p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage
The stress protein p8 is a small, highly basic, unfolded, and multifunctional protein.Wehave previously shown that most of its functions are exerted through interactions with other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanis...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v221_n3_p594_Gironella http://hdl.handle.net/20.500.12110/paper_00219541_v221_n3_p594_Gironella |
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paper:paper_00219541_v221_n3_p594_Gironella2023-06-08T14:43:48Z p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage double stranded DNA histone acetyltransferase protein p8 basic helix loop helix transcription factor histone histone acetyltransferase MSL1 protein, human nuclear protein P8 protein, human recombinant protein signal peptide small interfering RNA TP53BP1 protein, human tumor protein article chromatin controlled study DNA damage DNA repair down regulation female gamma irradiation human human cell priority journal protein binding protein expression protein protein interaction cell line cell proliferation chemistry clonogenic assay double stranded DNA break gamma radiation gene expression genetic transfection genetics HeLa cell immunoprecipitation metabolism physiology radiation exposure surface plasmon resonance two hybrid system Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Colony-Forming Units Assay DNA Breaks, Double-Stranded DNA Repair Gamma Rays Gene Expression Hela Cells Histone Acetyltransferases Histones Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins Neoplasm Proteins Nuclear Proteins Protein Binding Recombinant Proteins RNA, Small Interfering Surface Plasmon Resonance Transfection Two-Hybrid System Techniques The stress protein p8 is a small, highly basic, unfolded, and multifunctional protein.Wehave previously shown that most of its functions are exerted through interactions with other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanism, by which p8 binds and negatively regulates MSL1, a histone acetyl transferase (HAT)-associated protein, which in turn binds the DNA-damage-associated 53BP1 protein to facilitate DNA repair following DNA γ-irradiation. Contrary to the HAT-associated activity, MSL1-dependent DNA-repair activity is almost completely dependent on 53BP1 expression. The picture that has emerged from our findings is that 53BP1 could be a scaffold that gets the HAT MSL1-dependent DNA-repair activity to the sites of DNA damage. Finally, we also found that, although p8 expression is transiently activated after γ-irradiation, it is eventually submitted to sustained down-regulation, presumably to allow development of MSL1-associated DNA-repair activity. Weconclude that interaction of MSL1 with 53BP1 brings MSL1-dependent HAT activity to the vicinity of damaged DNA. MSL1-dependent HAT activity, which is negatively regulated by the stress protein p8, induces chromatin remodeling and relaxation allowing access to DNA of the repair machinery. © 2009 Wiley-Liss, Inc. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v221_n3_p594_Gironella http://hdl.handle.net/20.500.12110/paper_00219541_v221_n3_p594_Gironella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
double stranded DNA histone acetyltransferase protein p8 basic helix loop helix transcription factor histone histone acetyltransferase MSL1 protein, human nuclear protein P8 protein, human recombinant protein signal peptide small interfering RNA TP53BP1 protein, human tumor protein article chromatin controlled study DNA damage DNA repair down regulation female gamma irradiation human human cell priority journal protein binding protein expression protein protein interaction cell line cell proliferation chemistry clonogenic assay double stranded DNA break gamma radiation gene expression genetic transfection genetics HeLa cell immunoprecipitation metabolism physiology radiation exposure surface plasmon resonance two hybrid system Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Colony-Forming Units Assay DNA Breaks, Double-Stranded DNA Repair Gamma Rays Gene Expression Hela Cells Histone Acetyltransferases Histones Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins Neoplasm Proteins Nuclear Proteins Protein Binding Recombinant Proteins RNA, Small Interfering Surface Plasmon Resonance Transfection Two-Hybrid System Techniques |
| spellingShingle |
double stranded DNA histone acetyltransferase protein p8 basic helix loop helix transcription factor histone histone acetyltransferase MSL1 protein, human nuclear protein P8 protein, human recombinant protein signal peptide small interfering RNA TP53BP1 protein, human tumor protein article chromatin controlled study DNA damage DNA repair down regulation female gamma irradiation human human cell priority journal protein binding protein expression protein protein interaction cell line cell proliferation chemistry clonogenic assay double stranded DNA break gamma radiation gene expression genetic transfection genetics HeLa cell immunoprecipitation metabolism physiology radiation exposure surface plasmon resonance two hybrid system Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Colony-Forming Units Assay DNA Breaks, Double-Stranded DNA Repair Gamma Rays Gene Expression Hela Cells Histone Acetyltransferases Histones Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins Neoplasm Proteins Nuclear Proteins Protein Binding Recombinant Proteins RNA, Small Interfering Surface Plasmon Resonance Transfection Two-Hybrid System Techniques p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| topic_facet |
double stranded DNA histone acetyltransferase protein p8 basic helix loop helix transcription factor histone histone acetyltransferase MSL1 protein, human nuclear protein P8 protein, human recombinant protein signal peptide small interfering RNA TP53BP1 protein, human tumor protein article chromatin controlled study DNA damage DNA repair down regulation female gamma irradiation human human cell priority journal protein binding protein expression protein protein interaction cell line cell proliferation chemistry clonogenic assay double stranded DNA break gamma radiation gene expression genetic transfection genetics HeLa cell immunoprecipitation metabolism physiology radiation exposure surface plasmon resonance two hybrid system Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Colony-Forming Units Assay DNA Breaks, Double-Stranded DNA Repair Gamma Rays Gene Expression Hela Cells Histone Acetyltransferases Histones Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins Neoplasm Proteins Nuclear Proteins Protein Binding Recombinant Proteins RNA, Small Interfering Surface Plasmon Resonance Transfection Two-Hybrid System Techniques |
| description |
The stress protein p8 is a small, highly basic, unfolded, and multifunctional protein.Wehave previously shown that most of its functions are exerted through interactions with other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanism, by which p8 binds and negatively regulates MSL1, a histone acetyl transferase (HAT)-associated protein, which in turn binds the DNA-damage-associated 53BP1 protein to facilitate DNA repair following DNA γ-irradiation. Contrary to the HAT-associated activity, MSL1-dependent DNA-repair activity is almost completely dependent on 53BP1 expression. The picture that has emerged from our findings is that 53BP1 could be a scaffold that gets the HAT MSL1-dependent DNA-repair activity to the sites of DNA damage. Finally, we also found that, although p8 expression is transiently activated after γ-irradiation, it is eventually submitted to sustained down-regulation, presumably to allow development of MSL1-associated DNA-repair activity. Weconclude that interaction of MSL1 with 53BP1 brings MSL1-dependent HAT activity to the vicinity of damaged DNA. MSL1-dependent HAT activity, which is negatively regulated by the stress protein p8, induces chromatin remodeling and relaxation allowing access to DNA of the repair machinery. © 2009 Wiley-Liss, Inc. |
| title |
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| title_short |
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| title_full |
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| title_fullStr |
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| title_full_unstemmed |
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage |
| title_sort |
p8/nupr1 regulates dna-repair activity after double-strand gamma irradiation-induced dna damage |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v221_n3_p594_Gironella http://hdl.handle.net/20.500.12110/paper_00219541_v221_n3_p594_Gironella |
| _version_ |
1768544762996981760 |