VIP contribution to the decidualization program: Regulatory T cell recruitment
During early pregnancy, the human uterus undergoes profound tissue remodeling characterized by leukocyte invasion and production of proinflammatory cytokines, followed by tissue repair and tolerance maintenance induction. Vasoactive intestinal peptide (VIP) is produced by trophoblast cells and modul...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n1_p121_Grasso http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p121_Grasso |
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paper:paper_00220795_v221_n1_p121_Grasso2023-06-08T14:45:29Z VIP contribution to the decidualization program: Regulatory T cell recruitment Decidualization Early pregnancy Human endometrial stromal cells VIP lipopolysaccharide neutralizing antibody progesterone RANTES vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor 1 article CD25+ T lymphocyte CD4+ T lymphocyte controlled study decidualization endometrium cell female female fertility first trimester pregnancy flow cytometry fluorescence foxp3 t lymphocyte hormone action hormone synthesis human human cell human endometrial stromal cell line implantation in vitro study inflammation lymphocyte differentiation lymphocyte function lymphocyte migration macrophage neutrophil peripheral blood mononuclear cell priority journal protein expression regulatory t cell recruitment regulatory T lymphocyte stroma cell T lymphocyte decidualization early pregnancy human endometrial stromal cells VIP Cell Differentiation Chemokine CCL5 Decidua Embryo Implantation Female Humans Pregnancy Progesterone Stromal Cells T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide During early pregnancy, the human uterus undergoes profound tissue remodeling characterized by leukocyte invasion and production of proinflammatory cytokines, followed by tissue repair and tolerance maintenance induction. Vasoactive intestinal peptide (VIP) is produced by trophoblast cells and modulates the maternal immune response toward a tolerogenic profile. Here, we evaluated the contribution of the VIP/VPAC to endometrial renewal, inducing decidualization and the recruitment of induced regulatory T cells (iTregs) that accompany the implantation period. For that purpose, we used an in vitro model of decidualization with a human endometrial stromal cell line (HESC) stimulated with progesterone (P4) and lipopolysaccharide (LPS) simulating the inflammatory response during implantation and human iTregs (CD4+CD25+FOXP3+) differentiated from naïve T cells obtained from peripheral blood mononuclear cells of fertile women. We observed that VIP and its receptor VPAC1 are constitutively expressed in HESCs and that P4 increased VIP expression. Moreover, in HESC VIP induced expression of RANTES (CCL5), one of the main chemokines involved in T cell recruitment, and this effect is enhanced by the presence of P4 and LPS. Finally, assays of the migration of iTregs toward conditioned media from HESCs revealed that endogenous VIP production induced by P4 and LPS and RANTES production were involved, as anti-RANTES neutralizing Ab or VIP antagonist prevented their migration.We conclude that VIPmay have an active role in the decidualization process, thus contributing to recruitment of iTregs toward endometrial stromal cells by increasing RANTES expression in a P4-dependent manner. © 2014 Society for Endocrinology Printed in Great Britain. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n1_p121_Grasso http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p121_Grasso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Decidualization Early pregnancy Human endometrial stromal cells VIP lipopolysaccharide neutralizing antibody progesterone RANTES vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor 1 article CD25+ T lymphocyte CD4+ T lymphocyte controlled study decidualization endometrium cell female female fertility first trimester pregnancy flow cytometry fluorescence foxp3 t lymphocyte hormone action hormone synthesis human human cell human endometrial stromal cell line implantation in vitro study inflammation lymphocyte differentiation lymphocyte function lymphocyte migration macrophage neutrophil peripheral blood mononuclear cell priority journal protein expression regulatory t cell recruitment regulatory T lymphocyte stroma cell T lymphocyte decidualization early pregnancy human endometrial stromal cells VIP Cell Differentiation Chemokine CCL5 Decidua Embryo Implantation Female Humans Pregnancy Progesterone Stromal Cells T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide |
