Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum

Fusarium oxysporum L11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. In this study, reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to different atmospheric pressure ionization sources-quadrupole-t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cirigliano, Adriana Mónica, Rodríguez, María Alejandra, Bertinetti, Brenda Verónica, Godeas, Alicia Margarita, Cabrera, Gabriela Myriam
Publicado:	2016
Materias:	Cyclosporin A LC/MS Metabolic profiling Metal post-column addition Sansalvamide Atmospheric chemistry Atmospheric ionization Atmospheric pressure Binding energy Chromatography Column chromatography Copper Drug products Electrospray ionization Fungi High performance liquid chromatography Ionization Ionization of liquids Liquid chromatography Mass spectrometry Metabolism Metabolites Metal ions Metals Peptides Spectrometry Zinc compounds Metal posts Extraction chemical compound copper cyclosporin derivative depsipeptide ergosteroid fatty acid fatty acid derivative piperazinedione sansalvamide derivative sodium chloride steroid unclassified drug zinc calcium chloride chloride copper sulfate lipid zinc chloride zinc derivative analytic method Article atmospheric pressure chemical ionization atmospheric pressure photoionization controlled study electrospray Fusarium oxysporum ionization measurement accuracy metabolite metal binding molecular weight nonhuman phase separation priority journal quadrupole mass spectrometry reversed phase high performance liquid chromatography structure analysis time of flight mass spectrometry atmospheric pressure chemistry Fusarium high performance liquid chromatography metabolism mycelium procedures reversed phase liquid chromatography tandem mass spectrometry Atmospheric Pressure Calcium Chloride Chlorides Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Copper Sulfate Cyclosporins Depsipeptides Diketopiperazines Lipids Mycelium Steroids Tandem Mass Spectrometry Zinc Compounds
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v1439_n_p97_Cirigliano http://hdl.handle.net/20.500.12110/paper_00219673_v1439_n_p97_Cirigliano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219673_v1439_n_p97_Cirigliano
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cyclosporin A LC/MS Metabolic profiling Metal post-column addition Sansalvamide Atmospheric chemistry Atmospheric ionization Atmospheric pressure Binding energy Chromatography Column chromatography Copper Drug products Electrospray ionization Fungi High performance liquid chromatography Ionization Ionization of liquids Liquid chromatography Mass spectrometry Metabolism Metabolites Metal ions Metals Peptides Spectrometry Zinc compounds Cyclosporin A LC/MS Metabolic profiling Metal posts Sansalvamide Extraction chemical compound copper cyclosporin derivative depsipeptide ergosteroid fatty acid fatty acid derivative piperazinedione sansalvamide derivative sodium chloride steroid unclassified drug zinc calcium chloride chloride copper sulfate cyclosporin derivative depsipeptide lipid piperazinedione steroid zinc chloride zinc derivative analytic method Article atmospheric pressure chemical ionization atmospheric pressure photoionization controlled study electrospray Fusarium oxysporum ionization measurement accuracy metabolite metal binding molecular weight nonhuman phase separation priority journal quadrupole mass spectrometry reversed phase high performance liquid chromatography structure analysis time of flight mass spectrometry atmospheric pressure chemistry Fusarium high performance liquid chromatography metabolism mycelium procedures reversed phase liquid chromatography tandem mass spectrometry Atmospheric Pressure Calcium Chloride Chlorides Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Copper Sulfate Cyclosporins Depsipeptides Diketopiperazines Fusarium Lipids Mycelium Steroids Tandem Mass Spectrometry Zinc Compounds
