Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity

We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and b...

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Autores principales:	Luquet, Carlos Marcelo, Halperin, Julia, Marqués, Roberto Oscar
Publicado:	2002
Materias:	Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00220949_v205_n1_p71_Luquet
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spelling	paper:paper_00220949_v205_n1_p71_Luquet2023-06-08T14:45:31Z Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity Luquet, Carlos Marcelo Halperin, Julia Marqués, Roberto Oscar Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10%‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, -10.2±1.6, -10.8±1.3 and -6.7±1.3mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30%‰ to 20%‰ or 10%‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2 (5mmoll-1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 μequiv g-1h-1. Gill 6 of crabs acclimated to high salinity produced TEPDs of -1.5±0.1 and -1.3±0.09mV after perfusion with 30%‰ or 40%‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 μequiv g-1h-1 was recorded in gill 6 perfused with 38%‰ saline. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Halperin, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marques, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus
spellingShingle	Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus Luquet, Carlos Marcelo Halperin, Julia Marqués, Roberto Oscar Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
topic_facet	Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus
description	We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10%‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, -10.2±1.6, -10.8±1.3 and -6.7±1.3mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30%‰ to 20%‰ or 10%‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2 (5mmoll-1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 μequiv g-1h-1. Gill 6 of crabs acclimated to high salinity produced TEPDs of -1.5±0.1 and -1.3±0.09mV after perfusion with 30%‰ or 40%‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 μequiv g-1h-1 was recorded in gill 6 perfused with 38%‰ saline.
author	Luquet, Carlos Marcelo Halperin, Julia Marqués, Roberto Oscar
author_facet	Luquet, Carlos Marcelo Halperin, Julia Marqués, Roberto Oscar
author_sort	Luquet, Carlos Marcelo
title	Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
title_short	Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
title_full	Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
title_fullStr	Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
title_full_unstemmed	Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
title_sort	transepithelial potential differences and na+ flux in isolated perfused gills of the crab chasmagnathus granulatus (grapsidae) acclimated to hyper- and hypo-salinity
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet
work_keys_str_mv	AT luquetcarlosmarcelo transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT halperinjulia transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT marquesrobertooscar transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity
_version_	1768544853818343424

Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity

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