UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers

Envelope membranes were isolated from potato tuber amyloplast by a discontinuous sucrose density gradient and high speed centrifugation. These membranes catalyzed the transfer of [14C]glucose from UDP-[14C]glucose to endogenous sterol acceptors and, in turn, catalyzed the esterification of steryl gl...

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Autores principales: Catz, Diana Silvia, Tandecarz, Juana Sara
Publicado: 1985
Materias:
Amyloplast membrane
Solanum tuberosum
Sterol glucosylation
Steryl glucoside acylation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v36_n4_p602_Catz
http://hdl.handle.net/20.500.12110/paper_00220957_v36_n4_p602_Catz
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00220957_v36_n4_p602_Catz
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spelling paper:paper_00220957_v36_n4_p602_Catz2023-06-08T14:45:41Z UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers Catz, Diana Silvia Tandecarz, Juana Sara Amyloplast membrane Solanum tuberosum Sterol glucosylation Steryl glucoside acylation Envelope membranes were isolated from potato tuber amyloplast by a discontinuous sucrose density gradient and high speed centrifugation. These membranes catalyzed the transfer of [14C]glucose from UDP-[14C]glucose to endogenous sterol acceptors and, in turn, catalyzed the esterification of steryl glucosides with fatty acids from an endogenous acyl donor. The synthesis of steryl glucosides was stimulated in the presence of Triton. X-100, while formation of acyl steryl glucosides was inhibited by the detergent. However, in the presence of an added sterol acceptor and Triton X-100, the inhibition of acyl steryl glucoside synthesis was overcome by the addition of phosphatidylethanolamine. The enzyme involved in steryl glucoside formation was solubilized by treatment of the envelope membranes with 0.3% Triton X-100. The solubilized enzyme had an almost absolute requirement for sterol acceptors. © 1985 Oxford University Press. Fil:Catz, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v36_n4_p602_Catz http://hdl.handle.net/20.500.12110/paper_00220957_v36_n4_p602_Catz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Amyloplast membrane
Solanum tuberosum
Sterol glucosylation
Steryl glucoside acylation
spellingShingle Amyloplast membrane
Solanum tuberosum
Sterol glucosylation
Steryl glucoside acylation
Catz, Diana Silvia
Tandecarz, Juana Sara
UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
topic_facet Amyloplast membrane
Solanum tuberosum
Sterol glucosylation
Steryl glucoside acylation
description Envelope membranes were isolated from potato tuber amyloplast by a discontinuous sucrose density gradient and high speed centrifugation. These membranes catalyzed the transfer of [14C]glucose from UDP-[14C]glucose to endogenous sterol acceptors and, in turn, catalyzed the esterification of steryl glucosides with fatty acids from an endogenous acyl donor. The synthesis of steryl glucosides was stimulated in the presence of Triton. X-100, while formation of acyl steryl glucosides was inhibited by the detergent. However, in the presence of an added sterol acceptor and Triton X-100, the inhibition of acyl steryl glucoside synthesis was overcome by the addition of phosphatidylethanolamine. The enzyme involved in steryl glucoside formation was solubilized by treatment of the envelope membranes with 0.3% Triton X-100. The solubilized enzyme had an almost absolute requirement for sterol acceptors. © 1985 Oxford University Press.
author Catz, Diana Silvia
Tandecarz, Juana Sara
author_facet Catz, Diana Silvia
Tandecarz, Juana Sara
author_sort Catz, Diana Silvia
title UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
title_short UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
title_full UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
title_fullStr UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
title_full_unstemmed UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
title_sort udp-glucose: sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers
publishDate 1985
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v36_n4_p602_Catz
http://hdl.handle.net/20.500.12110/paper_00220957_v36_n4_p602_Catz
work_keys_str_mv AT catzdianasilvia udpglucosesterolglucosyltransferaseandasterylglucosideacyltransferaseactivityinamyloplastmembranesfrompotatotubers
AT tandecarzjuanasara udpglucosesterolglucosyltransferaseandasterylglucosideacyltransferaseactivityinamyloplastmembranesfrompotatotubers
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