Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature
Haematophagous insects suffer big changes in water needs under different levels of starvation. Rhodnius prolixus is the most important haematophagous vector of Chagas disease in the north of South America and a model organism in insect physiology. Although there have been some studies on patterns of...
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paper:paper_00220949_v217_n24_p4414_Rolandi2023-06-08T14:45:38Z Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature Schilman, Pablo Ernesto CO2 emission rate Cuticular permeability Flow-through respirometry Respiratory water loss Hexapoda Rhodnius prolixus carbon dioxide water animal energy metabolism metabolism permeability physiological stress physiology Rhodnius starvation temperature thermoregulation Animals Carbon Dioxide Energy Metabolism Permeability Rhodnius Starvation Stress, Physiological Temperature Water Water Loss, Insensible Haematophagous insects suffer big changes in water needs under different levels of starvation. Rhodnius prolixus is the most important haematophagous vector of Chagas disease in the north of South America and a model organism in insect physiology. Although there have been some studies on patterns of gas exchange and metabolic rates, there is little information regarding water loss in R. prolixus. We investigated whether there is any modulation of water loss and metabolic rate under different requirements for saving water. We measured simultaneously CO2 production, water emission and activity in individual insects in real time by open-flow respirometry at different temperatures (15, 25 and 35°C) and post-feeding days (0, 5, 13 and 29). We found: (1) a clear drop in metabolic rate between 5 and 13 days after feeding that cannot be explained by activity and (2) a decrease in water loss rate with increasing starvation level, by a decrease in cuticular water loss during the first 5 days after feeding and a drop in the respiratory component thereafter. We calculated the surface area of the insects and estimated cuticular permeability. In addition, we analysed the pattern of gas exchange; the change from a cyclic to a continuous pattern was affected by temperature and activity, but it was not affected by the level of starvation. Modulation of metabolic and water loss rates with temperature and starvation could help R. prolixus to be more flexible in tolerating different periods of starvation, which is adaptive in a changing environment with the uncertainty of finding a suitable host. © 2014. Published by The Company of Biologists Ltd. Fil:Schilman, P.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v217_n24_p4414_Rolandi http://hdl.handle.net/20.500.12110/paper_00220949_v217_n24_p4414_Rolandi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CO2 emission rate Cuticular permeability Flow-through respirometry Respiratory water loss Hexapoda Rhodnius prolixus carbon dioxide water animal energy metabolism metabolism permeability physiological stress physiology Rhodnius starvation temperature thermoregulation Animals Carbon Dioxide Energy Metabolism Permeability Rhodnius Starvation Stress, Physiological Temperature Water Water Loss, Insensible |
| spellingShingle |
CO2 emission rate Cuticular permeability Flow-through respirometry Respiratory water loss Hexapoda Rhodnius prolixus carbon dioxide water animal energy metabolism metabolism permeability physiological stress physiology Rhodnius starvation temperature thermoregulation Animals Carbon Dioxide Energy Metabolism Permeability Rhodnius Starvation Stress, Physiological Temperature Water Water Loss, Insensible Schilman, Pablo Ernesto Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| topic_facet |
CO2 emission rate Cuticular permeability Flow-through respirometry Respiratory water loss Hexapoda Rhodnius prolixus carbon dioxide water animal energy metabolism metabolism permeability physiological stress physiology Rhodnius starvation temperature thermoregulation Animals Carbon Dioxide Energy Metabolism Permeability Rhodnius Starvation Stress, Physiological Temperature Water Water Loss, Insensible |
| description |
Haematophagous insects suffer big changes in water needs under different levels of starvation. Rhodnius prolixus is the most important haematophagous vector of Chagas disease in the north of South America and a model organism in insect physiology. Although there have been some studies on patterns of gas exchange and metabolic rates, there is little information regarding water loss in R. prolixus. We investigated whether there is any modulation of water loss and metabolic rate under different requirements for saving water. We measured simultaneously CO2 production, water emission and activity in individual insects in real time by open-flow respirometry at different temperatures (15, 25 and 35°C) and post-feeding days (0, 5, 13 and 29). We found: (1) a clear drop in metabolic rate between 5 and 13 days after feeding that cannot be explained by activity and (2) a decrease in water loss rate with increasing starvation level, by a decrease in cuticular water loss during the first 5 days after feeding and a drop in the respiratory component thereafter. We calculated the surface area of the insects and estimated cuticular permeability. In addition, we analysed the pattern of gas exchange; the change from a cyclic to a continuous pattern was affected by temperature and activity, but it was not affected by the level of starvation. Modulation of metabolic and water loss rates with temperature and starvation could help R. prolixus to be more flexible in tolerating different periods of starvation, which is adaptive in a changing environment with the uncertainty of finding a suitable host. © 2014. Published by The Company of Biologists Ltd. |
| author |
Schilman, Pablo Ernesto |
| author_facet |
Schilman, Pablo Ernesto |
| author_sort |
Schilman, Pablo Ernesto |
| title |
Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| title_short |
Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| title_full |
Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| title_fullStr |
Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| title_full_unstemmed |
Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature |
| title_sort |
metabolism and water loss rate of the haematophagous insect rhodnius prolixus: effect of starvation and temperature |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v217_n24_p4414_Rolandi http://hdl.handle.net/20.500.12110/paper_00220949_v217_n24_p4414_Rolandi |
| work_keys_str_mv |
AT schilmanpabloernesto metabolismandwaterlossrateofthehaematophagousinsectrhodniusprolixuseffectofstarvationandtemperature |
| _version_ |
1768544306210013184 |