Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease

Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might...

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Autores principales:	Burgos, Juan Miguel, Duffy, Tomás, Piccinali, Romina Valeria, Schijman, Alejandro Gabriel
Publicado:	2007
Materias:	Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line human infant major clinical study newborn prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors South America
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v37_n12_p1319_Burgos http://hdl.handle.net/20.500.12110/paper_00207519_v37_n12_p1319_Burgos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00207519_v37_n12_p1319_Burgos2023-06-08T14:41:26Z Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease Burgos, Juan Miguel Duffy, Tomás Piccinali, Romina Valeria Schijman, Alejandro Gabriel Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc. Fil:Burgos, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Duffy, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piccinali, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v37_n12_p1319_Burgos http://hdl.handle.net/20.500.12110/paper_00207519_v37_n12_p1319_Burgos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi
spellingShingle	Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi Burgos, Juan Miguel Duffy, Tomás Piccinali, Romina Valeria Schijman, Alejandro Gabriel Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
topic_facet	Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi
description	Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc.
author	Burgos, Juan Miguel Duffy, Tomás Piccinali, Romina Valeria Schijman, Alejandro Gabriel
author_facet	Burgos, Juan Miguel Duffy, Tomás Piccinali, Romina Valeria Schijman, Alejandro Gabriel
author_sort	Burgos, Juan Miguel
title	Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
title_short	Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
title_full	Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
title_fullStr	Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
title_full_unstemmed	Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
title_sort	direct molecular profiling of minicircle signatures and lineages of trypanosoma cruzi bloodstream populations causing congenital chagas disease
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v37_n12_p1319_Burgos http://hdl.handle.net/20.500.12110/paper_00207519_v37_n12_p1319_Burgos
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_version_	1768544487237222400

Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease

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