Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphory...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218561_v60_n1_p418_Cenzano http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano |
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paper:paper_00218561_v60_n1_p418_Cenzano2023-06-08T14:42:17Z Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. © 2011 American Chemical Society. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218561_v60_n1_p418_Cenzano http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum |
| spellingShingle |
electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| topic_facet |
electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum |
| description |
There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. © 2011 American Chemical Society. |
| title |
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| title_short |
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| title_full |
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| title_fullStr |
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| title_full_unstemmed |
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| title_sort |
lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218561_v60_n1_p418_Cenzano http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano |
| _version_ |
1768541549377880064 |