Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation

Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enz...

Descripción completa

Detalles Bibliográficos
Autores principales:	Soto, Gabriela Cynthia, Stritzler, Margarita, Ardila, Fernando Jorge, Ayub, Nicolás Daniel
Publicado:	2011
Materias:	Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v62_n15_p5699_Soto http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00220957_v62_n15_p5699_Soto
record_format	dspace
spelling	paper:paper_00220957_v62_n15_p5699_Soto2023-06-08T14:45:43Z Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation Soto, Gabriela Cynthia Stritzler, Margarita Ardila, Fernando Jorge Ayub, Nicolás Daniel Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enzymatic activity was experimentally demonstrated in planta and in heterologous systems. The condensation reaction by MsAACT1 was proved to be inhibited by CoA suggesting a negative feedback regulation of isoprenoid production. Real-time RT-PCR analysis indicated that MsAACT1 expression is highly increased in roots and leaves under cold and salinity stress. Treatment with mevastatin, a specific inhibitor of the MVA pathway, resulted in a decrease in squalene production, antioxidant activity, and the survival of stressed plants. As expected, the presence of mevastatin did not change chlorophyll and carotenoid levels, isoprenoids synthesized via the plastidial MVA-independent pathway. The addition of vitamin C suppressed the sensitive phenotype of plants challenged with mevastatin, suggesting a critical function of the MVA pathway in abiotic stress-inducible antioxidant defence. MsAACT1 over-expressing transgenic plants showed salinity tolerance comparable with empty vector transformed plants and enhanced production of squalene without altering the 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) activity in salt-stress conditions. Thus, acetoacetyl-CoA thiolase is a regulatory enzyme in isoprenoid biosynthesis involved in abiotic stress adaptation. © 2011 The Author. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stritzler, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ardila, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v62_n15_p5699_Soto http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene
spellingShingle	Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene Soto, Gabriela Cynthia Stritzler, Margarita Ardila, Fernando Jorge Ayub, Nicolás Daniel Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
topic_facet	Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene
description	Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enzymatic activity was experimentally demonstrated in planta and in heterologous systems. The condensation reaction by MsAACT1 was proved to be inhibited by CoA suggesting a negative feedback regulation of isoprenoid production. Real-time RT-PCR analysis indicated that MsAACT1 expression is highly increased in roots and leaves under cold and salinity stress. Treatment with mevastatin, a specific inhibitor of the MVA pathway, resulted in a decrease in squalene production, antioxidant activity, and the survival of stressed plants. As expected, the presence of mevastatin did not change chlorophyll and carotenoid levels, isoprenoids synthesized via the plastidial MVA-independent pathway. The addition of vitamin C suppressed the sensitive phenotype of plants challenged with mevastatin, suggesting a critical function of the MVA pathway in abiotic stress-inducible antioxidant defence. MsAACT1 over-expressing transgenic plants showed salinity tolerance comparable with empty vector transformed plants and enhanced production of squalene without altering the 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) activity in salt-stress conditions. Thus, acetoacetyl-CoA thiolase is a regulatory enzyme in isoprenoid biosynthesis involved in abiotic stress adaptation. © 2011 The Author.
author	Soto, Gabriela Cynthia Stritzler, Margarita Ardila, Fernando Jorge Ayub, Nicolás Daniel
author_facet	Soto, Gabriela Cynthia Stritzler, Margarita Ardila, Fernando Jorge Ayub, Nicolás Daniel
author_sort	Soto, Gabriela Cynthia
title	Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
title_short	Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
title_full	Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
title_fullStr	Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
title_full_unstemmed	Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
title_sort	acetoacetyl-coa thiolase regulates the mevalonate pathway during abiotic stress adaptation
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v62_n15_p5699_Soto http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto
work_keys_str_mv	AT sotogabrielacynthia acetoacetylcoathiolaseregulatesthemevalonatepathwayduringabioticstressadaptation AT stritzlermargarita acetoacetylcoathiolaseregulatesthemevalonatepathwayduringabioticstressadaptation AT ardilafernandojorge acetoacetylcoathiolaseregulatesthemevalonatepathwayduringabioticstressadaptation AT ayubnicolasdaniel acetoacetylcoathiolaseregulatesthemevalonatepathwayduringabioticstressadaptation
_version_	1768546706657378304

Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation

Ejemplares similares