Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence

Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose...

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Autores principales:	Katzen, Federico, Ferreiro, Diego U., Ielmini, María Verónica, Ielpi, Luis
Publicado:	1998
Materias:	gene product glucose 1 phosphate glucuronic acid lipid mannose pentasaccharide phosphate protein transferase xanthan article bacterial virulence biosynthesis carboxy terminal sequence gene gene function mutant nonhuman nucleotide sequence priority journal xanthomonas campestris Base Sequence Genes, Bacterial Molecular Sequence Data Mutation Plant Diseases Polysaccharides, Bacterial Uridine Diphosphate Glucose Virulence Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen http://hdl.handle.net/20.500.12110/paper_00219193_v180_n7_p1607_Katzen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219193_v180_n7_p1607_Katzen
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spelling	paper:paper_00219193_v180_n7_p1607_Katzen2023-06-08T14:43:10Z Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence Katzen, Federico Ferreiro, Diego U. Ielmini, María Verónica Ielpi, Luis gene product glucose 1 phosphate glucuronic acid lipid mannose pentasaccharide phosphate protein transferase xanthan article bacterial virulence biosynthesis carboxy terminal sequence gene gene function mutant nonhuman nucleotide sequence priority journal xanthomonas campestris Base Sequence Genes, Bacterial Molecular Sequence Data Mutation Plant Diseases Polysaccharides, Bacterial Uridine Diphosphate Glucose Virulence Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490- 2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non- gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan biosynthesis reduce the aggressiveness of X. campestris against the plant. Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferreiro, D.U. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen http://hdl.handle.net/20.500.12110/paper_00219193_v180_n7_p1607_Katzen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	gene product glucose 1 phosphate glucuronic acid lipid mannose pentasaccharide phosphate protein transferase xanthan article bacterial virulence biosynthesis carboxy terminal sequence gene gene function mutant nonhuman nucleotide sequence priority journal xanthomonas campestris Base Sequence Genes, Bacterial Molecular Sequence Data Mutation Plant Diseases Polysaccharides, Bacterial Uridine Diphosphate Glucose Virulence Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris
spellingShingle	gene product glucose 1 phosphate glucuronic acid lipid mannose pentasaccharide phosphate protein transferase xanthan article bacterial virulence biosynthesis carboxy terminal sequence gene gene function mutant nonhuman nucleotide sequence priority journal xanthomonas campestris Base Sequence Genes, Bacterial Molecular Sequence Data Mutation Plant Diseases Polysaccharides, Bacterial Uridine Diphosphate Glucose Virulence Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris Katzen, Federico Ferreiro, Diego U. Ielmini, María Verónica Ielpi, Luis Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
topic_facet	gene product glucose 1 phosphate glucuronic acid lipid mannose pentasaccharide phosphate protein transferase xanthan article bacterial virulence biosynthesis carboxy terminal sequence gene gene function mutant nonhuman nucleotide sequence priority journal xanthomonas campestris Base Sequence Genes, Bacterial Molecular Sequence Data Mutation Plant Diseases Polysaccharides, Bacterial Uridine Diphosphate Glucose Virulence Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris
description	Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490- 2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non- gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan biosynthesis reduce the aggressiveness of X. campestris against the plant.
author	Katzen, Federico Ferreiro, Diego U. Ielmini, María Verónica Ielpi, Luis
author_facet	Katzen, Federico Ferreiro, Diego U. Ielmini, María Verónica Ielpi, Luis
author_sort	Katzen, Federico
title	Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
title_short	Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
title_full	Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
title_fullStr	Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
title_full_unstemmed	Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
title_sort	xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen http://hdl.handle.net/20.500.12110/paper_00219193_v180_n7_p1607_Katzen
work_keys_str_mv	AT katzenfederico xanthomonascampestrispvcampestrisgummutantseffectsonxanthanbiosynthesisandplantvirulence AT ferreirodiegou xanthomonascampestrispvcampestrisgummutantseffectsonxanthanbiosynthesisandplantvirulence AT ielminimariaveronica xanthomonascampestrispvcampestrisgummutantseffectsonxanthanbiosynthesisandplantvirulence AT ielpiluis xanthomonascampestrispvcampestrisgummutantseffectsonxanthanbiosynthesisandplantvirulence
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Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence

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