A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii
Background: Rhomboid proteases are ubiquitous, and their role in Archaea has not been explored. Results: We generated a rhomboid deletion mutant that displayed a glycosylation defect. Conclusion: Deletion of a rhomboid protease gene altered S-layer glycoprotein N-glycosylation. Significance: This wo...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v289_n16_p11304_Parente http://hdl.handle.net/20.500.12110/paper_00219258_v289_n16_p11304_Parente |
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paper:paper_00219258_v289_n16_p11304_Parente2023-06-08T14:43:35Z A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii Esterification Genes Glycosylation Oligosaccharides Deletion mutants Gene deletion Haloferax volcanii N-Glycosylation N-linked Protein glycosylation S-layers Structural characterization Glycoproteins glycoprotein oligosaccharide proteinase RhoII protease unclassified drug article bacterial gene bacterial strain bacterium mutant carbohydrate analysis controlled study enzyme activity gene deletion Haloferax volcanii high performance liquid chromatography mass spectrometry nonhuman priority journal protein glycosylation RhoII gene structure analysis Archaea Glycoprotein Glycosylation Halophiles Mass Spectrometry (MS) Rhomboid Protease Endopeptidases Gene Knockdown Techniques Glycosylation Haloferax volcanii Membrane Glycoproteins Oligosaccharides Background: Rhomboid proteases are ubiquitous, and their role in Archaea has not been explored. Results: We generated a rhomboid deletion mutant that displayed a glycosylation defect. Conclusion: Deletion of a rhomboid protease gene altered S-layer glycoprotein N-glycosylation. Significance: This work provides structural characterization of a novel oligosaccharide bound to H. volcanii S-layer glycoprotein and relates a rhomboid protease with the protein glycosylation process. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v289_n16_p11304_Parente http://hdl.handle.net/20.500.12110/paper_00219258_v289_n16_p11304_Parente |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Esterification Genes Glycosylation Oligosaccharides Deletion mutants Gene deletion Haloferax volcanii N-Glycosylation N-linked Protein glycosylation S-layers Structural characterization Glycoproteins glycoprotein oligosaccharide proteinase RhoII protease unclassified drug article bacterial gene bacterial strain bacterium mutant carbohydrate analysis controlled study enzyme activity gene deletion Haloferax volcanii high performance liquid chromatography mass spectrometry nonhuman priority journal protein glycosylation RhoII gene structure analysis Archaea Glycoprotein Glycosylation Halophiles Mass Spectrometry (MS) Rhomboid Protease Endopeptidases Gene Knockdown Techniques Glycosylation Haloferax volcanii Membrane Glycoproteins Oligosaccharides |
| spellingShingle |
Esterification Genes Glycosylation Oligosaccharides Deletion mutants Gene deletion Haloferax volcanii N-Glycosylation N-linked Protein glycosylation S-layers Structural characterization Glycoproteins glycoprotein oligosaccharide proteinase RhoII protease unclassified drug article bacterial gene bacterial strain bacterium mutant carbohydrate analysis controlled study enzyme activity gene deletion Haloferax volcanii high performance liquid chromatography mass spectrometry nonhuman priority journal protein glycosylation RhoII gene structure analysis Archaea Glycoprotein Glycosylation Halophiles Mass Spectrometry (MS) Rhomboid Protease Endopeptidases Gene Knockdown Techniques Glycosylation Haloferax volcanii Membrane Glycoproteins Oligosaccharides A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| topic_facet |
Esterification Genes Glycosylation Oligosaccharides Deletion mutants Gene deletion Haloferax volcanii N-Glycosylation N-linked Protein glycosylation S-layers Structural characterization Glycoproteins glycoprotein oligosaccharide proteinase RhoII protease unclassified drug article bacterial gene bacterial strain bacterium mutant carbohydrate analysis controlled study enzyme activity gene deletion Haloferax volcanii high performance liquid chromatography mass spectrometry nonhuman priority journal protein glycosylation RhoII gene structure analysis Archaea Glycoprotein Glycosylation Halophiles Mass Spectrometry (MS) Rhomboid Protease Endopeptidases Gene Knockdown Techniques Glycosylation Haloferax volcanii Membrane Glycoproteins Oligosaccharides |
| description |
Background: Rhomboid proteases are ubiquitous, and their role in Archaea has not been explored. Results: We generated a rhomboid deletion mutant that displayed a glycosylation defect. Conclusion: Deletion of a rhomboid protease gene altered S-layer glycoprotein N-glycosylation. Significance: This work provides structural characterization of a novel oligosaccharide bound to H. volcanii S-layer glycoprotein and relates a rhomboid protease with the protein glycosylation process. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. |
| title |
A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| title_short |
A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| title_full |
A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| title_fullStr |
A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| title_full_unstemmed |
A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii |
| title_sort |
rhomboid protease gene deletion affects a novel oligosaccharide n-linked to the s-layer glycoprotein of haloferax volcanii |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v289_n16_p11304_Parente http://hdl.handle.net/20.500.12110/paper_00219258_v289_n16_p11304_Parente |
| _version_ |
1768546005974777856 |