Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster

Knockdown resistance to high temperature (KRHT) is a thermal adaptation trait in Drosophila melanogaster. Here we used quantitative real-time PCR (qRT-PCR) to test for possible associations between KRHT and the expression of candidate genes within quantitative trait loci (QTL) in eight recombinant i...

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Autor principal:	Norry, Fabian Marcelo
Publicado:	2009
Materias:	Gene expression Principal Components Heat knockdown resistance Quantitative polymerase chain reaction Quantitative trait loci Recombinant inbred lines Drosophila protein chromosome fly gene expression polymerase chain reaction temperature tolerance animal article chemistry Drosophila melanogaster female gene expression regulation genetics heat male metabolism quantitative trait locus Animals Drosophila Proteins Female Gene Expression Regulation Hot Temperature Male Quantitative Trait Loci
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v55_n11_p1050_Norry http://hdl.handle.net/20.500.12110/paper_00221910_v55_n11_p1050_Norry
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00221910_v55_n11_p1050_Norry
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spelling	paper:paper_00221910_v55_n11_p1050_Norry2023-06-08T14:47:12Z Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster Norry, Fabian Marcelo Gene expression Principal Components Heat knockdown resistance Quantitative polymerase chain reaction Quantitative trait loci Recombinant inbred lines Drosophila protein chromosome fly gene expression polymerase chain reaction temperature tolerance animal article chemistry Drosophila melanogaster female gene expression regulation genetics heat male metabolism quantitative trait locus Animals Drosophila melanogaster Drosophila Proteins Female Gene Expression Regulation Hot Temperature Male Quantitative Trait Loci Drosophila melanogaster Knockdown resistance to high temperature (KRHT) is a thermal adaptation trait in Drosophila melanogaster. Here we used quantitative real-time PCR (qRT-PCR) to test for possible associations between KRHT and the expression of candidate genes within quantitative trait loci (QTL) in eight recombinant inbred lines (RIL). hsp60 and hsc70-3 map within an X-linked QTL, while CG10383, catsup, ddc, trap1, and cyp6a13 are linked in a KRHT-QTL on chromosome 2. hsc70-3 expression increased by heat-hardening. Principal Components analysis revealed that catsup, ddc and trap1 were either co-expressed or combined in their expression levels. This composite expression variable (e-PC1) was positively associated to KRHT in non-hardened RIL. In heat-hardened flies, hsp60 was negatively related to hsc70-3 on e-PC2, with effects on KRHT. These results are consistent with the notion that QTL can be shaped by expression variation in combined candidate loci. We found composite variables of gene expression (e-PCs) that best correlated to KRHT. Network effects with other untested linked loci are apparent because, in spite of their associations with KRHT phenotypes, e-PCs were sometimes uncorrelated with their QTL genotype. © 2009 Elsevier Ltd. All rights reserved. Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v55_n11_p1050_Norry http://hdl.handle.net/20.500.12110/paper_00221910_v55_n11_p1050_Norry
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Gene expression Principal Components Heat knockdown resistance Quantitative polymerase chain reaction Quantitative trait loci Recombinant inbred lines Drosophila protein chromosome fly gene expression polymerase chain reaction temperature tolerance animal article chemistry Drosophila melanogaster female gene expression regulation genetics heat male metabolism quantitative trait locus Animals Drosophila melanogaster Drosophila Proteins Female Gene Expression Regulation Hot Temperature Male Quantitative Trait Loci Drosophila melanogaster
spellingShingle	Gene expression Principal Components Heat knockdown resistance Quantitative polymerase chain reaction Quantitative trait loci Recombinant inbred lines Drosophila protein chromosome fly gene expression polymerase chain reaction temperature tolerance animal article chemistry Drosophila melanogaster female gene expression regulation genetics heat male metabolism quantitative trait locus Animals Drosophila melanogaster Drosophila Proteins Female Gene Expression Regulation Hot Temperature Male Quantitative Trait Loci Drosophila melanogaster Norry, Fabian Marcelo Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
topic_facet	Gene expression Principal Components Heat knockdown resistance Quantitative polymerase chain reaction Quantitative trait loci Recombinant inbred lines Drosophila protein chromosome fly gene expression polymerase chain reaction temperature tolerance animal article chemistry Drosophila melanogaster female gene expression regulation genetics heat male metabolism quantitative trait locus Animals Drosophila melanogaster Drosophila Proteins Female Gene Expression Regulation Hot Temperature Male Quantitative Trait Loci Drosophila melanogaster
description	Knockdown resistance to high temperature (KRHT) is a thermal adaptation trait in Drosophila melanogaster. Here we used quantitative real-time PCR (qRT-PCR) to test for possible associations between KRHT and the expression of candidate genes within quantitative trait loci (QTL) in eight recombinant inbred lines (RIL). hsp60 and hsc70-3 map within an X-linked QTL, while CG10383, catsup, ddc, trap1, and cyp6a13 are linked in a KRHT-QTL on chromosome 2. hsc70-3 expression increased by heat-hardening. Principal Components analysis revealed that catsup, ddc and trap1 were either co-expressed or combined in their expression levels. This composite expression variable (e-PC1) was positively associated to KRHT in non-hardened RIL. In heat-hardened flies, hsp60 was negatively related to hsc70-3 on e-PC2, with effects on KRHT. These results are consistent with the notion that QTL can be shaped by expression variation in combined candidate loci. We found composite variables of gene expression (e-PCs) that best correlated to KRHT. Network effects with other untested linked loci are apparent because, in spite of their associations with KRHT phenotypes, e-PCs were sometimes uncorrelated with their QTL genotype. © 2009 Elsevier Ltd. All rights reserved.
author	Norry, Fabian Marcelo
author_facet	Norry, Fabian Marcelo
author_sort	Norry, Fabian Marcelo
title	Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
title_short	Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
title_full	Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
title_fullStr	Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
title_full_unstemmed	Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
title_sort	combined expression patterns of qtl-linked candidate genes best predict thermotolerance in drosophila melanogaster
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v55_n11_p1050_Norry http://hdl.handle.net/20.500.12110/paper_00221910_v55_n11_p1050_Norry
work_keys_str_mv	AT norryfabianmarcelo combinedexpressionpatternsofqtllinkedcandidategenesbestpredictthermotoleranceindrosophilamelanogaster
_version_	1768543978310860800

Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster

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