Persistent infection of Vero cells with Tacaribe virus
Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v56_n1_p41_Damonte http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte |
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paper:paper_00221317_v56_n1_p41_Damonte2025-07-30T17:28:42Z Persistent infection of Vero cells with Tacaribe virus animal experiment cell culture in vitro study persistent infection tacaribe arenavirus Animalia Arenavirus Tacaribe virus Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6 passages, a cyclical pattern was observed between passages 6 and 16, and subsequently no virus infectivity could be detected. Co-cultivation with normal RK-13 or Vero cells enhanced virus yield from virus-producing cultures of Veri-T cells (passage 15), but the addition of susceptible cells had no effect on non-producer Vero-T cultures (passage 19). Only a small proportion (<1%) of the persistently infected cells tested during the first 16 passages produced infectious virus. The virus released during the early stages of persistence was temperature-sensitive if grown at 40°C, more thermolabile at 50°C than parental virus, and unable to initiate a persistent infection in Vero cells. Vero-T cells consistently showed refractoriness to homotypic Tacaribe virus superinfection and a selective graded resistance to other arenavirus replication. The possible use of viral susceptibility of persistently infected cultures as marker of antigenic relationship among Tacaribe complex viruses is considered. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v56_n1_p41_Damonte http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal experiment cell culture in vitro study persistent infection tacaribe arenavirus Animalia Arenavirus Tacaribe virus |
| spellingShingle |
animal experiment cell culture in vitro study persistent infection tacaribe arenavirus Animalia Arenavirus Tacaribe virus Persistent infection of Vero cells with Tacaribe virus |
| topic_facet |
animal experiment cell culture in vitro study persistent infection tacaribe arenavirus Animalia Arenavirus Tacaribe virus |
| description |
Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6 passages, a cyclical pattern was observed between passages 6 and 16, and subsequently no virus infectivity could be detected. Co-cultivation with normal RK-13 or Vero cells enhanced virus yield from virus-producing cultures of Veri-T cells (passage 15), but the addition of susceptible cells had no effect on non-producer Vero-T cultures (passage 19). Only a small proportion (<1%) of the persistently infected cells tested during the first 16 passages produced infectious virus. The virus released during the early stages of persistence was temperature-sensitive if grown at 40°C, more thermolabile at 50°C than parental virus, and unable to initiate a persistent infection in Vero cells. Vero-T cells consistently showed refractoriness to homotypic Tacaribe virus superinfection and a selective graded resistance to other arenavirus replication. The possible use of viral susceptibility of persistently infected cultures as marker of antigenic relationship among Tacaribe complex viruses is considered. |
| title |
Persistent infection of Vero cells with Tacaribe virus |
| title_short |
Persistent infection of Vero cells with Tacaribe virus |
| title_full |
Persistent infection of Vero cells with Tacaribe virus |
| title_fullStr |
Persistent infection of Vero cells with Tacaribe virus |
| title_full_unstemmed |
Persistent infection of Vero cells with Tacaribe virus |
| title_sort |
persistent infection of vero cells with tacaribe virus |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v56_n1_p41_Damonte http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte |
| _version_ |
1840321602898624512 |