Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress

The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activit...

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Autores principales:	Rodríguez, Andrés Alberto, Menéndez, Ana Bernardina
Publicado:	2009
Materias:	Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v60_n15_p4249_Rodriguez http://hdl.handle.net/20.500.12110/paper_00220957_v60_n15_p4249_Rodriguez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00220957_v60_n15_p4249_Rodriguez
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spelling	paper:paper_00220957_v60_n15_p4249_Rodriguez2023-06-08T14:45:42Z Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress Rodríguez, Andrés Alberto Menéndez, Ana Bernardina Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxidase was up to 20-fold higher than that of the copper-containing amine oxidase. Measurements of H2O2, ·O2-, and HO· production in the presence or absence of the polyamine oxidase inhibitors 1,19-bis- (ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane suggest that, in salinized plants, the oxidation of free apoplastic polyamines by polyamine oxidase by would be the main source of reactive oxygen species in the elongation zone of maize leaf blades. This effect is probably due to increased substrate availability. Incubation with 200 μM spermine doubled segment elongation, whereas the addition of 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane to 200 μM spermine attenuated and reversed the last effect, respectively. Similarly, the addition of MnCl2 (an ·O2- dismutating agent) or the HO· scavenger sodium benzoate along with spermine, annulled the elongating effect of the polyamine on the salinized segments. As a whole, the results obtained here demonstrated that, under salinity, polyamine oxidase activity provides a significant production of reactive oxygen species in the apoplast which contributes to 25-30% of the maize leaf blade elongation. Fil:Rodríguez, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v60_n15_p4249_Rodriguez http://hdl.handle.net/20.500.12110/paper_00220957_v60_n15_p4249_Rodriguez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays
spellingShingle	Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays Rodríguez, Andrés Alberto Menéndez, Ana Bernardina Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
topic_facet	Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays
description	The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxidase was up to 20-fold higher than that of the copper-containing amine oxidase. Measurements of H2O2, ·O2-, and HO· production in the presence or absence of the polyamine oxidase inhibitors 1,19-bis- (ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane suggest that, in salinized plants, the oxidation of free apoplastic polyamines by polyamine oxidase by would be the main source of reactive oxygen species in the elongation zone of maize leaf blades. This effect is probably due to increased substrate availability. Incubation with 200 μM spermine doubled segment elongation, whereas the addition of 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane to 200 μM spermine attenuated and reversed the last effect, respectively. Similarly, the addition of MnCl2 (an ·O2- dismutating agent) or the HO· scavenger sodium benzoate along with spermine, annulled the elongating effect of the polyamine on the salinized segments. As a whole, the results obtained here demonstrated that, under salinity, polyamine oxidase activity provides a significant production of reactive oxygen species in the apoplast which contributes to 25-30% of the maize leaf blade elongation.
author	Rodríguez, Andrés Alberto Menéndez, Ana Bernardina
author_facet	Rodríguez, Andrés Alberto Menéndez, Ana Bernardina
author_sort	Rodríguez, Andrés Alberto
title	Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
title_short	Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
title_full	Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
title_fullStr	Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
title_full_unstemmed	Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
title_sort	polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v60_n15_p4249_Rodriguez http://hdl.handle.net/20.500.12110/paper_00220957_v60_n15_p4249_Rodriguez
work_keys_str_mv	AT rodriguezandresalberto polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress AT menendezanabernardina polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress
_version_	1768544763535949824

Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress

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