Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase

1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain...

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Autor principal:	Batlle, Alcira María del Carmen
Publicado:	1987
Materias:	4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v19_n4_p373_Koopmann http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0020711X_v19_n4_p373_Koopmann
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spelling	paper:paper_0020711X_v19_n4_p373_Koopmann2023-06-08T14:41:09Z Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase Batlle, Alcira María del Carmen 4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc 1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+, and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CvSH. 3. 3. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. 4. 4. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity. © 1987. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v19_n4_p373_Koopmann http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc
spellingShingle	4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc Batlle, Alcira María del Carmen Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
topic_facet	4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc
description	1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+, and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CvSH. 3. 3. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. 4. 4. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity. © 1987.
author	Batlle, Alcira María del Carmen
author_facet	Batlle, Alcira María del Carmen
author_sort	Batlle, Alcira María del Carmen
title	Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
title_short	Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
title_full	Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
title_fullStr	Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
title_full_unstemmed	Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
title_sort	biosynthesis of porphyrins in rhodopseudomonas palustris-vi. the effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
publishDate	1987
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v19_n4_p373_Koopmann http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann
work_keys_str_mv	AT batllealciramariadelcarmen biosynthesisofporphyrinsinrhodopseudomonaspalustrisvitheeffectofmetalsthiolsandotherreagentsontheactivityofuroporphyrinogendecarboxylase
_version_	1768545677890027520

Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase

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