Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cell...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v160_n3_p483_Gloddek http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek |
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paper:paper_00220795_v160_n3_p483_Gloddek2023-06-08T14:45:25Z Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells dexamethasone glucocorticoid hypophysis adenylate cyclase activating polypeptide interleukin 6 mifepristone vasculotropin angiogenesis animal cell article blood vessel permeability cell function cell growth endothelium cell hormonal regulation hormone blood level hormone release inhibition kinetics nonhuman priority journal protein expression rat receptor upregulation regulatory mechanism There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cells. In the normal anterior pituitary, VEGF has been detected only in folliculostellate (FS) cells. In the present study, the regulation of the release of VEGF from FS-like mouse TtT/GF cells, and from FS cells of rat pituitary monolayer cell cultures was investigated using a specific VEGF ELISA. Basal release of VEGF was demonstrated in cultures of both TtT/GF cells and rat pituitary cells. Interestingly, the VEGF secretion was stimulated by both forms of pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27), indicating that this hypothalamic peptide regulates endothelial cell function and growth within the pituitary. VEGF secretion was also stimulated by interleukin-6 (IL-6) whereas basal, IL- 6- and PACAP-stimulated secretion was inhibited by the synthetic glucocorticoid dexamethasone. The inhibitory action of dexamethasone was reversed by the glucocorticoid receptor antagonist RU486, suggesting that in FS cells functional glucocorticoid receptors mediate the inhibitory action of glucocorticoids on the VEGF secretion. The endocrine and auto-/paracrine control of VEGF production in pituitary FS cells by PACAP, IL-6 and glucocorticoids may play an important role both in angiogenesis and vascular permeability regulation within the pituitary under physiological and pathophysiological conditions. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v160_n3_p483_Gloddek http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dexamethasone glucocorticoid hypophysis adenylate cyclase activating polypeptide interleukin 6 mifepristone vasculotropin angiogenesis animal cell article blood vessel permeability cell function cell growth endothelium cell hormonal regulation hormone blood level hormone release inhibition kinetics nonhuman priority journal protein expression rat receptor upregulation regulatory mechanism |
| spellingShingle |
dexamethasone glucocorticoid hypophysis adenylate cyclase activating polypeptide interleukin 6 mifepristone vasculotropin angiogenesis animal cell article blood vessel permeability cell function cell growth endothelium cell hormonal regulation hormone blood level hormone release inhibition kinetics nonhuman priority journal protein expression rat receptor upregulation regulatory mechanism Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| topic_facet |
dexamethasone glucocorticoid hypophysis adenylate cyclase activating polypeptide interleukin 6 mifepristone vasculotropin angiogenesis animal cell article blood vessel permeability cell function cell growth endothelium cell hormonal regulation hormone blood level hormone release inhibition kinetics nonhuman priority journal protein expression rat receptor upregulation regulatory mechanism |
| description |
There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cells. In the normal anterior pituitary, VEGF has been detected only in folliculostellate (FS) cells. In the present study, the regulation of the release of VEGF from FS-like mouse TtT/GF cells, and from FS cells of rat pituitary monolayer cell cultures was investigated using a specific VEGF ELISA. Basal release of VEGF was demonstrated in cultures of both TtT/GF cells and rat pituitary cells. Interestingly, the VEGF secretion was stimulated by both forms of pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27), indicating that this hypothalamic peptide regulates endothelial cell function and growth within the pituitary. VEGF secretion was also stimulated by interleukin-6 (IL-6) whereas basal, IL- 6- and PACAP-stimulated secretion was inhibited by the synthetic glucocorticoid dexamethasone. The inhibitory action of dexamethasone was reversed by the glucocorticoid receptor antagonist RU486, suggesting that in FS cells functional glucocorticoid receptors mediate the inhibitory action of glucocorticoids on the VEGF secretion. The endocrine and auto-/paracrine control of VEGF production in pituitary FS cells by PACAP, IL-6 and glucocorticoids may play an important role both in angiogenesis and vascular permeability regulation within the pituitary under physiological and pathophysiological conditions. |
| title |
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| title_short |
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| title_full |
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| title_fullStr |
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| title_full_unstemmed |
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| title_sort |
pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v160_n3_p483_Gloddek http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek |
| _version_ |
1768543309943275520 |