Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures
Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor (LIF) and interleukin (IL)-6, which belong to the cytokine family using the common gp130 signal transducer. Recently, the expression and action of two other members of this family, IL-11 and c...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v169_n3_p539_PerezCastro http://hdl.handle.net/20.500.12110/paper_00220795_v169_n3_p539_PerezCastro |
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paper:paper_00220795_v169_n3_p539_PerezCastro2023-06-08T14:45:26Z Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 growth hormone interleukin 11 interleukin 6 leukemia inhibitory factor messenger RNA prolactin receptor subunit acromegaly adenohypophysis alpha chain animal cell animal experiment article cell aggregation cell culture cell function cell line cell stimulation cell structure clinical article concentration response controlled study growth hormone release hormone release human human cell hypophysis adenoma hypophysis cell male monolayer culture nonhuman Northern blotting priority journal prolactin release prolactinoma protein expression protein family rat regulatory mechanism signal transduction Adenoma Animals Cell Aggregation Cell Culture Techniques Ciliary Neurotrophic Factor Gene Expression Regulation, Neoplastic Human Growth Hormone Humans Interleukin-11 Male Neoplasm Proteins Pituitary Gland, Anterior Pituitary Neoplasms Prolactin Rats Rats, Sprague-Dawley Receptor, Ciliary Neurotrophic Factor RNA, Messenger Tumor Cells, Cultured Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor (LIF) and interleukin (IL)-6, which belong to the cytokine family using the common gp130 signal transducer. Recently, the expression and action of two other members of this family, IL-11 and ciliary neurotrophic factor (CNTF), on different cell lines has also been demonstrated. We studied the expression of the specific receptor subunits for CNTF in mammotropic, non-functioning and somatotropic tumors and the action of CNTF and IL-11 in the regulation of hormone secretion in these and normal pituitary cells. The mRNA for the α chain specific for the CNTF receptor was detected by Northern blot in tumors secreting prolactin (PRL) and GH and in non-functioning tumors. We found that both IL-11 and CNTF exerted a similar stimulatory effect on GH mRNA expression in somatotropic monolayer cell cultures from acromegalic tumors, but these cytokines had no significant influence on GH secretion. CNTF stimulates prolactin secretion in lactotropic monolayer cell cultures from patients with prolactinoma. In monolayer cell cultures from normal rat anterior pituitary, IL-11 and CNTF had no significant effect on the release of either GH or PRL, or on GH mRNA. However, when the cells were cultured in aggregate cultures, in which the three-dimensional structure of the cells is reconstituted, both cytokines, in doses at which they had no effect on monolayer cultures, significantly stimulated both PRL and GH secretion. These data show that IL-11 and CNTF may act as regulatory factors in anterior pituitary cells, in which the three-dimensional structure of the gland is of critical importance. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v169_n3_p539_PerezCastro http://hdl.handle.net/20.500.12110/paper_00220795_v169_n3_p539_PerezCastro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 growth hormone interleukin 11 interleukin 6 leukemia inhibitory factor messenger RNA prolactin receptor subunit acromegaly adenohypophysis alpha chain animal cell animal experiment article cell aggregation cell culture cell function cell line cell stimulation cell structure clinical article concentration response controlled study growth hormone release hormone release human human cell hypophysis adenoma hypophysis cell male monolayer culture nonhuman Northern blotting priority journal prolactin release prolactinoma protein expression protein family rat regulatory mechanism signal transduction Adenoma Animals Cell Aggregation Cell Culture Techniques Ciliary Neurotrophic Factor Gene Expression Regulation, Neoplastic Human Growth Hormone Humans Interleukin-11 Male Neoplasm Proteins Pituitary Gland, Anterior Pituitary Neoplasms Prolactin Rats Rats, Sprague-Dawley Receptor, Ciliary Neurotrophic Factor RNA, Messenger Tumor Cells, Cultured |
