Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution
The photochemistry of 6-(hydroxymethyl)pterin (HPT; 1) in aqueous solution (pH 5-6) was investigated by irradiation at 350 nm at room temperature. The photochemical reactions of the acidic form 1a were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chr...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018019X_v89_n6_p1090_Thomas http://hdl.handle.net/20.500.12110/paper_0018019X_v89_n6_p1090_Thomas |
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paper:paper_0018019X_v89_n6_p1090_Thomas2023-06-08T14:39:10Z Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution Erra Balsells, Rosa Acidity High performance liquid chromatography Hydrogen peroxide Negative ions Photochemical reactions Photooxidation 6-(hydroxymethyl)pterin Photoinduced formation Reactive oxygen species Organic compounds 6 hydroxymethylpterin acid protein hydrogen peroxide pterin derivative reactive oxygen metabolite singlet oxygen superoxide unclassified drug aqueous solution article enzyme chemistry high performance liquid chromatography irradiation photochemistry photolysis priority journal quantum yield room temperature thin layer chromatography ultraviolet spectrophotometry The photochemistry of 6-(hydroxymethyl)pterin (HPT; 1) in aqueous solution (pH 5-6) was investigated by irradiation at 350 nm at room temperature. The photochemical reactions of the acidic form 1a were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and enzymatic methods for the determination of the superoxide anion radical (O2 .-) and hydrogen peroxide (H2O2). When 1a is exposed to UV-A radiation, the intermediates 4 and 4′ are formed reacting with O2 to yield 6-formylpterin (FPT; 5) and 6-carboxypterin (CPT; 6). under formation of O 2 .- and H2O2 (Scheme 3). The quantum yields of the disappearance of HPT (1a) and of the formation of the photoproducts 5 and 6 were determined. HPT was investigated for its efficiency in singlet-oxygen (1O2) production in acidic aqueous solution. The corresponding quantum yield of 1O2 production (ΦΔ) was 0.15±0.02, as measured by the 1O2 luminescence in the near-IR (1270 nm) upon continuous excitation of the sensitizer. However, 1O2 does not participate in the actual photooxidation of HPT (1a) to FPT (5) and CPT (6). © 2006 Verlag Helvetica Chimica Acta AG. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018019X_v89_n6_p1090_Thomas http://hdl.handle.net/20.500.12110/paper_0018019X_v89_n6_p1090_Thomas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acidity High performance liquid chromatography Hydrogen peroxide Negative ions Photochemical reactions Photooxidation 6-(hydroxymethyl)pterin Photoinduced formation Reactive oxygen species Organic compounds 6 hydroxymethylpterin acid protein hydrogen peroxide pterin derivative reactive oxygen metabolite singlet oxygen superoxide unclassified drug aqueous solution article enzyme chemistry high performance liquid chromatography irradiation photochemistry photolysis priority journal quantum yield room temperature thin layer chromatography ultraviolet spectrophotometry |
| spellingShingle |
Acidity High performance liquid chromatography Hydrogen peroxide Negative ions Photochemical reactions Photooxidation 6-(hydroxymethyl)pterin Photoinduced formation Reactive oxygen species Organic compounds 6 hydroxymethylpterin acid protein hydrogen peroxide pterin derivative reactive oxygen metabolite singlet oxygen superoxide unclassified drug aqueous solution article enzyme chemistry high performance liquid chromatography irradiation photochemistry photolysis priority journal quantum yield room temperature thin layer chromatography ultraviolet spectrophotometry Erra Balsells, Rosa Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| topic_facet |
Acidity High performance liquid chromatography Hydrogen peroxide Negative ions Photochemical reactions Photooxidation 6-(hydroxymethyl)pterin Photoinduced formation Reactive oxygen species Organic compounds 6 hydroxymethylpterin acid protein hydrogen peroxide pterin derivative reactive oxygen metabolite singlet oxygen superoxide unclassified drug aqueous solution article enzyme chemistry high performance liquid chromatography irradiation photochemistry photolysis priority journal quantum yield room temperature thin layer chromatography ultraviolet spectrophotometry |
| description |
The photochemistry of 6-(hydroxymethyl)pterin (HPT; 1) in aqueous solution (pH 5-6) was investigated by irradiation at 350 nm at room temperature. The photochemical reactions of the acidic form 1a were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and enzymatic methods for the determination of the superoxide anion radical (O2 .-) and hydrogen peroxide (H2O2). When 1a is exposed to UV-A radiation, the intermediates 4 and 4′ are formed reacting with O2 to yield 6-formylpterin (FPT; 5) and 6-carboxypterin (CPT; 6). under formation of O 2 .- and H2O2 (Scheme 3). The quantum yields of the disappearance of HPT (1a) and of the formation of the photoproducts 5 and 6 were determined. HPT was investigated for its efficiency in singlet-oxygen (1O2) production in acidic aqueous solution. The corresponding quantum yield of 1O2 production (ΦΔ) was 0.15±0.02, as measured by the 1O2 luminescence in the near-IR (1270 nm) upon continuous excitation of the sensitizer. However, 1O2 does not participate in the actual photooxidation of HPT (1a) to FPT (5) and CPT (6). © 2006 Verlag Helvetica Chimica Acta AG. |
| author |
Erra Balsells, Rosa |
| author_facet |
Erra Balsells, Rosa |
| author_sort |
Erra Balsells, Rosa |
| title |
Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| title_short |
Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| title_full |
Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| title_fullStr |
Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| title_full_unstemmed |
Photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| title_sort |
photoinduced formation of reactive oxygen species from the acid form of 6-(hydroxymethyl)pterin in aqueous solution |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0018019X_v89_n6_p1090_Thomas http://hdl.handle.net/20.500.12110/paper_0018019X_v89_n6_p1090_Thomas |
| work_keys_str_mv |
AT errabalsellsrosa photoinducedformationofreactiveoxygenspeciesfromtheacidformof6hydroxymethylpterininaqueoussolution |
| _version_ |
1768545630013095936 |