spellingShingle	Cyclosporin A LC/MS Metabolic profiling Metal post-column addition Sansalvamide Atmospheric chemistry Atmospheric ionization Atmospheric pressure Binding energy Chromatography Column chromatography Copper Drug products Electrospray ionization Fungi High performance liquid chromatography Ionization Ionization of liquids Liquid chromatography Mass spectrometry Metabolism Metabolites Metal ions Metals Peptides Spectrometry Zinc compounds Cyclosporin A LC/MS Metabolic profiling Metal posts Sansalvamide Extraction chemical compound copper cyclosporin derivative depsipeptide ergosteroid fatty acid fatty acid derivative piperazinedione sansalvamide derivative sodium chloride steroid unclassified drug zinc calcium chloride chloride copper sulfate cyclosporin derivative depsipeptide lipid piperazinedione steroid zinc chloride zinc derivative analytic method Article atmospheric pressure chemical ionization atmospheric pressure photoionization controlled study electrospray Fusarium oxysporum ionization measurement accuracy metabolite metal binding molecular weight nonhuman phase separation priority journal quadrupole mass spectrometry reversed phase high performance liquid chromatography structure analysis time of flight mass spectrometry atmospheric pressure chemistry Fusarium high performance liquid chromatography metabolism mycelium procedures reversed phase liquid chromatography tandem mass spectrometry Atmospheric Pressure Calcium Chloride Chlorides Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Copper Sulfate Cyclosporins Depsipeptides Diketopiperazines Fusarium Lipids Mycelium Steroids Tandem Mass Spectrometry Zinc Compounds Cirigliano, Adriana Mónica Rodríguez, María Alejandra Bertinetti, Brenda Verónica Godeas, Alicia Margarita Cabrera, Gabriela Myriam Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
topic_facet	Cyclosporin A LC/MS Metabolic profiling Metal post-column addition Sansalvamide Atmospheric chemistry Atmospheric ionization Atmospheric pressure Binding energy Chromatography Column chromatography Copper Drug products Electrospray ionization Fungi High performance liquid chromatography Ionization Ionization of liquids Liquid chromatography Mass spectrometry Metabolism Metabolites Metal ions Metals Peptides Spectrometry Zinc compounds Cyclosporin A LC/MS Metabolic profiling Metal posts Sansalvamide Extraction chemical compound copper cyclosporin derivative depsipeptide ergosteroid fatty acid fatty acid derivative piperazinedione sansalvamide derivative sodium chloride steroid unclassified drug zinc calcium chloride chloride copper sulfate cyclosporin derivative depsipeptide lipid piperazinedione steroid zinc chloride zinc derivative analytic method Article atmospheric pressure chemical ionization atmospheric pressure photoionization controlled study electrospray Fusarium oxysporum ionization measurement accuracy metabolite metal binding molecular weight nonhuman phase separation priority journal quadrupole mass spectrometry reversed phase high performance liquid chromatography structure analysis time of flight mass spectrometry atmospheric pressure chemistry Fusarium high performance liquid chromatography metabolism mycelium procedures reversed phase liquid chromatography tandem mass spectrometry Atmospheric Pressure Calcium Chloride Chlorides Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Copper Sulfate Cyclosporins Depsipeptides Diketopiperazines Fusarium Lipids Mycelium Steroids Tandem Mass Spectrometry Zinc Compounds
description	Fusarium oxysporum L11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. In this study, reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (API-QTOF-MS) was applied for the comprehensive profiling of the metabolites from the extract. The employed sources were electrospray (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). Post-column addition of metal solutions of Ca, Cu and Zn(II) was also tested using ESI.A total of 137 compounds were identified or tentatively identified by matching their accurate mass signals, suggested molecular formulae and MS/MS analysis with previously reported data. Some compounds were isolated and identified by NMR. The extract was rich in cyclic peptides like cyclosporins, diketopiperazines and sansalvamides, most of which were new, and are reported here for the first time. The use of post-column addition of metals resulted in a useful strategy for the discrimination of compound classes since specific adducts were observed for the different compound families. This technique also allowed the screening for compounds with metal binding properties. Thus, the applied methodology is a useful choice for the metabolic profiling of extracts and also for the selection of metabolites with potential biological activities related to interactions with metal ions. © 2015 Elsevier B.V.
