Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.

Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasile...

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Guardado en:
Detalles Bibliográficos
Publicado: 1990
Materias:
restriction endonuclease
article
Azospirillum
gene expression
genetic engineering
membrane
nonhuman
priority journal
Rhizobium radiobacter
sequence homology
virulence
Agrobacterium tumefaciens
Azospirillum brasilense
Rhizobium
Sinorhizobium meliloti
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v172_n5_p2563_Altabe
http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00219193_v172_n5_p2563_Altabe
record_format dspace
spelling paper:paper_00219193_v172_n5_p2563_Altabe2023-06-08T14:43:09Z Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp. restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum Azospirillum brasilense Rhizobium Sinorhizobium meliloti Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed β-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v172_n5_p2563_Altabe http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic restriction endonuclease
article
Azospirillum
gene expression
genetic engineering
membrane
nonhuman
priority journal
Rhizobium radiobacter
sequence homology
virulence
Agrobacterium tumefaciens
Azospirillum
Azospirillum brasilense
Rhizobium
Sinorhizobium meliloti
spellingShingle restriction endonuclease
article
Azospirillum
gene expression
genetic engineering
membrane
nonhuman
priority journal
Rhizobium radiobacter
sequence homology
virulence
Agrobacterium tumefaciens
Azospirillum
Azospirillum brasilense
Rhizobium
Sinorhizobium meliloti
Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
topic_facet restriction endonuclease
article
Azospirillum
gene expression
genetic engineering
membrane
nonhuman
priority journal
Rhizobium radiobacter
sequence homology
virulence
Agrobacterium tumefaciens
Azospirillum
Azospirillum brasilense
Rhizobium
Sinorhizobium meliloti
description Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed β-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure.
title Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_short Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_full Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_fullStr Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_full_unstemmed Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_sort expression of the agrobacterium tumefaciens chvb virulence region in azospirillum spp.
publishDate 1990
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v172_n5_p2563_Altabe
http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
_version_ 1768541830170804224

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