In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations
Acetobacter xylinum strain NRRL B42 and its derivative RCGr1 produce a complex exopolysaccharide, acetan, containing glucose, mannose, glucuronic acid and rhamnose in a 4:1:1:1 molar ratio. The in vitro synthesis of acetan, employing electroporated cells as the enzyme system and the respective 14C-l...
Guardado en:
| Publicado: |
1993
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221287_v139_n11_p2745_Semino http://hdl.handle.net/20.500.12110/paper_00221287_v139_n11_p2745_Semino |
| Aporte de: |
| id |
paper:paper_00221287_v139_n11_p2745_Semino |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00221287_v139_n11_p2745_Semino2023-06-08T14:46:30Z In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations acetic acid derivative acyl coenzyme A carbon 14 edetic acid enzyme glucan glucose glucuronic acid mannose oligosaccharide polysaccharide prenylamine rhamnose sugar nucleotide Acetobacter article carbohydrate synthesis electroporation gel filtration isotope labeling methylation molecular weight nonhuman precursor priority journal Acetobacter xylinum strain NRRL B42 and its derivative RCGr1 produce a complex exopolysaccharide, acetan, containing glucose, mannose, glucuronic acid and rhamnose in a 4:1:1:1 molar ratio. The in vitro synthesis of acetan, employing electroporated cells as the enzyme system and the respective 14C-labelled sugar nucleotide precursors, is described. The synthesis of the prenyl-linked heptasaccharide repeat unit, already observed in EDTA-treated cells, was confirmed, as well as the formation of other saccharides not related to acetan biosynthesis, including a high molecular mass glucan. The acetan formed was characterized by gel filtration, specific radioactive labelling with each precursor and permethylation analysis. It was also shown that acetan contains acetyl residues and that using [14C]acetyl CoA as donor, radioactivity was detected both at the polysaccharide and at the prenyl-linked oligosaccharide stage. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221287_v139_n11_p2745_Semino http://hdl.handle.net/20.500.12110/paper_00221287_v139_n11_p2745_Semino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetic acid derivative acyl coenzyme A carbon 14 edetic acid enzyme glucan glucose glucuronic acid mannose oligosaccharide polysaccharide prenylamine rhamnose sugar nucleotide Acetobacter article carbohydrate synthesis electroporation gel filtration isotope labeling methylation molecular weight nonhuman precursor priority journal |
| spellingShingle |
acetic acid derivative acyl coenzyme A carbon 14 edetic acid enzyme glucan glucose glucuronic acid mannose oligosaccharide polysaccharide prenylamine rhamnose sugar nucleotide Acetobacter article carbohydrate synthesis electroporation gel filtration isotope labeling methylation molecular weight nonhuman precursor priority journal In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| topic_facet |
acetic acid derivative acyl coenzyme A carbon 14 edetic acid enzyme glucan glucose glucuronic acid mannose oligosaccharide polysaccharide prenylamine rhamnose sugar nucleotide Acetobacter article carbohydrate synthesis electroporation gel filtration isotope labeling methylation molecular weight nonhuman precursor priority journal |
| description |
Acetobacter xylinum strain NRRL B42 and its derivative RCGr1 produce a complex exopolysaccharide, acetan, containing glucose, mannose, glucuronic acid and rhamnose in a 4:1:1:1 molar ratio. The in vitro synthesis of acetan, employing electroporated cells as the enzyme system and the respective 14C-labelled sugar nucleotide precursors, is described. The synthesis of the prenyl-linked heptasaccharide repeat unit, already observed in EDTA-treated cells, was confirmed, as well as the formation of other saccharides not related to acetan biosynthesis, including a high molecular mass glucan. The acetan formed was characterized by gel filtration, specific radioactive labelling with each precursor and permethylation analysis. It was also shown that acetan contains acetyl residues and that using [14C]acetyl CoA as donor, radioactivity was detected both at the polysaccharide and at the prenyl-linked oligosaccharide stage. |
| title |
In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| title_short |
In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| title_full |
In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| title_fullStr |
In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| title_full_unstemmed |
In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| title_sort |
in vitro biosynthesis of acetan using electroporated acetobacter xylinum cells as enzyme preparations |
| publishDate |
1993 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221287_v139_n11_p2745_Semino http://hdl.handle.net/20.500.12110/paper_00221287_v139_n11_p2745_Semino |
| _version_ |
1768545249784758272 |