Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation

The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cycli...

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Autores principales:	Bernath, Viviana A., Muro, Andrés Fernando, Vitullo, Alfredo Daniel, Bley, Miguel Andrés, Barañao, José Lino S., Kornblihtt, Alberto Rodolfo
Publicado:	1990
Materias:	cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v265_n30_p18219_Bernath http://hdl.handle.net/20.500.12110/paper_00219258_v265_n30_p18219_Bernath
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219258_v265_n30_p18219_Bernath
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spelling	paper:paper_00219258_v265_n30_p18219_Bernath2023-06-08T14:43:17Z Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation Bernath, Viviana A. Muro, Andrés Fernando Vitullo, Alfredo Daniel Bley, Miguel Andrés Barañao, José Lino S. Kornblihtt, Alberto Rodolfo cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cyclic AMP-dependent phosphorylations. Cyclic AMP stimulates transcription of genes that carry the cAMP-responsive element (CRE,5'TGACGTCA3') in their promoters. The fibronectin (FN) gene contains one CRE sequence at position -170. However, gonadotropins and cAMP inhibit FN gene expression in granulosa cells. To study the mechanism of the inhibition we developed a bovine granulosa cell line (BGC-1) that synthesizes estradiol in response to FSH and in which FSH and dibutyryl cAMP specifically decrease FN synthesis and its mRNA levels. The inhibitory effect (a) is not due to an alternation in FN mRNA stability, (b) requires upstream sequences other than CRE, located between positions -510 and -223, that are able to bind granulosa cell nuclear proteins, (c) is entirely dependent on the synthesis of intermediate proteins induced and or phosphorylated by cAMP, and (d) effectively suppresses the CRE-dependent transcriptional activation. Fil:Bernath, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vitullo, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bley, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v265_n30_p18219_Bernath http://hdl.handle.net/20.500.12110/paper_00219258_v265_n30_p18219_Bernath
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae
spellingShingle	cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae Bernath, Viviana A. Muro, Andrés Fernando Vitullo, Alfredo Daniel Bley, Miguel Andrés Barañao, José Lino S. Kornblihtt, Alberto Rodolfo Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
topic_facet	cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae
description	The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cyclic AMP-dependent phosphorylations. Cyclic AMP stimulates transcription of genes that carry the cAMP-responsive element (CRE,5'TGACGTCA3') in their promoters. The fibronectin (FN) gene contains one CRE sequence at position -170. However, gonadotropins and cAMP inhibit FN gene expression in granulosa cells. To study the mechanism of the inhibition we developed a bovine granulosa cell line (BGC-1) that synthesizes estradiol in response to FSH and in which FSH and dibutyryl cAMP specifically decrease FN synthesis and its mRNA levels. The inhibitory effect (a) is not due to an alternation in FN mRNA stability, (b) requires upstream sequences other than CRE, located between positions -510 and -223, that are able to bind granulosa cell nuclear proteins, (c) is entirely dependent on the synthesis of intermediate proteins induced and or phosphorylated by cAMP, and (d) effectively suppresses the CRE-dependent transcriptional activation.
author	Bernath, Viviana A. Muro, Andrés Fernando Vitullo, Alfredo Daniel Bley, Miguel Andrés Barañao, José Lino S. Kornblihtt, Alberto Rodolfo
author_facet	Bernath, Viviana A. Muro, Andrés Fernando Vitullo, Alfredo Daniel Bley, Miguel Andrés Barañao, José Lino S. Kornblihtt, Alberto Rodolfo
author_sort	Bernath, Viviana A.
title	Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
title_short	Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
title_full	Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
title_fullStr	Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
title_full_unstemmed	Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
title_sort	cyclic amp inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses camp-responsive element-dependent transcriptional activation
publishDate	1990
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v265_n30_p18219_Bernath http://hdl.handle.net/20.500.12110/paper_00219258_v265_n30_p18219_Bernath
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Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation

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