Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clon...
Guardado en:
Autores principales: | , |
---|---|
Publicado: |
1996
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
Aporte de: |
id |
paper:paper_00219193_v178_n16_p4814_Petroni |
---|---|
record_format |
dspace |
spelling |
paper:paper_00219193_v178_n16_p4814_Petroni2023-06-08T14:43:10Z Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum Petroni, Eberto Alejandro Ielpi, Luis acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common. Fil:Petroni, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris |
spellingShingle |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris Petroni, Eberto Alejandro Ielpi, Luis Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
topic_facet |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris |
description |
A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common. |
author |
Petroni, Eberto Alejandro Ielpi, Luis |
author_facet |
Petroni, Eberto Alejandro Ielpi, Luis |
author_sort |
Petroni, Eberto Alejandro |
title |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_short |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_full |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_fullStr |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_full_unstemmed |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_sort |
isolation and nucleotide sequence of the gdp-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from acetobacter xylinum |
publishDate |
1996 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
work_keys_str_mv |
AT petroniebertoalejandro isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum AT ielpiluis isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum |
_version_ |
1768542301694459904 |