| spellingShingle |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 growth hormone interleukin 11 interleukin 6 leukemia inhibitory factor messenger RNA prolactin receptor subunit acromegaly adenohypophysis alpha chain animal cell animal experiment article cell aggregation cell culture cell function cell line cell stimulation cell structure clinical article concentration response controlled study growth hormone release hormone release human human cell hypophysis adenoma hypophysis cell male monolayer culture nonhuman Northern blotting priority journal prolactin release prolactinoma protein expression protein family rat regulatory mechanism signal transduction Adenoma Animals Cell Aggregation Cell Culture Techniques Ciliary Neurotrophic Factor Gene Expression Regulation, Neoplastic Human Growth Hormone Humans Interleukin-11 Male Neoplasm Proteins Pituitary Gland, Anterior Pituitary Neoplasms Prolactin Rats Rats, Sprague-Dawley Receptor, Ciliary Neurotrophic Factor RNA, Messenger Tumor Cells, Cultured Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| topic_facet |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 growth hormone interleukin 11 interleukin 6 leukemia inhibitory factor messenger RNA prolactin receptor subunit acromegaly adenohypophysis alpha chain animal cell animal experiment article cell aggregation cell culture cell function cell line cell stimulation cell structure clinical article concentration response controlled study growth hormone release hormone release human human cell hypophysis adenoma hypophysis cell male monolayer culture nonhuman Northern blotting priority journal prolactin release prolactinoma protein expression protein family rat regulatory mechanism signal transduction Adenoma Animals Cell Aggregation Cell Culture Techniques Ciliary Neurotrophic Factor Gene Expression Regulation, Neoplastic Human Growth Hormone Humans Interleukin-11 Male Neoplasm Proteins Pituitary Gland, Anterior Pituitary Neoplasms Prolactin Rats Rats, Sprague-Dawley Receptor, Ciliary Neurotrophic Factor RNA, Messenger Tumor Cells, Cultured |
| description |
Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor (LIF) and interleukin (IL)-6, which belong to the cytokine family using the common gp130 signal transducer. Recently, the expression and action of two other members of this family, IL-11 and ciliary neurotrophic factor (CNTF), on different cell lines has also been demonstrated. We studied the expression of the specific receptor subunits for CNTF in mammotropic, non-functioning and somatotropic tumors and the action of CNTF and IL-11 in the regulation of hormone secretion in these and normal pituitary cells. The mRNA for the α chain specific for the CNTF receptor was detected by Northern blot in tumors secreting prolactin (PRL) and GH and in non-functioning tumors. We found that both IL-11 and CNTF exerted a similar stimulatory effect on GH mRNA expression in somatotropic monolayer cell cultures from acromegalic tumors, but these cytokines had no significant influence on GH secretion. CNTF stimulates prolactin secretion in lactotropic monolayer cell cultures from patients with prolactinoma. In monolayer cell cultures from normal rat anterior pituitary, IL-11 and CNTF had no significant effect on the release of either GH or PRL, or on GH mRNA. However, when the cells were cultured in aggregate cultures, in which the three-dimensional structure of the cells is reconstituted, both cytokines, in doses at which they had no effect on monolayer cultures, significantly stimulated both PRL and GH secretion. These data show that IL-11 and CNTF may act as regulatory factors in anterior pituitary cells, in which the three-dimensional structure of the gland is of critical importance. |
| title |
Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| title_short |
Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| title_full |
Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| title_fullStr |
Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| title_full_unstemmed |
Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures |
| title_sort |
effects of the gp130 cytokines ciliary neurotropic factor (cntf) and interleukin-11 on pituitary cells: cntf receptors on human pituitary adenomas and stimulation of prolactin and gh secretion in normal rat anterior pituitary aggregate cultures |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v169_n3_p539_PerezCastro http://hdl.handle.net/20.500.12110/paper_00220795_v169_n3_p539_PerezCastro |
| _version_ |
1768541878581460992 |