From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish
The enzyme aromatase, responsible for the conversion of C19 androgens to C18 estrogens, exists as two paralogue copies in teleost fish: Cyp19a1a mostly expressed in the gonads, referred as gonadal aromatase, and Cyp19a1b, mostly expressed in the brain, accordingly known as brain aromatase. The neura...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018506X_v89_n_p176_Ramallo http://hdl.handle.net/20.500.12110/paper_0018506X_v89_n_p176_Ramallo |
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paper:paper_0018506X_v89_n_p176_Ramallo2023-06-08T14:39:35Z From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish Ramallo, Martín R. Somoza, Gustavo Manuel Pandolfi, Matías aromatase messenger RNA androgen aromatase messenger RNA adult aggression amino acid sequence animal cell animal experiment animal tissue Article brain ventricle cichlid controlled study Cyp19a1b gene estrogen synthesis female forebrain gene gene expression hypophysis macroglia male Neotropics nonhuman protein analysis protein expression real time polymerase chain reaction social behavior social environment social status teleost agonistic behavior animal brain cichlid dominant-subordinate relationship genetics physiology territoriality Agonistic Behavior Androgens Animals Aromatase Brain Cichlids Dominance-Subordination Gene Expression Male RNA, Messenger Social Environment Territoriality The enzyme aromatase, responsible for the conversion of C19 androgens to C18 estrogens, exists as two paralogue copies in teleost fish: Cyp19a1a mostly expressed in the gonads, referred as gonadal aromatase, and Cyp19a1b, mostly expressed in the brain, accordingly known as brain aromatase. The neural localization of Cyp19a1b is greatly contained within the social behavior network and mesolimbic reward system in fish, suggesting a strong role of estrogen synthesis in the regulation of social behavior. In this work we aimed to analyze the variation in cyp19a1b expression in brain and pituitary of males of a highly social cichlid, Cichlasoma dimerus (locally known as chanchita), and its relation with inter-individual variability in agonistic behavior in a communal social environment. We first characterized chanchita's cyp19a1b mRNA and deduced amino acid sequence, which showed a high degree of conservation when compared to other teleost brain aromatase sequences, and its tissue expression patterns. Within the brain, Cyp19a1b was solely detected at putative radial glial cells of the forebrain, close to the brain ventricles. We then studied the relative expression levels of cyp19a1b by Real Time PCR in the brain and pituitary of males of different social status, territorial vs. non-territorial, and its relationship with an index of agonistic behavior. We found that even though, brain aromatase expression did not differ between types of males, pituitary cyp19a1b expression levels positively correlated with the index of agonistic behavior. This suggests a novel role of the pituitary in the regulation of social behavior by local estrogen synthesis. © 2017 Elsevier Inc. Fil:Ramallo, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Somoza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018506X_v89_n_p176_Ramallo http://hdl.handle.net/20.500.12110/paper_0018506X_v89_n_p176_Ramallo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aromatase messenger RNA androgen aromatase messenger RNA adult aggression amino acid sequence animal cell animal experiment animal tissue Article brain ventricle cichlid controlled study Cyp19a1b gene estrogen synthesis female forebrain gene gene expression hypophysis macroglia male Neotropics nonhuman protein analysis protein expression real time polymerase chain reaction social behavior social environment social status teleost agonistic behavior animal brain cichlid dominant-subordinate relationship genetics physiology territoriality Agonistic Behavior Androgens Animals Aromatase Brain Cichlids Dominance-Subordination Gene Expression Male RNA, Messenger Social Environment Territoriality |
| spellingShingle |
aromatase messenger RNA androgen aromatase messenger RNA adult aggression amino acid sequence animal cell animal experiment animal tissue Article brain ventricle cichlid controlled study Cyp19a1b gene estrogen synthesis female forebrain gene gene expression hypophysis macroglia male Neotropics nonhuman protein analysis protein expression real time polymerase chain reaction social behavior social environment social status teleost agonistic behavior animal brain cichlid dominant-subordinate relationship genetics physiology territoriality Agonistic Behavior Androgens Animals Aromatase Brain Cichlids Dominance-Subordination Gene Expression Male RNA, Messenger Social Environment Territoriality Ramallo, Martín R. Somoza, Gustavo Manuel Pandolfi, Matías From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| topic_facet |
aromatase messenger RNA androgen aromatase messenger RNA adult aggression amino acid sequence animal cell animal experiment animal tissue Article brain ventricle cichlid controlled study Cyp19a1b gene estrogen synthesis female forebrain gene gene expression hypophysis macroglia male Neotropics nonhuman protein analysis protein expression real time polymerase chain reaction social behavior social environment social status teleost agonistic behavior animal brain cichlid dominant-subordinate relationship genetics physiology territoriality Agonistic Behavior Androgens Animals Aromatase Brain Cichlids Dominance-Subordination Gene Expression Male RNA, Messenger Social Environment Territoriality |
| description |
The enzyme aromatase, responsible for the conversion of C19 androgens to C18 estrogens, exists as two paralogue copies in teleost fish: Cyp19a1a mostly expressed in the gonads, referred as gonadal aromatase, and Cyp19a1b, mostly expressed in the brain, accordingly known as brain aromatase. The neural localization of Cyp19a1b is greatly contained within the social behavior network and mesolimbic reward system in fish, suggesting a strong role of estrogen synthesis in the regulation of social behavior. In this work we aimed to analyze the variation in cyp19a1b expression in brain and pituitary of males of a highly social cichlid, Cichlasoma dimerus (locally known as chanchita), and its relation with inter-individual variability in agonistic behavior in a communal social environment. We first characterized chanchita's cyp19a1b mRNA and deduced amino acid sequence, which showed a high degree of conservation when compared to other teleost brain aromatase sequences, and its tissue expression patterns. Within the brain, Cyp19a1b was solely detected at putative radial glial cells of the forebrain, close to the brain ventricles. We then studied the relative expression levels of cyp19a1b by Real Time PCR in the brain and pituitary of males of different social status, territorial vs. non-territorial, and its relationship with an index of agonistic behavior. We found that even though, brain aromatase expression did not differ between types of males, pituitary cyp19a1b expression levels positively correlated with the index of agonistic behavior. This suggests a novel role of the pituitary in the regulation of social behavior by local estrogen synthesis. © 2017 Elsevier Inc. |
| author |
Ramallo, Martín R. Somoza, Gustavo Manuel Pandolfi, Matías |
| author_facet |
Ramallo, Martín R. Somoza, Gustavo Manuel Pandolfi, Matías |
| author_sort |
Ramallo, Martín R. |
| title |
From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| title_short |
From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| title_full |
From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| title_fullStr |
From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| title_full_unstemmed |
From molecule to behavior: Brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a Neotropical cichlid fish |
| title_sort |
from molecule to behavior: brain aromatase (cyp19a1b) characterization, expression analysis and its relation with social status and male agonistic behavior in a neotropical cichlid fish |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018506X_v89_n_p176_Ramallo http://hdl.handle.net/20.500.12110/paper_0018506X_v89_n_p176_Ramallo |
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