Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi
Trypanosoma cruzi ribosomes from epimastigote forms were purified as determined by electron microscopy and isoelectrofocusing was used to analyse this purified ribosome fraction. Silver stained gels revealed that acidic proteins are present in at least 10 different isoforms, in accord with previous...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v31_n10_p1032_Gomez http://hdl.handle.net/20.500.12110/paper_00207519_v31_n10_p1032_Gomez |
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paper:paper_00207519_v31_n10_p1032_Gomez2023-06-08T14:41:25Z Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi Gómez, Eliana Beatriz Medina, Gabriel Ruben Isoelectrofocusing P proteins Protein phosphorylation Ribosome Trypanosoma acid protein casein kinase II isoprotein ribosome protein article controlled study enzyme activity isoelectric focusing nonhuman protein phosphorylation regulatory mechanism ribosome Trypanosoma cruzi Animals Blotting, Western Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Microscopy, Electron Phosphorylation Protozoan Proteins Recombinant Proteins Ribosomal Proteins Ribosomes Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosoma cruzi ribosomes from epimastigote forms were purified as determined by electron microscopy and isoelectrofocusing was used to analyse this purified ribosome fraction. Silver stained gels revealed that acidic proteins are present in at least 10 different isoforms, in accord with previous cloning studies. To detect phosphorylation, in vitro phosphorylation assays using the recombinant protein TcP2β-mbp were carried out. The results showed that T. cruzi cytosolic fraction possesses protein kinase activity able to phosphorylate the recombinant protein. Purified ribosomes contain protein kinases that could also phosphorylate the recombinant protein TcP2β-mbp. Labelling parasites with [32Pi] in a phosphate free medium demonstrated that ribosome proteins, recognised with a specific mouse antiserum against recombinant TcP2β proteins, are phosphorylated in vivo. All these results suggest that in vivo phosphorylation of ribosome TcP2β proteins are mediated by protein kinase(s) not yet identified. © 2001 Australian Society for Parasitology Inc. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medina, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v31_n10_p1032_Gomez http://hdl.handle.net/20.500.12110/paper_00207519_v31_n10_p1032_Gomez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Isoelectrofocusing P proteins Protein phosphorylation Ribosome Trypanosoma acid protein casein kinase II isoprotein ribosome protein article controlled study enzyme activity isoelectric focusing nonhuman protein phosphorylation regulatory mechanism ribosome Trypanosoma cruzi Animals Blotting, Western Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Microscopy, Electron Phosphorylation Protozoan Proteins Recombinant Proteins Ribosomal Proteins Ribosomes Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Isoelectrofocusing P proteins Protein phosphorylation Ribosome Trypanosoma acid protein casein kinase II isoprotein ribosome protein article controlled study enzyme activity isoelectric focusing nonhuman protein phosphorylation regulatory mechanism ribosome Trypanosoma cruzi Animals Blotting, Western Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Microscopy, Electron Phosphorylation Protozoan Proteins Recombinant Proteins Ribosomal Proteins Ribosomes Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Gómez, Eliana Beatriz Medina, Gabriel Ruben Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| topic_facet |
Isoelectrofocusing P proteins Protein phosphorylation Ribosome Trypanosoma acid protein casein kinase II isoprotein ribosome protein article controlled study enzyme activity isoelectric focusing nonhuman protein phosphorylation regulatory mechanism ribosome Trypanosoma cruzi Animals Blotting, Western Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Microscopy, Electron Phosphorylation Protozoan Proteins Recombinant Proteins Ribosomal Proteins Ribosomes Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| description |
Trypanosoma cruzi ribosomes from epimastigote forms were purified as determined by electron microscopy and isoelectrofocusing was used to analyse this purified ribosome fraction. Silver stained gels revealed that acidic proteins are present in at least 10 different isoforms, in accord with previous cloning studies. To detect phosphorylation, in vitro phosphorylation assays using the recombinant protein TcP2β-mbp were carried out. The results showed that T. cruzi cytosolic fraction possesses protein kinase activity able to phosphorylate the recombinant protein. Purified ribosomes contain protein kinases that could also phosphorylate the recombinant protein TcP2β-mbp. Labelling parasites with [32Pi] in a phosphate free medium demonstrated that ribosome proteins, recognised with a specific mouse antiserum against recombinant TcP2β proteins, are phosphorylated in vivo. All these results suggest that in vivo phosphorylation of ribosome TcP2β proteins are mediated by protein kinase(s) not yet identified. © 2001 Australian Society for Parasitology Inc. |
| author |
Gómez, Eliana Beatriz Medina, Gabriel Ruben |
| author_facet |
Gómez, Eliana Beatriz Medina, Gabriel Ruben |
| author_sort |
Gómez, Eliana Beatriz |
| title |
Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| title_short |
Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| title_full |
Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| title_fullStr |
Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| title_full_unstemmed |
Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi |
| title_sort |
acidic ribosomal p proteins are phosphorylated in trypanosoma cruzi |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v31_n10_p1032_Gomez http://hdl.handle.net/20.500.12110/paper_00207519_v31_n10_p1032_Gomez |
| work_keys_str_mv |
AT gomezelianabeatriz acidicribosomalpproteinsarephosphorylatedintrypanosomacruzi AT medinagabrielruben acidicribosomalpproteinsarephosphorylatedintrypanosomacruzi |
| _version_ |
1768542205974151168 |