Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization

The thermal stability of enzyme invertase in reduced-moisture model systems of maltodextrin (MD), polyvinylpyrrolidone (PVP; MW 40,000) and trehalose heated at 90°C was studied. Significant invertase inactivation was observed in heated glassy PVP and MD systems kept well below their glass transition...

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Detalles Bibliográficos
Autores principales: Cardona, Silvia T., Schebor, Carolina C., Buera, María del Pilar
Publicado: 1997
Materias:
Crystallization
Glass transition
Invertase
Thermal inactivation
Trehalose
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221147_v62_n1_p105_Cardona
http://hdl.handle.net/20.500.12110/paper_00221147_v62_n1_p105_Cardona
Aporte de:
Aportado por : Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires .
id paper:paper_00221147_v62_n1_p105_Cardona
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Crystallization
Glass transition
Invertase
Thermal inactivation
Trehalose
spellingShingle Crystallization
Glass transition
Invertase
Thermal inactivation
Trehalose
Cardona, Silvia T.
Schebor, Carolina C.
Buera, María del Pilar
Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
topic_facet Crystallization
Glass transition
Invertase
Thermal inactivation
Trehalose
description The thermal stability of enzyme invertase in reduced-moisture model systems of maltodextrin (MD), polyvinylpyrrolidone (PVP; MW 40,000) and trehalose heated at 90°C was studied. Significant invertase inactivation was observed in heated glassy PVP and MD systems kept well below their glass transition temperature (T(g)), but the enzyme was fairly stable in rubbery trehalose systems. However, at moisture contents which allowed trehalose crystallization rapid thermal inactivation of invertase was observed. Invertase inactivation in heated PVP, MD and trehalose systems of reduced-moisture could not be predicted on the basis of glass transition and this was particularly true for trehalose. Conditions which would allow collapse of the systems and crystallization of trehalose were fairly well predicted based oil the estimated T(g) of model systems.
author Cardona, Silvia T.
Schebor, Carolina C.
Buera, María del Pilar
author_facet Cardona, Silvia T.
Schebor, Carolina C.
Buera, María del Pilar
author_sort Cardona, Silvia T.
title Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
title_short Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
title_full Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
title_fullStr Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
title_full_unstemmed Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
title_sort thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and trehalose crystallization
publishDate 1997
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221147_v62_n1_p105_Cardona
http://hdl.handle.net/20.500.12110/paper_00221147_v62_n1_p105_Cardona
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