Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster
Understanding the genetic architecture of complex traits requires identification of the underlying genes and characterization of gene-by-gene and genotype-by-environment interactions. Behaviors that mediate interactions between organisms and their environment are complex traits expected to be especi...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166731_v174_n3_p1349_Sambandan http://hdl.handle.net/20.500.12110/paper_00166731_v174_n3_p1349_Sambandan |
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paper:paper_00166731_v174_n3_p1349_Sambandan2023-06-08T14:38:41Z Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster animal behavior animal cell animal experiment animal tissue article avoidance behavior controlled study developmental genetics Drosophila melanogaster female gene gene expression gene identification gene insertion gene interaction gene mutation genetic epistasis gt1 gene heterozygosity male nonhuman odor priority journal smelling transposon Animals Behavior, Animal DNA Transposable Elements Drosophila melanogaster Epistasis, Genetic Genes, Insect Mutagenesis, Insertional Mutation Odors Smell Drosophila melanogaster Understanding the genetic architecture of complex traits requires identification of the underlying genes and characterization of gene-by-gene and genotype-by-environment interactions. Behaviors that mediate interactions between organisms and their environment are complex traits expected to be especially sensitive to environmental conditions. Previous studies on the olfactory avoidance response of Drosophila melanogaster showed that the genetic architecture of this model behavior depends on epistatic networks of pleiotropic genes. We performed a screen of 1339 co-isogenic p[GT1]-element insertion lines to identify novel genes that contribute to odor-guided behavior and identified 55 candidate genes with known p[GT1]-element insertion sites. Characterization of the expression profiles of 10 p[GT1]-element insertion lines showed that the effects of the transposon insertions are often dependent on developmental stage and that hypomorphic mutations in developmental genes can elicit profound adult behavioral deficits. We assessed epistasis among these genes by constructing all possible double heterozygotes and measuring avoidance responses under two stimulus conditions. We observed enhancer and suppressor effects among subsets of these P-element-tagged genes, and surprisingly, epistatic interactions shifted with changes in the concentration of the olfactory stimulus. Our results show that the manifestation of epistatic networks dynamically changes with alterations in the environment. Copyright © 2006 by the Genetics Society of America. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166731_v174_n3_p1349_Sambandan http://hdl.handle.net/20.500.12110/paper_00166731_v174_n3_p1349_Sambandan |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal behavior animal cell animal experiment animal tissue article avoidance behavior controlled study developmental genetics Drosophila melanogaster female gene gene expression gene identification gene insertion gene interaction gene mutation genetic epistasis gt1 gene heterozygosity male nonhuman odor priority journal smelling transposon Animals Behavior, Animal DNA Transposable Elements Drosophila melanogaster Epistasis, Genetic Genes, Insect Mutagenesis, Insertional Mutation Odors Smell Drosophila melanogaster |
| spellingShingle |
animal behavior animal cell animal experiment animal tissue article avoidance behavior controlled study developmental genetics Drosophila melanogaster female gene gene expression gene identification gene insertion gene interaction gene mutation genetic epistasis gt1 gene heterozygosity male nonhuman odor priority journal smelling transposon Animals Behavior, Animal DNA Transposable Elements Drosophila melanogaster Epistasis, Genetic Genes, Insect Mutagenesis, Insertional Mutation Odors Smell Drosophila melanogaster Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| topic_facet |
animal behavior animal cell animal experiment animal tissue article avoidance behavior controlled study developmental genetics Drosophila melanogaster female gene gene expression gene identification gene insertion gene interaction gene mutation genetic epistasis gt1 gene heterozygosity male nonhuman odor priority journal smelling transposon Animals Behavior, Animal DNA Transposable Elements Drosophila melanogaster Epistasis, Genetic Genes, Insect Mutagenesis, Insertional Mutation Odors Smell Drosophila melanogaster |
| description |
Understanding the genetic architecture of complex traits requires identification of the underlying genes and characterization of gene-by-gene and genotype-by-environment interactions. Behaviors that mediate interactions between organisms and their environment are complex traits expected to be especially sensitive to environmental conditions. Previous studies on the olfactory avoidance response of Drosophila melanogaster showed that the genetic architecture of this model behavior depends on epistatic networks of pleiotropic genes. We performed a screen of 1339 co-isogenic p[GT1]-element insertion lines to identify novel genes that contribute to odor-guided behavior and identified 55 candidate genes with known p[GT1]-element insertion sites. Characterization of the expression profiles of 10 p[GT1]-element insertion lines showed that the effects of the transposon insertions are often dependent on developmental stage and that hypomorphic mutations in developmental genes can elicit profound adult behavioral deficits. We assessed epistasis among these genes by constructing all possible double heterozygotes and measuring avoidance responses under two stimulus conditions. We observed enhancer and suppressor effects among subsets of these P-element-tagged genes, and surprisingly, epistatic interactions shifted with changes in the concentration of the olfactory stimulus. Our results show that the manifestation of epistatic networks dynamically changes with alterations in the environment. Copyright © 2006 by the Genetics Society of America. |
| title |
Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| title_short |
Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| title_full |
Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| title_fullStr |
Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| title_full_unstemmed |
Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster |
| title_sort |
dynamic genetic interactions determine odor-guided behavior in drosophila melanogaster |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166731_v174_n3_p1349_Sambandan http://hdl.handle.net/20.500.12110/paper_00166731_v174_n3_p1349_Sambandan |
| _version_ |
1768544162666250240 |