Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C
Sliced interrenal tissue from Bufo arenarum H. was incubated without exogenous substrate (to determine endogenous corticosterone (B) and progesterone), as well as in the presence of [3H]pregnenolone ([3H]Pre) and [3H]corticosterone, at 28 and at 37°C. When yields of radiometabolites were analyzed as...
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1983
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v51_n1_p138_Ceballos http://hdl.handle.net/20.500.12110/paper_00166480_v51_n1_p138_Ceballos |
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paper:paper_00166480_v51_n1_p138_Ceballos2023-06-08T14:38:23Z Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C Ceballos, Nora Raquel Cozza, Eduardo Néstor 18 hydroxycorticosterone aldosterone corticosterone pregnenolone analysis of variance animal article biosynthesis metabolism toad 18-Hydroxycorticosterone Aldosterone Analysis of Variance Animal Bufo arenarum Corticosterone Pregnenolone Support, Non-U.S. Gov't Sliced interrenal tissue from Bufo arenarum H. was incubated without exogenous substrate (to determine endogenous corticosterone (B) and progesterone), as well as in the presence of [3H]pregnenolone ([3H]Pre) and [3H]corticosterone, at 28 and at 37°C. When yields of radiometabolites were analyzed as such, [3H]Pre at 28°C was found to be a good precursor for 18-hydroxycorticosterone (18-HO-B), aldosterone (aldo), and an unknown N very similar but not equal to 19-hydroxycorticosterone. However, [3H]Pre at 28°C was not a good precursor for corticosterone. When the same tritiated substrate was incubated at 37°C, the yields of 18-HO-B and N diminished significantly, aldo remained as it was at 28°C, and B increased. [3H]B was a poor precursor to aldo, 18-HO-B, and N, irrespective of incubation temperatures, but a good precursor to material with the mobility of 11-dehydro-B and an aldo-like compound X. Measurement of endogenous B and progesterone pools followed by calculations of specific activities lead to the conclusion that these differences should not be ascribed to artifactual competitions between precursor pools for enzyme systems. This species is thus characteristic in its capacity to bypass, at least at 28°C and under the in vitro conditions employed, corticosterone during aldo biosynthesis. Furthermore, the vast ambient temperature ranges to which this and many other amphibian species are exposed and the temperature dependence observed in corticoidogenesis of B. arenarum suggest that corticoidogenic studies in these species should be carried out at more than one temperature. © 1983. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v51_n1_p138_Ceballos http://hdl.handle.net/20.500.12110/paper_00166480_v51_n1_p138_Ceballos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
18 hydroxycorticosterone aldosterone corticosterone pregnenolone analysis of variance animal article biosynthesis metabolism toad 18-Hydroxycorticosterone Aldosterone Analysis of Variance Animal Bufo arenarum Corticosterone Pregnenolone Support, Non-U.S. Gov't |
| spellingShingle |
18 hydroxycorticosterone aldosterone corticosterone pregnenolone analysis of variance animal article biosynthesis metabolism toad 18-Hydroxycorticosterone Aldosterone Analysis of Variance Animal Bufo arenarum Corticosterone Pregnenolone Support, Non-U.S. Gov't Ceballos, Nora Raquel Cozza, Eduardo Néstor Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| topic_facet |
18 hydroxycorticosterone aldosterone corticosterone pregnenolone analysis of variance animal article biosynthesis metabolism toad 18-Hydroxycorticosterone Aldosterone Analysis of Variance Animal Bufo arenarum Corticosterone Pregnenolone Support, Non-U.S. Gov't |
| description |
Sliced interrenal tissue from Bufo arenarum H. was incubated without exogenous substrate (to determine endogenous corticosterone (B) and progesterone), as well as in the presence of [3H]pregnenolone ([3H]Pre) and [3H]corticosterone, at 28 and at 37°C. When yields of radiometabolites were analyzed as such, [3H]Pre at 28°C was found to be a good precursor for 18-hydroxycorticosterone (18-HO-B), aldosterone (aldo), and an unknown N very similar but not equal to 19-hydroxycorticosterone. However, [3H]Pre at 28°C was not a good precursor for corticosterone. When the same tritiated substrate was incubated at 37°C, the yields of 18-HO-B and N diminished significantly, aldo remained as it was at 28°C, and B increased. [3H]B was a poor precursor to aldo, 18-HO-B, and N, irrespective of incubation temperatures, but a good precursor to material with the mobility of 11-dehydro-B and an aldo-like compound X. Measurement of endogenous B and progesterone pools followed by calculations of specific activities lead to the conclusion that these differences should not be ascribed to artifactual competitions between precursor pools for enzyme systems. This species is thus characteristic in its capacity to bypass, at least at 28°C and under the in vitro conditions employed, corticosterone during aldo biosynthesis. Furthermore, the vast ambient temperature ranges to which this and many other amphibian species are exposed and the temperature dependence observed in corticoidogenesis of B. arenarum suggest that corticoidogenic studies in these species should be carried out at more than one temperature. © 1983. |
| author |
Ceballos, Nora Raquel Cozza, Eduardo Néstor |
| author_facet |
Ceballos, Nora Raquel Cozza, Eduardo Néstor |
| author_sort |
Ceballos, Nora Raquel |
| title |
Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| title_short |
Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| title_full |
Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| title_fullStr |
Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| title_full_unstemmed |
Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C |
| title_sort |
corticoidogenesis in bufo arenarum h. i. in vitro biosynthesis of [3h]pregnenolone and [3h]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°c and 37°c |
| publishDate |
1983 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v51_n1_p138_Ceballos http://hdl.handle.net/20.500.12110/paper_00166480_v51_n1_p138_Ceballos |
| work_keys_str_mv |
AT ceballosnoraraquel corticoidogenesisinbufoarenarumhiinvitrobiosynthesisof3hpregnenoloneand3hcorticosteronemetabolitesandofendogenous3oxo4eneintermediatesat28cand37c AT cozzaeduardonestor corticoidogenesisinbufoarenarumhiinvitrobiosynthesisof3hpregnenoloneand3hcorticosteronemetabolitesandofendogenous3oxo4eneintermediatesat28cand37c |
| _version_ |
1768543163556823040 |