Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies

Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands...

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Detalles Bibliográficos
Autor principal: Bossi, Mariano Luis
Publicado: 2013
Materias:
fluorescence
optical nanoscopy
photochemistry
photochromism
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00212148_v53_n5_p267_Belov
http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00212148_v53_n5_p267_Belov
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spelling paper:paper_00212148_v53_n5_p267_Belov2023-06-08T14:42:02Z Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies Bossi, Mariano Luis fluorescence optical nanoscopy photochemistry photochromism Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Fil:Bossi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00212148_v53_n5_p267_Belov http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic fluorescence
optical nanoscopy
photochemistry
photochromism
spellingShingle fluorescence
optical nanoscopy
photochemistry
photochromism
Bossi, Mariano Luis
Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
topic_facet fluorescence
optical nanoscopy
photochemistry
photochromism
description Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
author Bossi, Mariano Luis
author_facet Bossi, Mariano Luis
author_sort Bossi, Mariano Luis
title Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_short Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_full Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_fullStr Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_full_unstemmed Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_sort photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
publishDate 2013
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00212148_v53_n5_p267_Belov
http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
work_keys_str_mv AT bossimarianoluis photoswitchingemissionwithrhodaminespiroamidesforsuperresolutionfluorescencenanoscopies
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