Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 16...
Guardado en:
Autor principal: | |
---|---|
Publicado: |
1986
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v168_n1_p270_Ugalde http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde |
Aporte de: |
id |
paper:paper_00219193_v168_n1_p270_Ugalde |
---|---|
record_format |
dspace |
spelling |
paper:paper_00219193_v168_n1_p270_Ugalde2023-06-08T14:43:07Z Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti Ugalde, Rodolfo Augusto galactose galacturonic acid polysaccharide uridine diphosphate galactose uridine diphosphate glucuronic acid article nonhuman priority journal Sinorhizobium meliloti Medicago sativa Rhizobium Scophthalmus rhombus Sinorhizobium meliloti Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v168_n1_p270_Ugalde http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
galactose galacturonic acid polysaccharide uridine diphosphate galactose uridine diphosphate glucuronic acid article nonhuman priority journal Sinorhizobium meliloti Medicago sativa Rhizobium Scophthalmus rhombus Sinorhizobium meliloti |
spellingShingle |
galactose galacturonic acid polysaccharide uridine diphosphate galactose uridine diphosphate glucuronic acid article nonhuman priority journal Sinorhizobium meliloti Medicago sativa Rhizobium Scophthalmus rhombus Sinorhizobium meliloti Ugalde, Rodolfo Augusto Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
topic_facet |
galactose galacturonic acid polysaccharide uridine diphosphate galactose uridine diphosphate glucuronic acid article nonhuman priority journal Sinorhizobium meliloti Medicago sativa Rhizobium Scophthalmus rhombus Sinorhizobium meliloti |
description |
Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end. |
author |
Ugalde, Rodolfo Augusto |
author_facet |
Ugalde, Rodolfo Augusto |
author_sort |
Ugalde, Rodolfo Augusto |
title |
Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
title_short |
Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
title_full |
Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
title_fullStr |
Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
title_full_unstemmed |
Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
title_sort |
biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in rhizobium meliloti |
publishDate |
1986 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v168_n1_p270_Ugalde http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde |
work_keys_str_mv |
AT ugalderodolfoaugusto biosynthesisofagalactoseandgalacturonicacidcontainingpolysaccharideinrhizobiummeliloti |
_version_ |
1768544351348064256 |