author	Cirigliano, Adriana Mónica Rodríguez, María Alejandra Bertinetti, Brenda Verónica Godeas, Alicia Margarita Cabrera, Gabriela Myriam
author_facet	Cirigliano, Adriana Mónica Rodríguez, María Alejandra Bertinetti, Brenda Verónica Godeas, Alicia Margarita Cabrera, Gabriela Myriam
author_sort	Cirigliano, Adriana Mónica
title	Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
title_short	Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
title_full	Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
title_fullStr	Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
title_full_unstemmed	Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum
title_sort	liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of fusarium oxysporum
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v1439_n_p97_Cirigliano http://hdl.handle.net/20.500.12110/paper_00219673_v1439_n_p97_Cirigliano
work_keys_str_mv	AT ciriglianoadrianamonica liquidchromatographycoupledtodifferentatmosphericpressureionizationsourcesquadrupoletimeofflightmassspectrometryandpostcolumnadditionofmetalsaltsolutionsasapowerfultoolforthemetabolicprofilingoffusariumoxysporum AT rodriguezmariaalejandra liquidchromatographycoupledtodifferentatmosphericpressureionizationsourcesquadrupoletimeofflightmassspectrometryandpostcolumnadditionofmetalsaltsolutionsasapowerfultoolforthemetabolicprofilingoffusariumoxysporum AT bertinettibrendaveronica liquidchromatographycoupledtodifferentatmosphericpressureionizationsourcesquadrupoletimeofflightmassspectrometryandpostcolumnadditionofmetalsaltsolutionsasapowerfultoolforthemetabolicprofilingoffusariumoxysporum AT godeasaliciamargarita liquidchromatographycoupledtodifferentatmosphericpressureionizationsourcesquadrupoletimeofflightmassspectrometryandpostcolumnadditionofmetalsaltsolutionsasapowerfultoolforthemetabolicprofilingoffusariumoxysporum AT cabreragabrielamyriam liquidchromatographycoupledtodifferentatmosphericpressureionizationsourcesquadrupoletimeofflightmassspectrometryandpostcolumnadditionofmetalsaltsolutionsasapowerfultoolforthemetabolicprofilingoffusariumoxysporum
_version_	1768544896763822080
spelling	paper:paper_00219673_v1439_n_p97_Cirigliano2023-06-08T14:44:42Z Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum Cirigliano, Adriana Mónica Rodríguez, María Alejandra Bertinetti, Brenda Verónica Godeas, Alicia Margarita Cabrera, Gabriela Myriam Cyclosporin A LC/MS Metabolic profiling Metal post-column addition Sansalvamide Atmospheric chemistry Atmospheric ionization Atmospheric pressure Binding energy Chromatography Column chromatography Copper Drug products Electrospray ionization Fungi High performance liquid chromatography Ionization Ionization of liquids Liquid chromatography Mass spectrometry Metabolism Metabolites Metal ions Metals Peptides Spectrometry Zinc compounds Cyclosporin A LC/MS Metabolic profiling Metal posts Sansalvamide Extraction chemical compound copper cyclosporin derivative depsipeptide ergosteroid fatty acid fatty acid derivative piperazinedione sansalvamide derivative sodium chloride steroid unclassified drug zinc calcium chloride chloride copper sulfate cyclosporin derivative depsipeptide lipid piperazinedione steroid zinc chloride zinc derivative analytic method Article atmospheric pressure chemical ionization atmospheric pressure photoionization controlled study electrospray Fusarium oxysporum ionization measurement accuracy metabolite metal binding molecular weight nonhuman phase separation priority journal quadrupole mass spectrometry reversed phase high performance liquid chromatography structure analysis time of flight mass spectrometry atmospheric pressure chemistry Fusarium high performance liquid chromatography metabolism mycelium procedures reversed phase liquid chromatography tandem mass spectrometry Atmospheric Pressure Calcium Chloride Chlorides Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Copper Sulfate Cyclosporins Depsipeptides Diketopiperazines Fusarium Lipids Mycelium Steroids Tandem Mass Spectrometry Zinc Compounds Fusarium oxysporum L11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. In this study, reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (API-QTOF-MS) was applied for the comprehensive profiling of the metabolites from the extract. The employed sources were electrospray (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). Post-column addition of metal solutions of Ca, Cu and Zn(II) was also tested using ESI.A total of 137 compounds were identified or tentatively identified by matching their accurate mass signals, suggested molecular formulae and MS/MS analysis with previously reported data. Some compounds were isolated and identified by NMR. The extract was rich in cyclic peptides like cyclosporins, diketopiperazines and sansalvamides, most of which were new, and are reported here for the first time. The use of post-column addition of metals resulted in a useful strategy for the discrimination of compound classes since specific adducts were observed for the different compound families. This technique also allowed the screening for compounds with metal binding properties. Thus, the applied methodology is a useful choice for the metabolic profiling of extracts and also for the selection of metabolites with potential biological activities related to interactions with metal ions. © 2015 Elsevier B.V. Fil:Cirigliano, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertinetti, B.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cabrera, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v1439_n_p97_Cirigliano http://hdl.handle.net/20.500.12110/paper_00219673_v1439_n_p97_Cirigliano

Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum

Ejemplares similares