Nitric oxide and macrophage antiviral extrinsic activity

In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated,...

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Publicado:	1999
Materias:	n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00192805_v98_n3_p363_Benencia http://hdl.handle.net/20.500.12110/paper_00192805_v98_n3_p363_Benencia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00192805_v98_n3_p363_Benencia
record_format	dspace
spelling	paper:paper_00192805_v98_n3_p363_Benencia2023-06-08T14:40:17Z Nitric oxide and macrophage antiviral extrinsic activity n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-L-arginine acetate (L-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of L-NMA was reversed by the addition of L-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, L-NMA treatment resulted in the aggravation of HSV1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00192805_v98_n3_p363_Benencia http://hdl.handle.net/20.500.12110/paper_00192805_v98_n3_p363_Benencia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication
spellingShingle	n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication Nitric oxide and macrophage antiviral extrinsic activity
topic_facet	n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication
description	In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-L-arginine acetate (L-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of L-NMA was reversed by the addition of L-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, L-NMA treatment resulted in the aggravation of HSV1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection.
title	Nitric oxide and macrophage antiviral extrinsic activity
title_short	Nitric oxide and macrophage antiviral extrinsic activity
title_full	Nitric oxide and macrophage antiviral extrinsic activity
title_fullStr	Nitric oxide and macrophage antiviral extrinsic activity
title_full_unstemmed	Nitric oxide and macrophage antiviral extrinsic activity
title_sort	nitric oxide and macrophage antiviral extrinsic activity
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00192805_v98_n3_p363_Benencia http://hdl.handle.net/20.500.12110/paper_00192805_v98_n3_p363_Benencia
_version_	1768544486680428544

Nitric oxide and macrophage antiviral extrinsic activity

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