Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase
1. 1. Resealed erythrocytes ghosts are potential in vivo carriers of exogenous therapeutic enzymes. The present work is part of studies directed toward the use of enzymes encapsulated within artificial or natural cellular carriers, in enzyme replacement therapy in porphyrias. 2. 2. Lead intoxication...
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1983
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p447_Bustos http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p447_Bustos |
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paper:paper_0020711X_v15_n3_p447_Bustos2023-06-08T14:41:06Z Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase porphobilinogen synthase animal cell blood and hemopoietic system drug blood level drug delivery system drug efficacy enzyme replacement enzyme therapy erythrocyte ghost human human cell intoxication porphyria therapy 1. 1. Resealed erythrocytes ghosts are potential in vivo carriers of exogenous therapeutic enzymes. The present work is part of studies directed toward the use of enzymes encapsulated within artificial or natural cellular carriers, in enzyme replacement therapy in porphyrias. 2. 2. Lead intoxication was chosen as a model system for these experiments. 3. 3. The optimum conditions for both the preparation of erythrocyte ghosts and encapsulation of δ-aminolaevulinate dehydratase (ALA-D) were studied in detail and a very good yield procedure for entrapment of exogenous highly purified human blood ALA-D, into both normal and defective erythrocytes was developed. 4. 4. The capacity of ALA-D defective cells of lead intoxicated patients and animals was brought within the normal range, in vitro, either by introducing ALA-D into them or by mixing with ALA-D loaded erythrocyte ghosts. 5. 5. Red blood cell ghosts entrapment is therefore a promising approach to the delivery of ALA-D when applying enzyme replacement therapy in lead poisoning. © 1983. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p447_Bustos http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p447_Bustos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
porphobilinogen synthase animal cell blood and hemopoietic system drug blood level drug delivery system drug efficacy enzyme replacement enzyme therapy erythrocyte ghost human human cell intoxication porphyria therapy |
| spellingShingle |
porphobilinogen synthase animal cell blood and hemopoietic system drug blood level drug delivery system drug efficacy enzyme replacement enzyme therapy erythrocyte ghost human human cell intoxication porphyria therapy Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| topic_facet |
porphobilinogen synthase animal cell blood and hemopoietic system drug blood level drug delivery system drug efficacy enzyme replacement enzyme therapy erythrocyte ghost human human cell intoxication porphyria therapy |
| description |
1. 1. Resealed erythrocytes ghosts are potential in vivo carriers of exogenous therapeutic enzymes. The present work is part of studies directed toward the use of enzymes encapsulated within artificial or natural cellular carriers, in enzyme replacement therapy in porphyrias. 2. 2. Lead intoxication was chosen as a model system for these experiments. 3. 3. The optimum conditions for both the preparation of erythrocyte ghosts and encapsulation of δ-aminolaevulinate dehydratase (ALA-D) were studied in detail and a very good yield procedure for entrapment of exogenous highly purified human blood ALA-D, into both normal and defective erythrocytes was developed. 4. 4. The capacity of ALA-D defective cells of lead intoxicated patients and animals was brought within the normal range, in vitro, either by introducing ALA-D into them or by mixing with ALA-D loaded erythrocyte ghosts. 5. 5. Red blood cell ghosts entrapment is therefore a promising approach to the delivery of ALA-D when applying enzyme replacement therapy in lead poisoning. © 1983. |
| title |
Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| title_short |
Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| title_full |
Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| title_fullStr |
Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| title_full_unstemmed |
Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| title_sort |
enzyme replacement therapy in porphyrias-iii: potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase |
| publishDate |
1983 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p447_Bustos http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p447_Bustos |
| _version_ |
1768542442003365888 |