Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes

1. 1. Different types of neutral and negative, large, small and multilamellar liposomes were prepared and conditions for entrapping partially and highly purified preparations of blood and bovine ALA-dehydratase were studied. 2. 2. Gel filtration on Sepharose 4B, resolved liposomes formed in the abse...

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Autores principales:	Espinola, Luis Guillermo, Batlle, Alcira María del Carmen
Publicado:	1983
Materias:	liposome porphobilinogen synthase biological model blood and hemopoietic system drug delivery system drug efficacy drug therapy enzyme replacement enzyme therapy human porphyria therapy
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p439_Espinola http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p439_Espinola
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0020711X_v15_n3_p439_Espinola
record_format	dspace
spelling	paper:paper_0020711X_v15_n3_p439_Espinola2023-06-08T14:41:06Z Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes Espinola, Luis Guillermo Batlle, Alcira María del Carmen liposome porphobilinogen synthase biological model blood and hemopoietic system drug delivery system drug efficacy drug therapy enzyme replacement enzyme therapy human porphyria therapy 1. 1. Different types of neutral and negative, large, small and multilamellar liposomes were prepared and conditions for entrapping partially and highly purified preparations of blood and bovine ALA-dehydratase were studied. 2. 2. Gel filtration on Sepharose 4B, resolved liposomes formed in the absence of enzyme from free enzyme, when both were chromatographed 1-2 hr after mixing, showing that ALA-D did not become associated with liposomes. 3. 3. Preparation of liposomes in the presence of ALA-D resulted in partial entrapment of the enzyme and separation of the protein-containing liposomes from excess free protein was achieved by Sepharose 4B gel filtration. 4. 4. Part of the activity of ALA-D associated with liposomes was latent, and it could only be detected after treatment with Triton X-100, which, at the concentration used, disrupted the spherules and did not affect ALA-D activity. Some activity was also measured in intact ALA-D loaded liposomes. 5. 5. Of all preparations of liposomes, best entrapment values for ALA-D were consistently obtained with negatively charged large multilamellar vesicles, (-LMV (ALA-D)). Considerable less yield was associated with negative small unilamellar vesicles (-SUV) and neutral large unilamellar vesicles (nLUV). © 1983. Fil:Espinola, L.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p439_Espinola http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p439_Espinola
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	liposome porphobilinogen synthase biological model blood and hemopoietic system drug delivery system drug efficacy drug therapy enzyme replacement enzyme therapy human porphyria therapy
spellingShingle	liposome porphobilinogen synthase biological model blood and hemopoietic system drug delivery system drug efficacy drug therapy enzyme replacement enzyme therapy human porphyria therapy Espinola, Luis Guillermo Batlle, Alcira María del Carmen Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
topic_facet	liposome porphobilinogen synthase biological model blood and hemopoietic system drug delivery system drug efficacy drug therapy enzyme replacement enzyme therapy human porphyria therapy
description	1. 1. Different types of neutral and negative, large, small and multilamellar liposomes were prepared and conditions for entrapping partially and highly purified preparations of blood and bovine ALA-dehydratase were studied. 2. 2. Gel filtration on Sepharose 4B, resolved liposomes formed in the absence of enzyme from free enzyme, when both were chromatographed 1-2 hr after mixing, showing that ALA-D did not become associated with liposomes. 3. 3. Preparation of liposomes in the presence of ALA-D resulted in partial entrapment of the enzyme and separation of the protein-containing liposomes from excess free protein was achieved by Sepharose 4B gel filtration. 4. 4. Part of the activity of ALA-D associated with liposomes was latent, and it could only be detected after treatment with Triton X-100, which, at the concentration used, disrupted the spherules and did not affect ALA-D activity. Some activity was also measured in intact ALA-D loaded liposomes. 5. 5. Of all preparations of liposomes, best entrapment values for ALA-D were consistently obtained with negatively charged large multilamellar vesicles, (-LMV (ALA-D)). Considerable less yield was associated with negative small unilamellar vesicles (-SUV) and neutral large unilamellar vesicles (nLUV). © 1983.
author	Espinola, Luis Guillermo Batlle, Alcira María del Carmen
author_facet	Espinola, Luis Guillermo Batlle, Alcira María del Carmen
author_sort	Espinola, Luis Guillermo
title	Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
title_short	Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
title_full	Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
title_fullStr	Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
title_full_unstemmed	Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes
title_sort	enzyme replacement therapy in porphyrias-ii: entrapment of δ-aminolaevulinate dehydratase in liposomes
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v15_n3_p439_Espinola http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p439_Espinola
work_keys_str_mv	AT espinolaluisguillermo enzymereplacementtherapyinporphyriasiientrapmentofdaminolaevulinatedehydrataseinliposomes AT batllealciramariadelcarmen enzymereplacementtherapyinporphyriasiientrapmentofdaminolaevulinatedehydrataseinliposomes
_version_	1768542870225027072

Enzyme replacement therapy in porphyrias-II: Entrapment of δ-aminolaevulinate dehydratase in liposomes

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