Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America

Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mito...

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Detalles Bibliográficos
Autor principal:	Confalonieri, Viviana Andrea
Publicado:	2012
Materias:	Argentina Nematoda New species Puma concolor Ribosomal DNA Taxonomy Trichinella patagoniensis cytochrome c oxidase cytochrome c oxidase 1 genomic DNA ribosome DNA RNA 5S spacer DNA unclassified drug carnivore DNA mammal nematode Neotropical Region new species taxonomy animal experiment animal model animal tissue article bioassay cat controlled study cross breeding cytochrome c oxidase 1 gene DNA sequence domestic pig experimental infection female gene genetic variability genotype in vitro study male mouse nonhuman nucleotide sequence phylogeny polymerase chain reaction puma rat reproductive isolation sequence alignment sequence analysis South America Trichinella trichinosis Animals Female Genetic Speciation Larva Male Mice Molecular Sequence Data Phylogeny Puma Rats Rats, Wistar Swine Swine Diseases Trichinellosis Animalia Mammalia Mus Rattus Sus scrofa domestica Trichinella britovi Trichinella murrelli Trichinella spiralis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v42_n10_p903_Krivokapich http://hdl.handle.net/20.500.12110/paper_00207519_v42_n10_p903_Krivokapich
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00207519_v42_n10_p903_Krivokapich
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spelling	paper:paper_00207519_v42_n10_p903_Krivokapich2023-06-08T14:41:28Z Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America Confalonieri, Viviana Andrea Argentina Nematoda New species Puma concolor Ribosomal DNA Taxonomy Trichinella patagoniensis cytochrome c oxidase cytochrome c oxidase 1 genomic DNA ribosome DNA RNA 5S spacer DNA unclassified drug carnivore DNA mammal nematode Neotropical Region new species taxonomy animal experiment animal model animal tissue article bioassay carnivore cat controlled study cross breeding cytochrome c oxidase 1 gene DNA sequence domestic pig experimental infection female gene genetic variability genotype in vitro study male mouse nonhuman nucleotide sequence phylogeny polymerase chain reaction puma rat reproductive isolation sequence alignment sequence analysis South America Trichinella Trichinella patagoniensis trichinosis Animals Argentina Female Genetic Speciation Larva Male Mice Molecular Sequence Data Phylogeny Puma Rats Rats, Wistar Swine Swine Diseases Trichinella Trichinellosis Argentina Animalia Mammalia Mus Nematoda Puma concolor Rattus Sus scrofa domestica Trichinella Trichinella britovi Trichinella murrelli Trichinella spiralis Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp. © 2012 Australian Society for Parasitology Inc. Fil:Confalonieri, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v42_n10_p903_Krivokapich http://hdl.handle.net/20.500.12110/paper_00207519_v42_n10_p903_Krivokapich
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Argentina Nematoda New species Puma concolor Ribosomal DNA Taxonomy Trichinella patagoniensis cytochrome c oxidase cytochrome c oxidase 1 genomic DNA ribosome DNA RNA 5S spacer DNA unclassified drug carnivore DNA mammal nematode Neotropical Region new species taxonomy animal experiment animal model animal tissue article bioassay carnivore cat controlled study cross breeding cytochrome c oxidase 1 gene DNA sequence domestic pig experimental infection female gene genetic variability genotype in vitro study male mouse nonhuman nucleotide sequence phylogeny polymerase chain reaction puma rat reproductive isolation sequence alignment sequence analysis South America Trichinella Trichinella patagoniensis trichinosis Animals Argentina Female Genetic Speciation Larva Male Mice Molecular Sequence Data Phylogeny Puma Rats Rats, Wistar Swine Swine Diseases Trichinella Trichinellosis Argentina Animalia Mammalia Mus Nematoda Puma concolor Rattus Sus scrofa domestica Trichinella Trichinella britovi Trichinella murrelli Trichinella spiralis
spellingShingle	Argentina Nematoda New species Puma concolor Ribosomal DNA Taxonomy Trichinella patagoniensis cytochrome c oxidase cytochrome c oxidase 1 genomic DNA ribosome DNA RNA 5S spacer DNA unclassified drug carnivore DNA mammal nematode Neotropical Region new species taxonomy animal experiment animal model animal tissue article bioassay carnivore cat controlled study cross breeding cytochrome c oxidase 1 gene DNA sequence domestic pig experimental infection female gene genetic variability genotype in vitro study male mouse nonhuman nucleotide sequence phylogeny polymerase chain reaction puma rat reproductive isolation sequence alignment sequence analysis South America Trichinella Trichinella patagoniensis trichinosis Animals Argentina Female Genetic Speciation Larva Male Mice Molecular Sequence Data Phylogeny Puma Rats Rats, Wistar Swine Swine Diseases Trichinella Trichinellosis Argentina Animalia Mammalia Mus Nematoda Puma concolor Rattus Sus scrofa domestica Trichinella Trichinella britovi Trichinella murrelli Trichinella spiralis Confalonieri, Viviana Andrea Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
topic_facet	Argentina Nematoda New species Puma concolor Ribosomal DNA Taxonomy Trichinella patagoniensis cytochrome c oxidase cytochrome c oxidase 1 genomic DNA ribosome DNA RNA 5S spacer DNA unclassified drug carnivore DNA mammal nematode Neotropical Region new species taxonomy animal experiment animal model animal tissue article bioassay carnivore cat controlled study cross breeding cytochrome c oxidase 1 gene DNA sequence domestic pig experimental infection female gene genetic variability genotype in vitro study male mouse nonhuman nucleotide sequence phylogeny polymerase chain reaction puma rat reproductive isolation sequence alignment sequence analysis South America Trichinella Trichinella patagoniensis trichinosis Animals Argentina Female Genetic Speciation Larva Male Mice Molecular Sequence Data Phylogeny Puma Rats Rats, Wistar Swine Swine Diseases Trichinella Trichinellosis Argentina Animalia Mammalia Mus Nematoda Puma concolor Rattus Sus scrofa domestica Trichinella Trichinella britovi Trichinella murrelli Trichinella spiralis
description	Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp. © 2012 Australian Society for Parasitology Inc.
author	Confalonieri, Viviana Andrea
author_facet	Confalonieri, Viviana Andrea
author_sort	Confalonieri, Viviana Andrea
title	Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_short	Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_full	Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_fullStr	Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_full_unstemmed	Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_sort	trichinella patagoniensis n. sp. (nematoda), a new encapsulated species infecting carnivorous mammals in south america
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v42_n10_p903_Krivokapich http://hdl.handle.net/20.500.12110/paper_00207519_v42_n10_p903_Krivokapich
work_keys_str_mv	AT confalonierivivianaandrea trichinellapatagoniensisnspnematodaanewencapsulatedspeciesinfectingcarnivorousmammalsinsouthamerica
_version_	1768541591602987008

Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America

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