Cuprizone-Induced demyelination in CNP::GFP transgenic mice
Cuprizone (bis-cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′-3′-cyclic nucle...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219967_v518_n12_p2261_Silvestroff http://hdl.handle.net/20.500.12110/paper_00219967_v518_n12_p2261_Silvestroff |
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paper:paper_00219967_v518_n12_p2261_Silvestroff2023-06-08T14:45:00Z Cuprizone-Induced demyelination in CNP::GFP transgenic mice Cuprizone Demyelination Oligodendrocytes Remyelination 2',3' cyclic nucleotide 3' phosphodiesterase cuprizone green fluorescent protein myelin basic protein adult animal animal cell animal cell culture animal experiment animal model animal tissue anterior commissure antibody specificity article astrocytosis brain cortex brain stem caudate nucleus cell activation cell damage central nervous system controlled study corpus callosum demyelination hippocampus immunohistochemistry microglia microphotography microscopy mouse nonhuman olfactory bulb oligodendroglia optic chiasm priority journal protein expression putamen quantitative analysis tissue section transgenic mouse 2',3'-Cyclic-Nucleotide Phosphodiesterases Animals Astrocytes Brain Cell Count Cell Proliferation Cuprizone Demyelinating Diseases Glial Fibrillary Acidic Protein Gliosis Green Fluorescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Microglia Myelin Basic Proteins Neurons Oligodendroglia Promoter Regions, Genetic Cuprizone (bis-cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′-3′-cyclic nucleotide 3′-phosphodies-terase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well-known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone-treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. © 2010 Wiley-Liss, Inc. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219967_v518_n12_p2261_Silvestroff http://hdl.handle.net/20.500.12110/paper_00219967_v518_n12_p2261_Silvestroff |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cuprizone Demyelination Oligodendrocytes Remyelination 2',3' cyclic nucleotide 3' phosphodiesterase cuprizone green fluorescent protein myelin basic protein adult animal animal cell animal cell culture animal experiment animal model animal tissue anterior commissure antibody specificity article astrocytosis brain cortex brain stem caudate nucleus cell activation cell damage central nervous system controlled study corpus callosum demyelination hippocampus immunohistochemistry microglia microphotography microscopy mouse nonhuman olfactory bulb oligodendroglia optic chiasm priority journal protein expression putamen quantitative analysis tissue section transgenic mouse 2',3'-Cyclic-Nucleotide Phosphodiesterases Animals Astrocytes Brain Cell Count Cell Proliferation Cuprizone Demyelinating Diseases Glial Fibrillary Acidic Protein Gliosis Green Fluorescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Microglia Myelin Basic Proteins Neurons Oligodendroglia Promoter Regions, Genetic |
| spellingShingle |
Cuprizone Demyelination Oligodendrocytes Remyelination 2',3' cyclic nucleotide 3' phosphodiesterase cuprizone green fluorescent protein myelin basic protein adult animal animal cell animal cell culture animal experiment animal model animal tissue anterior commissure antibody specificity article astrocytosis brain cortex brain stem caudate nucleus cell activation cell damage central nervous system controlled study corpus callosum demyelination hippocampus immunohistochemistry microglia microphotography microscopy mouse nonhuman olfactory bulb oligodendroglia optic chiasm priority journal protein expression putamen quantitative analysis tissue section transgenic mouse 2',3'-Cyclic-Nucleotide Phosphodiesterases Animals Astrocytes Brain Cell Count Cell Proliferation Cuprizone Demyelinating Diseases Glial Fibrillary Acidic Protein Gliosis Green Fluorescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Microglia Myelin Basic Proteins Neurons Oligodendroglia Promoter Regions, Genetic Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| topic_facet |
Cuprizone Demyelination Oligodendrocytes Remyelination 2',3' cyclic nucleotide 3' phosphodiesterase cuprizone green fluorescent protein myelin basic protein adult animal animal cell animal cell culture animal experiment animal model animal tissue anterior commissure antibody specificity article astrocytosis brain cortex brain stem caudate nucleus cell activation cell damage central nervous system controlled study corpus callosum demyelination hippocampus immunohistochemistry microglia microphotography microscopy mouse nonhuman olfactory bulb oligodendroglia optic chiasm priority journal protein expression putamen quantitative analysis tissue section transgenic mouse 2',3'-Cyclic-Nucleotide Phosphodiesterases Animals Astrocytes Brain Cell Count Cell Proliferation Cuprizone Demyelinating Diseases Glial Fibrillary Acidic Protein Gliosis Green Fluorescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Microglia Myelin Basic Proteins Neurons Oligodendroglia Promoter Regions, Genetic |
| description |
Cuprizone (bis-cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′-3′-cyclic nucleotide 3′-phosphodies-terase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well-known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone-treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. © 2010 Wiley-Liss, Inc. |
| title |
Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| title_short |
Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| title_full |
Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| title_fullStr |
Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| title_full_unstemmed |
Cuprizone-Induced demyelination in CNP::GFP transgenic mice |
| title_sort |
cuprizone-induced demyelination in cnp::gfp transgenic mice |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219967_v518_n12_p2261_Silvestroff http://hdl.handle.net/20.500.12110/paper_00219967_v518_n12_p2261_Silvestroff |
| _version_ |
1768545128514846720 |