly metabolism physiology sex difference Acclimatization Age Factors Animals Ceratitis capitata Cold-Shock Response Female Gene Expression Regulation Heat-Shock Proteins HSP70 Heat-Shock Proteins Insect Proteins Longevity Male Sex Factors Pujol Lereis, Luciana Mercedes Rabossi, Alejandro Quesada Allué, Luis Alberto Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| topic_facet |
Ceratitis capitata Chill-coma recovery Chilling-injury Population heterogeneity Superoxide dismutase Survival adaptation age antioxidant bioassay enzyme activity fly gene expression heterogeneity mortality pest species phenotypic plasticity physiology population structure survival temperature tolerance Ceratitis capitata heat shock protein heat shock protein 70 insect protein acclimatization age animal cold shock response female gene expression regulation genetics growth, development and aging longevity male Mediterranean fruit fly metabolism physiology sex difference Acclimatization Age Factors Animals Ceratitis capitata Cold-Shock Response Female Gene Expression Regulation Heat-Shock Proteins HSP70 Heat-Shock Proteins Insect Proteins Longevity Male Sex Factors |
| description |
The medfly Ceratitis capitata is an agricultural pest distributed worldwide thanks, in part, to its phenotypic plasticity of thermal tolerance. Cold exposure has been shown to reduce C. capitata survival, which may affect its distribution in areas with subfreezing temperatures. When insects are increasingly cooled, they attain a critical thermal threshold and enter a chill-coma state characterized by cessation of movement. It is not clear how a rapid cold exposure affects the physiological state of medflies, and how this is influenced by age and population heterogeneity. In order to approach these questions, C. capitata single-sex laboratory populations of 15 and 30. days old were subjected to a chill-coma recovery assay, and separated according to their recovery time in three subgroups: Fast-Subgroups, Intermediate-Subgroups, and Slow-Subgroups. Thereafter, we analyzed their survival, behavioral, and gene expression outputs. In female and old male populations, we found that flies with the slowest recovery time had a reduced life expectancy, a higher initial mortality rate, and a worse climbing performance compared with flies that recovered faster. Therefore, we were able to separate subgroups that developed chilling-injury from subgroups that had a reversible full recovery after cold exposure. The gene expression analysis of the heat shock protein genes hsp70 and hsp83 showed no clear association with the parameters studied. Interestingly, thorax expression levels of the Cu/Zn superoxide dismutase gene were elevated during the recovery phase in the Fast-Subgroups, but remained constant in the Slow-Subgroups that developed chilling-injury. On the other hand, none of the young male subgroups seemed to have suffered irreversible damage. Thus, we concluded that depending on age and population heterogeneity, chill-coma recovery time points out significant differences on individual cold tolerance. Moreover, the inability to properly induce the antioxidant defense system to counteract the oxidative damage caused by cold seems to contribute to the development of chilling-injury. © 2014 Elsevier Ltd. |
| author |
Pujol Lereis, Luciana Mercedes Rabossi, Alejandro Quesada Allué, Luis Alberto |
| author_facet |
Pujol Lereis, Luciana Mercedes Rabossi, Alejandro Quesada Allué, Luis Alberto |
| author_sort |
Pujol Lereis, Luciana Mercedes |
| title |
Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| title_short |
Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| title_full |
Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| title_fullStr |
Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| title_full_unstemmed |
Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age |
| title_sort |
analysis of survival, gene expression and behavior following chill-coma in the medfly ceratitis capitata: effects of population heterogeneity and age |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v71_n_p156_PujolLereis http://hdl.handle.net/20.500.12110/paper_00221910_v71_n_p156_PujolLereis |
| work_keys_str_mv |
AT pujollereislucianamercedes analysisofsurvivalgeneexpressionandbehaviorfollowingchillcomainthemedflyceratitiscapitataeffectsofpopulationheterogeneityandage AT rabossialejandro analysisofsurvivalgeneexpressionandbehaviorfollowingchillcomainthemedflyceratitiscapitataeffectsofpopulationheterogeneityandage AT quesadaallueluisalberto analysisofsurvivalgeneexpressionandbehaviorfollowingchillcomainthemedflyceratitiscapitataeffectsofpopulationheterogeneityandage |
| _version_ |
1768545725269934080 |