Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v184_n11_p6386_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass |
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paper:paper_00221767_v184_n11_p6386_FuxmanBass2023-06-08T14:46:57Z Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms Fuxman Bass, Juan Ignacio Russo, Daniela Marta Gabelloni, María Laura Giordano, Mirta Nilda Zorreguieta, Angeles Trevani, Analía S. CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc. Fil:Fuxman Bass, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Russo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabelloni, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, Á. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Trevani, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v184_n11_p6386_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa |
| spellingShingle |
CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa Fuxman Bass, Juan Ignacio Russo, Daniela Marta Gabelloni, María Laura Giordano, Mirta Nilda Zorreguieta, Angeles Trevani, Analía S. Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| topic_facet |
CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa |
| description |
We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc. |
| author |
Fuxman Bass, Juan Ignacio Russo, Daniela Marta Gabelloni, María Laura Giordano, Mirta Nilda Zorreguieta, Angeles Trevani, Analía S. |
| author_facet |
Fuxman Bass, Juan Ignacio Russo, Daniela Marta Gabelloni, María Laura Giordano, Mirta Nilda Zorreguieta, Angeles Trevani, Analía S. |
| author_sort |
Fuxman Bass, Juan Ignacio |
| title |
Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| title_short |
Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| title_full |
Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| title_fullStr |
Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| title_full_unstemmed |
Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms |
| title_sort |
extracellular dna: a major proinflammatory component of pseudomonas aeruginosa biofilms |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v184_n11_p6386_FuxmanBass http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass |
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| _version_ |
1768543019998380032 |