Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

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We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development havebeen studied, but the results are still controversial.Herein,weshowthemodulatory action of thyroid status on the in vivo growth...

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Autor principal:	Barreiro Arcos, María Laura
Publicado:	2014
Materias:	Angiogenesis Cell cycle T lymphoma Thyroid hormones beta 2 microglobulin caspase 3 cyclin A2 cyclin B1 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase inhibitor 1 cyclin dependent kinase inhibitor 1B cyclin dependent kinase inhibitor 2A cyclin dependent kinase inhibitor 2B cyclin E liothyronine phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein p53 retinoblastoma protein thyroid hormone thyrotropin thyroxine angiogenesis animal experiment animal tissue article cancer growth cancer size cancer survival cell division controlled study female hyperthyroidism hypothyroidism immunoblotting in vivo study liothyronine blood level mouse nonhuman priority journal protein expression T cell lymphoma thyroxine blood level cell cycle thyroid hormones Animals Apoptosis Caspase 3 Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Cyclin D1 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Female Hyperthyroidism Hypothyroidism Lymphoma, T-Cell Mice Mice, Inbred C57BL Neoplasm Transplantation Neovascularization, Pathologic Proliferating Cell Nuclear Antigen Thyroid Gland Tumor Suppressor Protein p53
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v222_n2_p243_Sterle http://hdl.handle.net/20.500.12110/paper_00220795_v222_n2_p243_Sterle
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00220795_v222_n2_p243_Sterle2023-06-08T14:45:30Z Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis Barreiro Arcos, María Laura Angiogenesis Cell cycle T lymphoma Thyroid hormones beta 2 microglobulin caspase 3 cyclin A2 cyclin B1 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase inhibitor 1 cyclin dependent kinase inhibitor 1B cyclin dependent kinase inhibitor 2A cyclin dependent kinase inhibitor 2B cyclin E liothyronine phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein p53 retinoblastoma protein thyroid hormone thyrotropin thyroxine angiogenesis animal experiment animal tissue article cancer growth cancer size cancer survival cell division controlled study female hyperthyroidism hypothyroidism immunoblotting in vivo study liothyronine blood level mouse nonhuman priority journal protein expression T cell lymphoma thyroxine blood level angiogenesis cell cycle T lymphoma thyroid hormones Animals Apoptosis Caspase 3 Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Cyclin D1 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Female Hyperthyroidism Hypothyroidism Lymphoma, T-Cell Mice Mice, Inbred C57BL Neoplasm Transplantation Neovascularization, Pathologic Proliferating Cell Nuclear Antigen Thyroid Gland Tumor Suppressor Protein p53 We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development havebeen studied, but the results are still controversial.Herein,weshowthemodulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistentwiththe rate of cell division determined by stainingtumor cellswith carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly fromthe euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increasedinhypothyroidmice. Intratumoral andperitumoral vasculogenesiswas increasedonly in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis. © 2014 Society for Endocrinology. Fil:Barreiro Arcos, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v222_n2_p243_Sterle http://hdl.handle.net/20.500.12110/paper_00220795_v222_n2_p243_Sterle
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Angiogenesis Cell cycle T lymphoma Thyroid hormones beta 2 microglobulin caspase 3 cyclin A2 cyclin B1 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase inhibitor 1 cyclin dependent kinase inhibitor 1B cyclin dependent kinase inhibitor 2A cyclin dependent kinase inhibitor 2B cyclin E liothyronine phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein p53 retinoblastoma protein thyroid hormone thyrotropin thyroxine angiogenesis animal experiment animal tissue article cancer growth cancer size cancer survival cell division controlled study female hyperthyroidism hypothyroidism immunoblotting in vivo study liothyronine blood level mouse nonhuman priority journal protein expression T cell lymphoma thyroxine blood level angiogenesis cell cycle T lymphoma thyroid hormones Animals Apoptosis Caspase 3 Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Cyclin D1 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Female Hyperthyroidism Hypothyroidism Lymphoma, T-Cell Mice Mice, Inbred C57BL Neoplasm Transplantation Neovascularization, Pathologic Proliferating Cell Nuclear Antigen Thyroid Gland Tumor Suppressor Protein p53
spellingShingle	Angiogenesis Cell cycle T lymphoma Thyroid hormones beta 2 microglobulin caspase 3 cyclin A2 cyclin B1 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase inhibitor 1 cyclin dependent kinase inhibitor 1B cyclin dependent kinase inhibitor 2A cyclin dependent kinase inhibitor 2B cyclin E liothyronine phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein p53 retinoblastoma protein thyroid hormone thyrotropin thyroxine angiogenesis animal experiment animal tissue article cancer growth cancer size cancer survival cell division controlled study female hyperthyroidism hypothyroidism immunoblotting in vivo study liothyronine blood level mouse nonhuman priority journal protein expression T cell lymphoma thyroxine blood level angiogenesis cell cycle T lymphoma thyroid hormones Animals Apoptosis Caspase 3 Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Cyclin D1 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Female Hyperthyroidism Hypothyroidism Lymphoma, T-Cell Mice Mice, Inbred C57BL Neoplasm Transplantation Neovascularization, Pathologic Proliferating Cell Nuclear Antigen Thyroid Gland Tumor Suppressor Protein p53 Barreiro Arcos, María Laura Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
topic_facet	Angiogenesis Cell cycle T lymphoma Thyroid hormones beta 2 microglobulin caspase 3 cyclin A2 cyclin B1 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase inhibitor 1 cyclin dependent kinase inhibitor 1B cyclin dependent kinase inhibitor 2A cyclin dependent kinase inhibitor 2B cyclin E liothyronine phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein p53 retinoblastoma protein thyroid hormone thyrotropin thyroxine angiogenesis animal experiment animal tissue article cancer growth cancer size cancer survival cell division controlled study female hyperthyroidism hypothyroidism immunoblotting in vivo study liothyronine blood level mouse nonhuman priority journal protein expression T cell lymphoma thyroxine blood level angiogenesis cell cycle T lymphoma thyroid hormones Animals Apoptosis Caspase 3 Cell Cycle Proteins Cell Line, Tumor Cell Proliferation Cyclin D1 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Female Hyperthyroidism Hypothyroidism Lymphoma, T-Cell Mice Mice, Inbred C57BL Neoplasm Transplantation Neovascularization, Pathologic Proliferating Cell Nuclear Antigen Thyroid Gland Tumor Suppressor Protein p53
description	We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development havebeen studied, but the results are still controversial.Herein,weshowthemodulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistentwiththe rate of cell division determined by stainingtumor cellswith carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly fromthe euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increasedinhypothyroidmice. Intratumoral andperitumoral vasculogenesiswas increasedonly in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis. © 2014 Society for Endocrinology.
author	Barreiro Arcos, María Laura
author_facet	Barreiro Arcos, María Laura
author_sort	Barreiro Arcos, María Laura
title	Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_short	Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full	Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_fullStr	Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full_unstemmed	Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_sort	thyroid status modulates t lymphoma growth via cell cycle regulatory proteins and angiogenesis
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v222_n2_p243_Sterle http://hdl.handle.net/20.500.12110/paper_00220795_v222_n2_p243_Sterle
work_keys_str_mv	AT barreiroarcosmarialaura thyroidstatusmodulatestlymphomagrowthviacellcycleregulatoryproteinsandangiogenesis
_version_	1768546706469683200

Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

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