GH administration patterns differently regulate epidermal growth factor signaling
GH administration protocols imply frequent s.c. injections, resulting in suboptimal compliance. Therefore, there is interest in developing delivery systems for sustained release of the hormone. However, GH has different actions depending on its continuous or pulsatile plasma concentration pattern. G...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n2_p309_Diaz http://hdl.handle.net/20.500.12110/paper_00220795_v221_n2_p309_Diaz |
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paper:paper_00220795_v221_n2_p309_Diaz2023-06-08T14:45:29Z GH administration patterns differently regulate epidermal growth factor signaling Epidermal growth factor Growth hormone Intracellular signaling Liver epidermal growth factor growth hormone EGFR protein, mouse epidermal growth factor epidermal growth factor receptor growth hormone adult animal cell animal experiment animal model article cell activation cell proliferation controlled study drug blood level drug safety female gene expression liver male mouse nonhuman osmotic pump priority journal protein content protein expression protein phosphorylation signal transduction upregulation animal blood cell cycle drug administration drug effect gene expression regulation genetics infusion pump injection intracellular signaling metabolism signal transduction epidermal growth factor growth hormone intracellular signaling liver Animals Cell Cycle Drug Administration Schedule Epidermal Growth Factor Female Gene Expression Regulation Growth Hormone Infusion Pumps Injections Male Mice Receptor, Epidermal Growth Factor Signal Transduction GH administration protocols imply frequent s.c. injections, resulting in suboptimal compliance. Therefore, there is interest in developing delivery systems for sustained release of the hormone. However, GH has different actions depending on its continuous or pulsatile plasma concentration pattern. GH levels and circulating concentration patterns could be involved in the regulation of epidermal growth factor receptor (EGFR) expression in liver. Aberrant expression of this receptor and/or its hyperactivation has been associated with the pathogenesis of different types of carcinoma. Considering that one of the adverse effects associated with GH overexpression and chronic use of GH is the increased incidence of malignancies, the aim of this study was to analyze the effects of GH plasma concentration patterns on EGFR expression and signaling in livers of mice. For this purpose, GH was administered by s.c. daily injections to produce an intermittent plasma pattern or by osmotic pumps to provoke a continuously elevated GH concentration. Intermittent injections of GH induced upregulation of liver EGFR content, augmented the response to EGF, and the induction of proteins involved in promotion of cell proliferation in female mice. In contrast, continuous GH delivery in male mice was associated with diminished EGFR in liver and decreased EGF-induced signaling and expression of early genes. The results indicate that sustained delivery systems that allow continuous GH plasma patterns would be beneficial in terms of treatment safety with regard to the actions of GH on EGFR signaling and its promitogenic activity. © 2014 Society for Endocrinology. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n2_p309_Diaz http://hdl.handle.net/20.500.12110/paper_00220795_v221_n2_p309_Diaz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epidermal growth factor Growth hormone Intracellular signaling Liver epidermal growth factor growth hormone EGFR protein, mouse epidermal growth factor epidermal growth factor receptor growth hormone adult animal cell animal experiment animal model article cell activation cell proliferation controlled study drug blood level drug safety female gene expression liver male mouse nonhuman osmotic pump priority journal protein content protein expression protein phosphorylation signal transduction upregulation animal blood cell cycle drug administration drug effect gene expression regulation genetics infusion pump injection intracellular signaling metabolism signal transduction epidermal growth factor growth hormone intracellular signaling liver Animals Cell Cycle Drug Administration Schedule Epidermal Growth Factor Female Gene Expression Regulation Growth Hormone Infusion Pumps Injections Male Mice Receptor, Epidermal Growth Factor Signal Transduction |
| spellingShingle |
Epidermal growth factor Growth hormone Intracellular signaling Liver epidermal growth factor growth hormone EGFR protein, mouse epidermal growth factor epidermal growth factor receptor growth hormone adult animal cell animal experiment animal model article cell activation cell proliferation controlled study drug blood level drug safety female gene expression liver male mouse nonhuman osmotic pump priority journal protein content protein expression protein phosphorylation signal transduction upregulation animal blood cell cycle drug administration drug effect gene expression regulation genetics infusion pump injection intracellular signaling metabolism signal transduction epidermal growth factor growth hormone intracellular signaling liver Animals Cell Cycle Drug Administration Schedule Epidermal Growth Factor Female Gene Expression Regulation Growth Hormone Infusion Pumps Injections Male Mice Receptor, Epidermal Growth Factor Signal Transduction GH administration patterns differently regulate epidermal growth factor signaling |
| topic_facet |
Epidermal growth factor Growth hormone Intracellular signaling Liver epidermal growth factor growth hormone EGFR protein, mouse epidermal growth factor epidermal growth factor receptor growth hormone adult animal cell animal experiment animal model article cell activation cell proliferation controlled study drug blood level drug safety female gene expression liver male mouse nonhuman osmotic pump priority journal protein content protein expression protein phosphorylation signal transduction upregulation animal blood cell cycle drug administration drug effect gene expression regulation genetics infusion pump injection intracellular signaling metabolism signal transduction epidermal growth factor growth hormone intracellular signaling liver Animals Cell Cycle Drug Administration Schedule Epidermal Growth Factor Female Gene Expression Regulation Growth Hormone Infusion Pumps Injections Male Mice Receptor, Epidermal Growth Factor Signal Transduction |
| description |
GH administration protocols imply frequent s.c. injections, resulting in suboptimal compliance. Therefore, there is interest in developing delivery systems for sustained release of the hormone. However, GH has different actions depending on its continuous or pulsatile plasma concentration pattern. GH levels and circulating concentration patterns could be involved in the regulation of epidermal growth factor receptor (EGFR) expression in liver. Aberrant expression of this receptor and/or its hyperactivation has been associated with the pathogenesis of different types of carcinoma. Considering that one of the adverse effects associated with GH overexpression and chronic use of GH is the increased incidence of malignancies, the aim of this study was to analyze the effects of GH plasma concentration patterns on EGFR expression and signaling in livers of mice. For this purpose, GH was administered by s.c. daily injections to produce an intermittent plasma pattern or by osmotic pumps to provoke a continuously elevated GH concentration. Intermittent injections of GH induced upregulation of liver EGFR content, augmented the response to EGF, and the induction of proteins involved in promotion of cell proliferation in female mice. In contrast, continuous GH delivery in male mice was associated with diminished EGFR in liver and decreased EGF-induced signaling and expression of early genes. The results indicate that sustained delivery systems that allow continuous GH plasma patterns would be beneficial in terms of treatment safety with regard to the actions of GH on EGFR signaling and its promitogenic activity. © 2014 Society for Endocrinology. |
| title |
GH administration patterns differently regulate epidermal growth factor signaling |
| title_short |
GH administration patterns differently regulate epidermal growth factor signaling |
| title_full |
GH administration patterns differently regulate epidermal growth factor signaling |
| title_fullStr |
GH administration patterns differently regulate epidermal growth factor signaling |
| title_full_unstemmed |
GH administration patterns differently regulate epidermal growth factor signaling |
| title_sort |
gh administration patterns differently regulate epidermal growth factor signaling |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n2_p309_Diaz http://hdl.handle.net/20.500.12110/paper_00220795_v221_n2_p309_Diaz |
| _version_ |
1768542631057424384 |