| spellingShingle |
Decidualization Early pregnancy Human endometrial stromal cells VIP lipopolysaccharide neutralizing antibody progesterone RANTES vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor 1 article CD25+ T lymphocyte CD4+ T lymphocyte controlled study decidualization endometrium cell female female fertility first trimester pregnancy flow cytometry fluorescence foxp3 t lymphocyte hormone action hormone synthesis human human cell human endometrial stromal cell line implantation in vitro study inflammation lymphocyte differentiation lymphocyte function lymphocyte migration macrophage neutrophil peripheral blood mononuclear cell priority journal protein expression regulatory t cell recruitment regulatory T lymphocyte stroma cell T lymphocyte decidualization early pregnancy human endometrial stromal cells VIP Cell Differentiation Chemokine CCL5 Decidua Embryo Implantation Female Humans Pregnancy Progesterone Stromal Cells T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide VIP contribution to the decidualization program: Regulatory T cell recruitment |
| topic_facet |
Decidualization Early pregnancy Human endometrial stromal cells VIP lipopolysaccharide neutralizing antibody progesterone RANTES vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor 1 article CD25+ T lymphocyte CD4+ T lymphocyte controlled study decidualization endometrium cell female female fertility first trimester pregnancy flow cytometry fluorescence foxp3 t lymphocyte hormone action hormone synthesis human human cell human endometrial stromal cell line implantation in vitro study inflammation lymphocyte differentiation lymphocyte function lymphocyte migration macrophage neutrophil peripheral blood mononuclear cell priority journal protein expression regulatory t cell recruitment regulatory T lymphocyte stroma cell T lymphocyte decidualization early pregnancy human endometrial stromal cells VIP Cell Differentiation Chemokine CCL5 Decidua Embryo Implantation Female Humans Pregnancy Progesterone Stromal Cells T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide |
| description |
During early pregnancy, the human uterus undergoes profound tissue remodeling characterized by leukocyte invasion and production of proinflammatory cytokines, followed by tissue repair and tolerance maintenance induction. Vasoactive intestinal peptide (VIP) is produced by trophoblast cells and modulates the maternal immune response toward a tolerogenic profile. Here, we evaluated the contribution of the VIP/VPAC to endometrial renewal, inducing decidualization and the recruitment of induced regulatory T cells (iTregs) that accompany the implantation period. For that purpose, we used an in vitro model of decidualization with a human endometrial stromal cell line (HESC) stimulated with progesterone (P4) and lipopolysaccharide (LPS) simulating the inflammatory response during implantation and human iTregs (CD4+CD25+FOXP3+) differentiated from naïve T cells obtained from peripheral blood mononuclear cells of fertile women. We observed that VIP and its receptor VPAC1 are constitutively expressed in HESCs and that P4 increased VIP expression. Moreover, in HESC VIP induced expression of RANTES (CCL5), one of the main chemokines involved in T cell recruitment, and this effect is enhanced by the presence of P4 and LPS. Finally, assays of the migration of iTregs toward conditioned media from HESCs revealed that endogenous VIP production induced by P4 and LPS and RANTES production were involved, as anti-RANTES neutralizing Ab or VIP antagonist prevented their migration.We conclude that VIPmay have an active role in the decidualization process, thus contributing to recruitment of iTregs toward endometrial stromal cells by increasing RANTES expression in a P4-dependent manner. © 2014 Society for Endocrinology Printed in Great Britain. |
| title |
VIP contribution to the decidualization program: Regulatory T cell recruitment |
| title_short |
VIP contribution to the decidualization program: Regulatory T cell recruitment |
| title_full |
VIP contribution to the decidualization program: Regulatory T cell recruitment |
| title_fullStr |
VIP contribution to the decidualization program: Regulatory T cell recruitment |
| title_full_unstemmed |
VIP contribution to the decidualization program: Regulatory T cell recruitment |
| title_sort |
vip contribution to the decidualization program: regulatory t cell recruitment |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n1_p121_Grasso http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p121_Grasso |
| _version_ |
1768545220339695616 |