Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum

A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clon...

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Autores principales: Petroni, Eberto Alejandro, Ielpi, Luis
Publicado: 1996
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni
http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni
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spelling paper:paper_00219193_v178_n16_p4814_Petroni2023-06-08T14:43:10Z Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum Petroni, Eberto Alejandro Ielpi, Luis acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common. Fil:Petroni, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic acetylsalicylic acid
bacterial dna
bacterial enzyme
polymer
polysaccharide
transferase
xanthan
acetobacter
article
bacterial gene
carbohydrate synthesis
controlled study
gene isolation
nonhuman
nucleotide sequence
open reading frame
priority journal
xanthomonas
Algorithms
Amino Acid Sequence
Carbohydrate Sequence
Cloning, Molecular
Conjugation, Genetic
Conserved Sequence
Escherichia coli
Gene Library
Genes, Bacterial
Genetic Complementation Test
Gluconacetobacter xylinus
Mannosyltransferases
Molecular Sequence Data
Mutagenesis
Open Reading Frames
Polysaccharides, Bacterial
Recombinant Proteins
Sequence Homology, Amino Acid
Xanthomonas
Acetobacter
Bacteria (microorganisms)
Gluconacetobacter xylinus
Xanthomonas
Xanthomonas campestris
spellingShingle acetylsalicylic acid
bacterial dna
bacterial enzyme
polymer
polysaccharide
transferase
xanthan
acetobacter
article
bacterial gene
carbohydrate synthesis
controlled study
gene isolation
nonhuman
nucleotide sequence
open reading frame
priority journal
xanthomonas
Algorithms
Amino Acid Sequence
Carbohydrate Sequence
Cloning, Molecular
Conjugation, Genetic
Conserved Sequence
Escherichia coli
Gene Library
Genes, Bacterial
Genetic Complementation Test
Gluconacetobacter xylinus
Mannosyltransferases
Molecular Sequence Data
Mutagenesis
Open Reading Frames
Polysaccharides, Bacterial
Recombinant Proteins
Sequence Homology, Amino Acid
Xanthomonas
Acetobacter
Bacteria (microorganisms)
Gluconacetobacter xylinus
Xanthomonas
Xanthomonas campestris
Petroni, Eberto Alejandro
Ielpi, Luis
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
topic_facet acetylsalicylic acid
bacterial dna
bacterial enzyme
polymer
polysaccharide
transferase
xanthan
acetobacter
article
bacterial gene
carbohydrate synthesis
controlled study
gene isolation
nonhuman
nucleotide sequence
open reading frame
priority journal
xanthomonas
Algorithms
Amino Acid Sequence
Carbohydrate Sequence
Cloning, Molecular
Conjugation, Genetic
Conserved Sequence
Escherichia coli
Gene Library
Genes, Bacterial
Genetic Complementation Test
Gluconacetobacter xylinus
Mannosyltransferases
Molecular Sequence Data
Mutagenesis
Open Reading Frames
Polysaccharides, Bacterial
Recombinant Proteins
Sequence Homology, Amino Acid
Xanthomonas
Acetobacter
Bacteria (microorganisms)
Gluconacetobacter xylinus
Xanthomonas
Xanthomonas campestris
description A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common.
author Petroni, Eberto Alejandro
Ielpi, Luis
author_facet Petroni, Eberto Alejandro
Ielpi, Luis
author_sort Petroni, Eberto Alejandro
title Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
title_short Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
title_full Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
title_fullStr Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
title_full_unstemmed Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
title_sort isolation and nucleotide sequence of the gdp-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from acetobacter xylinum
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni
http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni
work_keys_str_mv AT petroniebertoalejandro isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum
AT ielpiluis isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum
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